Abstract
Screening of a large number of bacteria revealed several strains, which utilize 1,4-butanediol and/or 4-hydroxybutyric acid (4HB) as a carbon source for growth and for synthesis of polyhydroxyalkanoic acids (PHA) containing 4HB as one constituent among others (mostly 3-hydroxybutyric acid). However, none of the wild-type strains investigated in this study was able to produce a homopolyester consisting solely of 4HB. Only several poly(3-hydroxybutyric acid)-leaky mutants ofAlcaligenes eutrophus strain JMP222 synthesized poly(4HB) homopolyester, which amounted to approximately 10% (w/w) of the cellular dry matter. If the PHA synthase structural gene ofA. eutrophus strain H16 was expressed in these mutants, the amount of poly(4HB) was increased to approximately 30% (w/w). The occurrence of poly(4HB) was demonstrated by gas chromatographic as well as1H and13C nuclear magnetic resonance spectroscopic analysis.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
A.J. Anderson and E.A. Dawes (1990)Microbiol. Rev. 54 450–472.
A. Steinbüchel (1991)Nachr. Chem. Tech. Lab. 39 1112–1124.
A. Steinbüchel (1991) in D. Byrom (Ed.),Biomaterials, Macmillan, Basingstoke, pp. 123–213.
M. Kunioka, Y. Nakamura, and Y. Doi (1988)Polym. Commun. 29 174–176.
M. Kunioka, Y. Kawaguchi, and Y. Doi (1989)Appl. Microbiol. Biotechnol. 30 569–573.
Y. Doi, M. Kunioka, Y. Nakamura, and Y. Soga (1988)Macromolecules 21 2722–2727.
Y. Doi, A. Segawa, and M. Kunioka (1990)Int. J. Biol. Macromol. 12 106–111.
H. Kimura, Y. Yoshida, and Y. Doi (1992)Biotechnol. Lett. 14 445–450.
M. Hiramitsu, N. Koyama, and Y. Doi (1993)Biotechnol. Lett. 15 461–464.
A. J. Anderson, G. W. Haywood, Dr. R. Williams, and E. A. Dawes (1990) in E. A. Dawes (Ed.),Novel Biodegradable Microbial Polymers, Kluwer Academic, Dordrecht, pp. 119–129.
M. Liebergesell, F. Mayer, and A. Steinbüchel (1993)Appl. Microbiol. Biotechnol. 40 292–300.
Y. Doi, A. Segawa, S. Nakamura, and M. Kunioka (1990) in E. A. Dawes (Ed.),Novel Biodegradable Microbial Polymers, Kluwer Academic, Dordrecht, pp. 37–48.
A. Steinbüchel, El-M. Debzi, R. H. Marchessault, and A. Timm (1993)Appl. Microbiol. Biotechnol. 39 443–449.
S. Nakamura, Y. Doi, and M. Scandola (1992)Macromolecules 25 4237–4241.
R. A. Don and J. M. Pemperton (1981)J. Bacteriol. 145 681–686.
R. Simon, U. Priefer, and A. Pühler (1983)Biotechnology 1 784–791.
P. Schubert, A. Steinbüchel, and H. G. Schlegel (1988)J. Bacteriol. 170 5837–5847.
A. Pries, H. Priefert, N. Krüger, and A. Steinbüchel (1991)J. Bacteriol. 173 5843–5853.
H. G. Schlegel, H. Kaltwasser, and G. Gottschalk (1961)Arch. Mikrobiol. 38 209–222.
H. Brandl, R. A. Gross, R. W. Lenz, and R. C. Fuller (1988)Appl. Environ. Microbiol. 54 1977–1982.
A. Timm, D. Byrom, and A. Steinbüchel (1990)Appl. Microbiol. Biotechnol. 33 296–301.
H. E. Valentin and A. Steinbüchel (1994)Appl. Microbiol. Biotechnol. 40 699–709.
M. M. Bradford (1976)Anal. Biochem. 72 248–254.
U. Schwien and E. Schmidt (1982)Appl. Environ. Microbiol. 44 33–39.
M. Mergeay, C. Houba, and J. Gerrits (1978)Arch. Int. Physiol. Biochim. 86 440–441.
K. Timotius and H. G. Schlegel (1987)Nachr. Akad. Wissensch. Göttingen II Math.-Phys. Klasse 3 15–23.
T. Schmidt and H. G. Schlegel (1989)FEMS Microbiol. Lett. 62 315–328.
A. Timm and A. Steinbüchel (1990)Appl. Environ. Microbiol. 56 3360–3367.
A. Steinbüchel, E. Hustede, M. Liebergesell, A. Timm, U. Pieper, and H. Valentin (1992)FEMS Microbiol. Rev. 103 217–230.
H. E. Valentin, A. Schönebaum, and A. Steinbüchel (1992)Appl. Microbiol. Biotechnol. 36 507–514.
H. E. Valentin, E. Y. Lee, C. Y. Choi, and A. Steinbüchel (1994)Appl. Microbiol. Biotechnol. 40 710–716.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Steinbüchel, A., Valentin, H.E. & Schönebaum, A. Application of recombinant gene technology for production of polyhydroxyalkanoic acids: Biosynthesis of poly(4-hydroxybutyric acid) homopolyester. J Environ Polym Degr 2, 67–74 (1994). https://doi.org/10.1007/BF02074775
Issue Date:
DOI: https://doi.org/10.1007/BF02074775