Summary
We have cloned and determined the complete nucleotide sequence of a RAS gene from the yeastSchizosaccharomyces pombe (SP-RAS). The putative RAS protein of 214 amino acids is encoded by two noncontiguous reading frames separated by an intron of 86 bp. The SP-RAS gene product shares extensive homology with the proteins of theSaccharomyces cerevisiae (SC),Dictyostelium, Drosophila, and human RAS genes in its N-terminal region but not in its C-terminal region. The extended C-terminal regions found in the SC-RAS genes have no counterpart in the SP-RAS gene. Thus the RAS genes of these two yeasts are structurally quite distinct. The SP-RAS sequence was expressed in vivo.
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Abbreviations
- bp:
-
base pair
- kb:
-
kilobase pair
- SP-RAS:
-
Schizosaccharomyces pombe RAS
- SC-RAS:
-
Saccharomyces cerevisiae RAS
- v-K-RAS:
-
Kirsten murine sarcoma virus RAS
- v-H-RAS:
-
Harvey murine sarcoma virus RAS
- H-RAS:
-
human RAS
- HMP:
-
high melting point
- LMP:
-
low melting point
- ORF:
-
open reading frame
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Nadin-Davis, S.A., Yang, R.C.A., Narang, S.A. et al. The cloning and characterization of a RAS gene fromSchizosaccharomyces pombe . J Mol Evol 23, 41–51 (1986). https://doi.org/10.1007/BF02100997
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DOI: https://doi.org/10.1007/BF02100997