Summary
Comparative, quantitative Southern analysis of genomic DNA, using single-copy sequence probes, potentially is valuable for phylogenetic analysis. We have examined 27Drosophila species, belonging to two subgenera, seven species groups, and ten subgroups, using a variety of cloned and characterized probes: twelve cloned sequences fromD. melanogaster, two fromD. pseudoobscura, and two fromD. grimshawi. The data are generally congruent with accepted phylogenetic relationships inDrosophila, and confirm or clarify some previously uncertain relationships. The potential and limitations of the method are discussed.
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Abraham G, Scaletta C, Vaughan J (1972) Modified diphenylamine reaction for increased sensitivity. Analytical Biochemistry 49:547–549
Angerer RC, Davidson EH, Britten RJ (1976) Single copy DNA and structural gene sequence relationships among four sea urchin species. Chromosoma 56:213–226
Barnes SR, Webb DA, Dover G (1978) The distribution of satellite and main-band DNA components in themelanogaster species subgroups ofDrosophila. Chromosoma 67:341–363
Barnett T, Pachl C, Gergen J, Wensink P (1980) The isolation and characterization of Drosophila yolk protein genes. Cell 21:729–738
Beverley SM, Wilson AC (1984) Molecular evolution inDrosophila and the higher diptera. II. A time scale for fly evolution. J Mol Evol 21:1–13
Blackman RK, Meselson M (1986) Interspecific nucleotide sequence comparisons used to identify regulatory and structural features of theDrosophila hsp82 gene. J Mol Biol 188:499–515
Bodmer M, Ashburner M (1984) Conservation and change in the DNA sequences coding for alcohol dehydrogenase in sibling species ofDrosophila. Nature 309:425–430
Bond BJ, Davidson N (1986) TheDrosophila melanogaster actin 5C gene uses two transcription initiation sites and three polyadenlyation sites to express multiple mRNA species. Mol Cel Biol 6:2080–2088
Britten RJ (1986) Rates of DNA sequence evolution differ between taxonomic groups. Science 231:1392–1398
Carson HL, Stalker HD (1969) Polytene chromosome relationships in Hawaiian species ofDrosophila. IV. TheD. primaeva subgroup. Univ Texas Publ 6918:85–94
Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995
Coen E, Strachan T, Dover G (1982) Dynamics of concerted evolution of ribosomal DNA and histone gene families in themelanogaster species subgroup ofDrosophila. J Mol Biol 158:17–35
Collier CE, MacIntyre RJ (1977) Micro-complement fixation studies on the evolution of alpha-glycerophosphate dehydrogenase within the genusDrosophila. Proc Natl. Acad Sci USA 74:684–688
Coté B, Bender W, Curtis D, Chovnick A (1986) Molecular mapping of therosy locus inDrosophila melanogaster. Genetics 112:769–783
Cseko YMT, Dower NA, Minoo P, Lowenstein L, Smith GR, Stone J, Sederoff R (1979) Evolution of polypyrimidines inDrosophila. Genetics 92:459–484
Eisses K, van Dijk H, van Delten W (1979) Genetic differentiation within themelanogaster species group of the genusDrosophila (Sophophora). Evolution 33:1063–1068
Fauron CM-R, Wolstenholme D (1980) Extensive diversity amongDrosophila species with respect to nucleotide sequences within the adenine+thymine-rich region of mitochondrial DNA molecules. Nucl Acids Res 8:2439–2452
Fyrberg E, Kindle K, Davidson N (1980) The actin genes of Drosophila: a dispersed multigene family. Cell 19:365–378
Goldberg DA (1980) Isolation and partial characterization of theDrosophila alcohol dehydrogenase gene. Proc Natl Acad Sci 77:5794–5798
Gonzalez A, Cabrera V, Larruga J, Gullon A (1982) Genetic distance in the sibling speciesDrosophila melanogaster, Drosophila simulans andDrosophila mauritiana. Evolution 36:517–522
