Summary
Electron microscopic observation ofTrypanosoma cruzi epimastigotes reveals the presence of microbody-like structures (microperoxisomes) in which 3,3′-diaminobenzidine (DAB) is peroxidized to electron-opaque material. The role of peroxidase in DAB peroxidation is supported by the enzyme demonstration in disrupted epimastigotes and the microbody-containing cell fractions.
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This investigation was supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and the Special Fund for the Study of Chagas Disease (Subsecretaría de Ciencia y Tecnología de la Nación).
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Docampo, R., de Boiso, J.F., Boveris, A. et al. Localization of peroxidase activity inTrypanosoma cruzi microbodies. Experientia 32, 972–975 (1976). https://doi.org/10.1007/BF01933918
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DOI: https://doi.org/10.1007/BF01933918