Abstract
Histamine release from human basophils was investigatedin vitro after removal of cell membrane sialic acid by three different sialidases. Pretreatment of the cells with sialidases fromCl. Perfringens, V. Cholera orInfluenza virus A 2 enhanced histamine release induced by subsequent stimulation of the cells with anti-IgE or the plant lectin Concanavalin A and caused a shift to the left of the dose-response curve for anti-IgE. The enhanced histamine release was reflected in a increased calcium sensitivity, thus suggesting that cell membrane sialic acid might be involved in the calcium fluxes preceeding histamine release. In higher doses the sialidase fromCl. Perfringens caused the cells to release histamine by itself, whereas the sialidases fromV. Cholera andInfluenza virus A 2 in high does inhibited the cell response to Concanavalin A.
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Jensen, C., Dahl, B.T., Norn, S. et al. Enhancement of histamine release from human basophils pretreated with different sialidases. Agents and Actions 18, 499–503 (1986). https://doi.org/10.1007/BF01964953
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DOI: https://doi.org/10.1007/BF01964953