Summary
Fox and Woese (1975a) have shown that a model of 5S RNA secondary structure similar to the one originally derived forChlorella 5S RNA can be generalized with relatively minor variations to all sequenced 5S RNA molecules, i.e. that corresponding base paired regions can be formed at approximately the same positions. We present experimental data in favour of this hypothesis and show that the points at which ribonucleases T1, T2 and pancreatic ribonuclease cleave six different 5S RNA molecules under ‘mild’ conditions (high ionic strength, low temperature, low RNAase concentration) nearly always fall in the proposed single-stranded regions. We conclude that this model is a good approximation to the conformation of 5S RNA in solution.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bellemare, G., Jordan, B.R., Rocca-Serra, J., Monier, R. (1972). Biochimie54, 1453–1466
Bellemare, G., Vigne, R., Jordan, B.R. (1973). Biochimie55, 29–35
Benhamou, J., Jordan, B.R. (1976). Febs Letters62, 146–149
Benhamou, J., Jourdan, R., Jordan, B.R. (1977). J. Mol. Evol.,9, 279–298
Brownlee, GG. (1972). Determination of Sequences in RNA. North-Holland, Amsterdam
Brownlee, G.G., Sanger, F., Barrell, B.G. (1967). Nature215, 735–736
Delihas, N., Dunn, J.J., Erdmann, V.A. (1975). Febs Letters58, 76–80
Dubuy, B., Weissman, S.M. (1971). J. Biol. Chem.246, 747–761
Forget, B.G., Weissman, S.M. (1967). Science158, 1695–1699
Fox, G.E., Woese, C.R. (1975a) Nature256, 505–507
Fox, G.E., Woese, C.R. (1975b). J. Mol. Evol.6, 61–76
Herr, W., Noller, H.F. (1975). Febs Letters53, 248–252
Hindley, J., Page, S.M. (1972). Febs Letters26, 157–160
Holley, R.W., Apgar, J., Everett, G.A., Madison, J.T., Marquisee, M., Merrill, S.H., Penswick, J.R., Zamir, A. (1965). Science147, 1462–1465
Jordan, B.R. (1971a). J. Theor. Biol.34, 363–378
Jordan, B.R. (1971b). J. Mol. Biol.55, 423–439
Jordan, B.R., Galling, G., Jourdan, R. (1974). J. Mol. Biol.87, 205–225
Kearns, D.R., Wong, Y.P. (1974). J. Mol. Biol.87, 755–774
Litt, M., Ingram, V.M. (1964). Biochemistry3, 560–564
Madison, J.T. (1968). Ann. Rev. Biochem.37, 131–148
Monier, R. (1974). In: Ribosomes. Nomura, Tissieres, Lengyel, (eds.), pp. 141–168. Cold Spring Harbor
Nishikawa, K., Takemura, S. (1974). Febs Letters40, 106–109
Noller, H.F., Herr, W. (1974). J. Mol. Biol.90, 181–184
Richards, E.G. (1969). Eur. J. Biochem.10, 36–42
Tinoco, I., Borer, P.N., Dengler, B., Levine, M.D., Uhlenbeck, O.C., Crothers, D.M., Gralla, J. (1973). Nature New Biology246, 40–41
Vigne, R., Jordan, B.R. (1971). Biochimie53, 981–986
Vigne, R., Jordan, B.R., Monier, R. (1973). J. Mol. Biol.76, 303–311
Wagner, E.K., Ingram, V.M. (1966). Biochemistry5, 3019–3027
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vigne, R., Jordan, B.R. Partial enzyme digestion studies onescherichia coli, pseudomonas, chlorella, drosophila, HeLa and yeast 5S RNAs support a general class of 5S RNA models. J Mol Evol 10, 77–86 (1977). https://doi.org/10.1007/BF01796136
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01796136