Summary
The organization of repetitive and single copy DNA sequences in sea urchin DNA has been examined with the single strand specific nuclease Sl fromAspergillus. Conditions and levels of enzyme were established so that single strand DNA was effectively digested while reassociated divergent repetitive duplexes remained enzyme resistant. About 25% of sea urchin DNA reassociates with repetitive kinetics to form Sl resistant duplexes of two distinct size classes derived from long and short repetitive sequences in the sea urchin genome. Fragments 2,000 nucleotides long were reassociated to Cot 20 and subjected to controlled digestion with Sl nuclease. About half of the resistant duplexes (13% of the DNA) are short, with a mode size of about 300 nucleotide pairs. This class exhibits significant sequence divergence, and principally consists of repetitive sequences which were interspersed with single copy sequences. About one-third of the long duplexes (4% of the DNA) are reduced in size after extensive Sl nuclease digestion to about 300 nucleotide pairs. About two-thirds of the long resistant duplexes (8% of the DNA) remains long after extensive SI nuclease digestion. These long reassociated duplexes are precisely base paired. The short duplexes are imprecisely paired with a melting temperature about 9°C below that of precisely paired duplexes of the same length. The relationship between length of repetitive duplex and precision of repetition is confirmed by an independent method and has been observed in the DNA of a number of species over a wide phylogenetic area.
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Angerer, R.C., Davidson, E.H., Britten, R.J. (1975). Cell 6, 29
Bonner, J., Garrard, W.T., Gottesfeld, J., Holmes, D.S., Sevall, J.S., Wilkes, M. (1974). Cold Spring Harb. Symp. Quant. Biol. 38, 303
Britten, R.J., Davidson, E.H. (1969). Science 165, 349
Britten, R.J., Davidson, E.H. (1971). Quart. Rev. Biol. 46, 111
Britten, R.J., Davidson, E.H. (1976). FASEB, 35, 2151
Britten, R.J., Graham, D.E., Neufeld, B.R. (1974). In: Methods in enzymology, 29E, L. Grossman, K. Moldave, eds., p. 363. New York: Academic Press
Britten, R.J., Kohne, D.E. (1966). Carnegie Inst. Wash. Year Book 65, 78
Britten, R.J., Smith, J.F. (1970). Carnegie Inst. Wash. Year Book 68, 378
Britten, R.J., Smith, J.F. (1971). Carnegie Inst. Wash. Year Book 69, 506
Brown, D.D., Sugimoto, K. (1974). Cold Spring Harb. Symp. Quant. Biol. 38, 501
Brownlee, G.G., Cartwright, E., Brown, D.D. (1974). J. Mol. Biol. 89, 703
Chamberlin, M.E., Britten, R.J., Davidson, E.H. (1975). J.Mol.Biol. 96, 317
Chase, J.W., Richardson, C.C. (1974). J. Biol. Chem. 249, 4553
Crain, W.R., Davidson, E.H., Britten, R.J. (1976). Chromosoma (Berl.), in press
Crothers, D.M., Jallenback, N.R., Zimm, B.H. (1965). J. Mol. Biol. 11, 802
Davidson, E.H., Britten, R.J. (1971). J. Theor. Biol. 32, 123
Davidson, E.H., Britten, R.J. (1973). Quart. Rev. Biol. 48, 565
Davidson, E.H., Graham, D.E., Neufeld, B.R., Chamberlin, M.E., Amenson, C.S., Hough, B.R., Britten, R.J. (1974). Cold Spring Harb. Symp. Quant. Biol. 38, 295
Davidson, E.H., Hough, B.R., Amenson, C.S., Britten, R.J. (1973). J. Mol. Biol. 77, 1
Davidson, E.H., Galau, G.A., Angerer, R.C., Britten, R.J. (1975a). Chromosoma (Berl.) 51, 253
Davidson, E.H., Hough, B.R., Klein, W.H., Britten, R.J. (1975), Cell 4, 217
Efstratiadis, A., Crain, W.R., Britten, R.J., Davidson, E.H., Kafatos, F.C. (1976). Proc. Nat. Acad. Sci. USA, 73, 2289
Galau, G.A., Chamberlin, M.E., Hough, B.R., Britten, R.J., Davidson, E.H. (1976). In: Molecular studies of biological evolution, F.J. Ayala, ed., p. 200. Sunderland, Mass.: Sinauer Press
Goldberg, R.B., Crain, W.R., Ruderman, J.V., Moore, G.P., Barnett, T.R., Higgins, R.C., Gelfand, R.A., Galau, G.A., Britten, R.J., Davidson, E.H. (1975). Chromosoma (Berl.) 51, 225
Goldberg, R.B., Galau, G.A., Britten, R.J., Davidson, E.H. (1973). Proc. Nat. Acad. Sci. USA 70, 3516
Graham, D.E., Neufeld, B.R., Davidson, E.H., Britten, R.J. (1974). Cell 1, 127
Hayes, F.N., Lilly, E.H., Ratliff, R. L., Smith, D.A., and Williams, D.L. (1970). Biopolymers 9, 1105
Hoyer, B.H., van de Velde, N.W., Goodman, M., Roberts, R.B. (1972). Carnegie Inst. Wash. Year Book 71, 260
Klein, W.H., Murphy, W., Attardi, G., