Abstract
The effect of valinomycin on both, mast cell histamine release and on calcium (45Ca)-uptake processes was examined. Pleural and peritoneal mast cells were purified in isotonic Percoll (pH=7) and mixed populations were used in the experiments.
Valinomycin (10−9–10−5 M) stimulated histamine release in isolated rat mast cells when the incubation medium contained high K+ concentrations (Tris-K+ with 150 mM K+), but not in ohter media such as
Tris-Na+ (120 mM Na+) or Tris-sucrose (300 mM sucrose). In contrast, in the absence of valinomycin, elevated K+ levels in the external environment did not activate mast cell secretion. Optimum response in valinomycin-treated mast cells was obtained when the cells were incubated for 60 min. Also valinomycin (10−5 M) induced substantial inhibition of45Ca-uptake while lower doses (10−9–10−7 M) did not affect or only slightly incrased uptake.
In this paper valinomycin is shown to be a degranulating agent eliciting mediator release in mast cells incubated in the presence of high K+ levels, which does not require extracellular calcium and inhibits45Ca uptake. The possibility that valinomycin acts as a K+ ionophore, as in other secretory systems, is discussed.
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Eleno, N., Botana, L., Segura, C. et al. Valinomycin, a degranulating agent in rat mast cells which inhibits calcium-uptake. Agents and Actions 22, 189–196 (1987). https://doi.org/10.1007/BF02009045
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DOI: https://doi.org/10.1007/BF02009045