Abstract
Irrespective of the indicator system selected, several concentrations of a compound under investigation should be used and a dose-response curve is essential. A standard mutagen as well as an adequate negative control should always be included in the test. At the present state of the art, it is quite, possible that the host-mediated assay will play a significant role in characterizing chemical mutagens and establishing a correlation between carcinogenic and mutagenic substances. Future development in this field will include attempts to localize the site of action of a specific agent by the use of chambers (Fiscor et al. [2]), the development of a single indicator microbial strain that could detect multiple genetic lesions, and use of experimental animals other than the mouse. In addition to the conventional host-mediated assay, the indicator system described in this report will no doubt be used as one of the few available methods for detecting the presence of chemical mutagens and carcinogens in man, by appropriate analysis of body fluids.
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Legator, M.S. Procedure for conducting the host-mediated assay utilizing bacteria (‘Salmonella typhimurium’). Agents and Actions 3, 111–115 (1973). https://doi.org/10.1007/BF01986542
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DOI: https://doi.org/10.1007/BF01986542