Abstract
The physical interaction between plasma-membrane lipids and the epidermal growth factor (EGF)-receptor was investigated on single A431 human epidermoid carcinoma cells by monitoring fluorescence resonance energy transfer (FRET) between exogeneously added fluorescein-EGF (donor) and 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine (Bodipy-PC, acceptor) using donor-photobleaching FRET-microscopy. The measured mean FRET-efficiency of 13% is indicative of such a physical interaction and exemplifies the great potential and sensitivity of time-resolved imaging fluorescence microscopy techniques for the study of lipid-receptor interactions on single cells.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
G. Marriott, R. M. Clegg, D. J. Arndt-Jovin, and T. M. Jovin (1991)Biophys. J. 60, 1374–1387.
T. M. Jovin and D. J. Arndt-Jovin (1989)Annu. Rev. Biophys. Biophys. Chem. 18, 271–308.
T. M. Jovin and D. J. Arndt-Jovin (1989) In E. Kohen, J. S. Ploem, and J. G. Hirschberg (Eds.),Cell Structure and Function by Microspectrofluorometry, Academic Press, London, pp. 99–117.
T. M. Jovin, D. J. Arndt-Jovin, G. Marriott, R. M. Clegg, M. Robert-Nicoud, and T. Schormann (1990) in B. Herman and K. Jacobson (Eds.),Optical Microscopy for Biology, Wiley-Liss; New York, pp. 575–602.
T. M. Jovin and D. J. Arndt-Jovin (1992)SPIE Proc. 1439, 109–120.
U. Kubitschek, M. Kircheis, R. Schweitzer-Stenner, W. Dreybrodt, T. M. Jovin, and I. Pecht (1991).Biophys. J. 60, 307–318.
R. M. Clegg, B. Feddersen, E. Gratton, and T. M. Jovin (1992)SPIE Proc. 1604, 448–460.
T. W. J. Gadella, Jr., T. M. Jovin, and R. M. Clegg (1993)Biophys. Chem. 48, 221–239.
P. S. Uster and R. E. Pagano (1986).J. Cell Biol. 103, 1221–1234.
R. Zidovetski, D. A. Johnson, D. J. Arndt-Jovin, and T. M. Jovin (1991)Biochemistry 30, 6162–6166.
C. Höfers, T. M. Jovin, G. Hummer, and D. J. Arndt-Jovin (1993)Bioimaging 1, 107–118.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gadella, T.W.J., Arndt-Jovin, D.J. & Jovin, T.M. Visualization of lipid-receptor interactions on single cells by time-resolved imaging fluorescence microscopy. J Fluoresc 4, 295–298 (1994). https://doi.org/10.1007/BF01881443
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01881443