Griffin-Shea R, Thireos GC, Kafatos FC (1982) Organization of a cluster of four chorion genes inDrosophila and its relationship to developmental expression and amplification. Devel Biol 91:325–336
Hung M-C, Wensink PC (1981) The sequence of theDrosophila melanogaster gene for yolk protein-1. Nucl Acids Res 9:6407–6419
Krimbas CB, Loukas M (1984) Evolution of the obscura groupDrosophila species. I. Salivary chromosomes and quantitative characters inD. subobscura and two closely related species. Heredity 53:469–482
Lachaise D, Tsacas L (1974) LesDrosophilidae des savanes préforestières de la région tropicale de Lamto (Côte d'Ivoire). II. Le peuplement des fruits dePandanus candelabrum (Pandanacées). Ann Univ Abidjan E 7:153–192
Lakovaara S, Saura A, Lankinen P, Pohjola L, Lokki J (1976) The use of isoenzymes in tracing evolution and in classifying Drosophilidae. Zoologica Scripta 5:173–179
Lakovaara S, Saura A (1982) Evolution and speciation in theDrosophila obscura group. In: Ashburner M, Carson HL, Thompson JN (eds) The genetics and biology of Drosophila, vol. 3b. Academic Press, London, NY, pp 1–59
Lemeunier F, Ashburner M (1976) Relationships within themelanogaster species subgroup of the genusDrosophila (Sophophora). II. Phylogenetic relationships between six species based upon polytene chromosome banding sequences. Proc R Soc Lond B 193:275–294
Loukas M, Krimbas CB, Vergini Y (1984) Evolution of the obscura groupDrosophila species. II. Phylogeny of ten species based on electrophoretic data. Heredity 53:483–493
Loukas M, Kafatos FC (1986a) Chromosomal locations of actin genes are conserved between themelanogaster andobscura groups ofDrosophila. Genetica (in press)
Loukas M, Kafatos FC (1986b) The actin loci in the genusDrosophila: Establishment of chromosomal homologies among distantly related species by in situ hybridization. Chromosoma (in press)
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory
Ohnishi S, Kaanishi M, Watanabe TK (1983) Biochemical phylogenies ofDrosophila: protein differences detected by two-dimensional electrophoresis. Genetica 61:55–63
Sibley CG, Ahlquist JE (1984) The phylogeny of the hominoid primates as indicated by DNA-DNA hybridization. J Mol Evol 20:2–15
Snyder M, Hirsh J, Davidson N (1981) The cuticle genes ofDrosophila: A developmentally regulated gene cluster. Cell 25:165–177
Spradling AC (1981) The organization and amplification of two chromosomal domains containingDrosophila chorion genes. Cell 27:193–201
Steinemann M, Pinsker W, Sperlich D (1984) Chromosome homologies within theDrosophila obscura group probed by in situ hybridization. Chromosoma 91:46–53
Strachen T, Coen E, Webb D, Dover G (1982) Modes and rates of change of complex DNA families ofDrosophila. J Mol Biol 158:37–54
Thompson EOP (1980) Amino acid sequences of globin chains and their use in phylogenetic divergence point estimations. In: Sigman DS, Brazier MAB (eds) The evolution of protein structure and function. Academic Press, NY, pp 267–298
Throckmorton LH (1975) The phylogeny, ecology and geography ofDrosophila. In: King RC (ed) Handbook of genetics, vol. 3. Plenum Press, NY, pp 421–469
Wilson AC, Carlson S, White T (1977) Biochemical evolution. Ann Rev Biochem 46:573–639
Zwiebel LJ, Cohn VH, Wright DR, Moore GP (1982) Evolution of single-copy DNA and the ADH gene in seven drosophilids. J Mol Evol 19:62–71
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Loukas, M., Delidakis, C. & Kafatos, F.C. Genomic blot hybridization as a tool of phylogenetic analysis: Evolutionary divergence in the genusDrosophila . J Mol Evol 24, 174–188 (1986). https://doi.org/10.1007/BF02099965
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DOI: https://doi.org/10.1007/BF02099965