Abstract
Nordihydroguaiaretic acid (NDGA) was investigated for its ability to interact with leukotriene B4 receptors on human polymorphonuclear leukocytes (hPMNs).3H-LTB4 binding to specific receptors was reduced in a dose-dependent manner with maximal reduction at 100 μM NDGA and an IC50 of about 50 μM. Binding of another inflammatory stimulus, N-formyl-norleucyl-leucyl-phenylalanine (FNLP) was not affected by similar treatment. Chemotaxis and enzyme release stimulated by LTB4 and oligopeptide were inhibited by NDGA. In addition, LTB4-triggered inflammationin vivo in mice was inhibited by systemic administration of NDGA. These data suggest that LTB4 receptor antagonism may contribute to inhibition of inflammation by NDGA.
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D. W. Goldman and E. J. Goetzl,Specific binding of leukotriene B 4 to receptors on human polymorphonuclear leukocytes. J. Immunol.129, 1600–1604 (1982).
R. A. Kreisle and C. W. Parker,Specific binding of leukotriene B 4 to a receptor on human polymorphonuclear leukocytes. J. Exp. Med.157, 628–641 (1983).
D. W. Goldman and E. J. Goetzl,Heterogeneity of human polymorphonuclear leukocyte receptors for leukotriene B 4-identification of a subset of high affinity receptors that transduce the chemotactic response. J. Exp. Med.159, 1027–1041 (1984).
R. M. Clancy, C. A. Dahinden and T. E. Hugli,Oxidation of leukotrienes at the ω end: demonstration of a receptor for the 20-hydroxy derivative of leukotriene B 4 on human neutrophils and implications for the leukotriene receptors. Proc. Natl. Acad. Sci. USA81, 5729–5733 (1984).
A. H. Lin, P. L. Ruppel and R. R. Gorman,Leukotriene B 4 binding to human neutrophils. Prostaglandins28, 837–849 (1984).
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Maloff, B.L., Fefer, D., Cooke, G.M. et al. Inhibition of LTB4 binding to human neutrophils by nordihydroguaiaretic acid. Agents and Actions 21, 358–360 (1987). https://doi.org/10.1007/BF01966515
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DOI: https://doi.org/10.1007/BF01966515