Summary
We describe the characteristics of a repetitive DNA sequence from the rainbow trout and related salmonid fishes that is similar to a retroviral long terminal repeat (LTR). The repeat is 160 bp long and contains a region of homology to the LTR of the avian sarcoma virus. Two clones with this repeat from the chum salmon also have a polypurine tract and tRNA binding site, respectively, and these clones may represent the two LTRs of a retrovirus or retroviral-like repetitive element. Copies of the repeat are also adjacent to rainbow trout and chum salmon protamine genes. These repeats may be “solo” LTRs. There appears to be some polymorphism in restriction sites between individual rainbow trout and considerable differences between salmonid fish species when the repeat is used as a probe.
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References
Aiken JM, McKenzie D, Zhao H-Z, States JC, Dixon GH (1983) Sequence homologies in the protamine gene family of the rainbow trout. Nucleic Acids Res 10:7581–7592
Bencini DA, O'Donovan GA, Wild JR (1984) Rapid chemical degradation sequencing. Biotechniques 2:4–5
Boeke JD, Garfinkel DJ, Styles CA, Fink GR (1985) TY elements transpose through an RNA intermediate. Cell 40:491–500
Chen HR, Barker WC (1984) Nucleotide sequences of the retroviral long terminal repeats and their adjacent regions. Nucleic Acids Res 12:1767–1778
Feinberg A, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Garfinkel DJ, Boeke JD, Fink GR (1985) TY element transposition: reverse transcriptase and virus-like particles. Cell 42:507–517
Garger SJ, Griffith OM, Grill LK (1983) Rapid purification of plasmid DNA by a single centrifugation in a two-step cesium chloride-ethidium bromide gradient. Biochem Biophys Res Commun 117:835–842
Jankowski JM, States JC, Dixon GH (1986) Evidence of sequences resembling avian retrovirus long terminal repeats flanking the trout protamine gene. J Mol Evol 23:1–10
Maniatis T, Fritsch E, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor NY
Maxam A, Gilbert W (1980) Sequencing end-labeled DNA with base specific chemical cleavages. Methods Enzymol 65:499–559
McKay DJ, Renaux BS, Dixon GH (1986) Rainbow trout protamines: amino acid sequences of six distinct proteins from a single testis. Eur J Biochem 158:361–366
Moir RD, Dixon GH (1988) Characterization of a protamine gene from the chum salmon (Oncorhynchus keta). J Mol Evol 26:130–148
Rechavi G, Bienz B, Ram D, Ben-Neriah Y, Cohen JB, Zakut R, Givol D (1982) Organization and evolution of immunoglobulin UH gene subgroups. Proc Natl Acad Sci USA 79: 4405–4409
Roeder SG, Fink GR (1980) DNA rearrangements associated with a transposable element in yeast. Cell 21:239
Rotman G, Hin A, Keshet E (1984) “Solo” large terminal repeats (LTR) of an endogenous retrovirus-like gene family (VL30) in the mouse genome. Nucleic Acids Res 12:2273–2282
Sakano H, Maki R, Kurosawa Y, Roeder W, Tonegawa S (1980) Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes. Nature 286:676–683
States JC, Connor W, Wosnick MA, Aiken JM, Gedamu L, Dixon GH (1982) Nucleotide sequence of a protamine component CII gene ofSalmo gairdneri. Nucleic Acids Res 10:4551–4563
Stumph WE, Hodgson CP, Tsai M-J, O'Malley BW (1984) Genomic structure and possible retroviral origin of the chicken CR1 repetitive DNA sequence family. Proc Natl Acad Sci USA 81:6667–6671
Suzuki N, Fujiyoshi T, Maehara Y, Takahasi K, Yamamoto M, Endo H (1986) A new family of LTR-like sequences abundantly expressed in rat tumors. Nucleic Acids Res 14:9271–9289
Temin HM (1982) Function of the retrovirus long terminal repeat. Cell 28:3–5
Varmus HE (1982) Form and function of retroviral proviruses. Science 216:812–820
Weiner AM, Deninger PL, Efstratiadis A (1986) Nonviral retroposons: genes, pseudogenes, and transposable elements generated by the reverse flow of genetic information. Annu Rev Biochem 55:631–661
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Moir, R.D., Dixon, G.H. A repetitive DNA sequence in the salmonid fishes similar to a retroviral long terminal repeat. J Mol Evol 27, 1–7 (1988). https://doi.org/10.1007/BF02099724
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DOI: https://doi.org/10.1007/BF02099724