Abstract
Insect-parasitic nematodes possess many of the attributes of ideal biological control agents, but intolerance to extreme temperatures can restrict their use. We examined whether heat-shock treatments could improve nematode survival and infectivity at temperatures that normally inhibit their activity (35 and 40°C). Nematodes exposed to a sub-lethal temperature (35°C) for 3 h with a latency period of 1–2 h at 25°C killed insects at 35 and 40°C. Correlative evidence was obtained between increased thermotolerance and the synthesis of 70-kDa heat-shock proteins (hsps). These results provide the first evidence of hsp synthesis in the development of thermotolerance and biological activity in the non-feeding, developmentally arrested, infective juvenile nematodes.
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Selvan, S., Grewal, P.S., Leustek, T. et al. Heat shock enhances thermotolerance of infective juvenile insect-parasitic nematodesHeterorhabditis bacteriophora (Rhabditida: Heterorhabditidae). Experientia 52, 727–730 (1996). https://doi.org/10.1007/BF01925583
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DOI: https://doi.org/10.1007/BF01925583