Summary
The complete nucleotide sequences of two chromosomally linked actin genes from the sea urchinStrongylocentrotus franciscanus are presented. The genes are separated by 5.7 kilobases, occur in the same transcriptional orientation, and contain introns in identical positions. The structures and nucleotide sequences of the two genes are extremely similar, suggesting that they arose through a recent duplication. Comparison of the nucleotide sequences of the genes allows inferences to be made about mutational mechanisms active since the duplication event. Whereas point mutations predominate in the coding regions, the introns and flanking DNA are more heavily influenced by a variety of events that cause simultaneous changes in short regions of DNA.
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Albertini AM, Hofer N, Calos MP, Miller JH (1982) On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions. Cell 29:319–328
Aquadro CF, Greenberg BD (1983) Human mitochondrial DNA variation and evolution: analysis of nucleotide sequences from individuals. Genetics 103:287–312
Cooper AD, Crain W (1982) Complete nucleotide sequence of a sea urchin actin gene. Nucleic Acids Res 10:4081–4092
Delaney AD (1982) A DNA sequence handling program. Nucleic Acids Res 10:61–67
Efstratiadis A, Posakony JW, Maniatis T, Lawn RM, O'Connell CO, Spritz RA, Deriel JK, Forget BG, Weissman SM, Slighton JL, Blechl AE, Smithies O, Barelle FE, Shoulder CC, Proudfoot NJ (1980) The structure and evolution of the human β-globin gene family Cell 21:653–668
Fryberg EA, Bond BJ, Hershey ND, Mixter KS, Davidson N (1981) The actin genes ofDrosophila: Protein coding regions are highly conserved but intron positions are not. Cell 24: 107–116
Glickman BW, Ripley LS (1984) Structural intermediates of deletion mutagenesis: a role for palindromic DNA. Proc Natl Acad Sci USA 81:512–516
Grantham R, Gautier C, Govy M, Mercier R, Pave A (1980) Codon catalog usage and the genome hypothesis. Nucleic Acids Res 8:r49-r62
Grula JW, Hall TJ, Hunt JA, Giugni TD, Graham GJ, Davidson EH, Britten RJ (1982) Sea urchin DNA sequence variation and reduced interspecies differences of the less variable DNA sequences. Evolution 36:665–676
Johnson PJ, Foran DR, Moore GP (1983) Organization and evolution of the actin gene family in sea urchins. Mol Cell Biol 3:1924–1933
Kleinsmith LJ, Peters NK, Zeigler ME (1984) Non-muscle actin gene expression during early development. In: Stein G (ed) Recombinant DNA approaches to studying control of cell proliferation. Academic Press, New York, pp 273–301
Maxam A, Gilbert W (1977) A new method for sequencing DNA. Proc Natl Acad Sci USA 74:560–564
Merlino GT, Water RD, Chamberlain JP, Jackson DA, El-Gewely MR, Kleinsmith LJ (1980) Cloning of sea urchin actin gene sequences for use in studying the regulation of actin gene transcription. Proc Natl Acad Sci USA 77:765–769
Moore GP, Moore AR, Grossman LI (1984) The frequency of matching sequences in DNA. J Theor Biol 180:111–122
Nei M (1983) Genetic polymorphism and the role of mutation in evolution. In: Nei M, Koehn R (eds) Evolution of genes and proteins. Sinauer Associates, Sunderland, Massachusettts, pp 165–190
Nellen WC, Donath C, Moos M, Gallwitz D (1981) The nucleotide sequences of the actin genes fromSaccharomyces carlsbergensis andSaccharomyces cerevisiae are identical except for their introns. J Mol Appl Gen 1:239–244
Perler F, Efstratiadis A, Lomedico P, Gilbert W, Kolodner RP, Dodgson J (1980) The evolution of genes: the chicken prepronsulin gene. Cell 20:555–566
Raff RA, Kaufman TC (1983) Embryos, genes and evolution. MacMillan, New York, pp 62–93
Ripley LS (1982) Model for the participation of quasi-palindromic DNA sequences in frameshift mutation. Proc Natl Acad Sci USA 79:4128–4132
Sanchez F, Tobin SL, Rdest V, Zulauf E, McCarthy BJ (1983) TwoDrosophila actin genes in detail: gene structure, protein structure and transcription during development. J Mol Biol 163:533–551
Schuler MA, McOsker P, Keller EB (1983) DNA sequences of two linked actin genes of sea urchin. Mol Cell Biol 3:448–456
Sibley CG, Ahlquist JE (1984) The phylogeny of the hominoid primates, as indicated by DNA-DNA hybridization. J Mol Evol 20:2–15
Soriano P, Szabo P, Bernari G (1982) The scattered distribution of actin genes in the mouse and human genomes. EMBO J 1:579–583
Streisinger G, Okada Y, Emrich J, Newton J, Tsugita A, Terzaghi E, Inouye M (1966) Frameshift mutations and the genetic code. Cold Spring Harbor Symp Quant Biol 31:77–84
Zeigler ME, Kish VM, Kleinsmith LJ (1983) 5 End mapping of two cloned sea urchin actin genes. Fed Proc 42:1970
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Foran, D.R., Johnson, P.J. & Moore, G.P. Evolution of two actin genes in the sea urchinStrongylocentrotus franciscanus . J Mol Evol 22, 108–116 (1985). https://doi.org/10.1007/BF02101689
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DOI: https://doi.org/10.1007/BF02101689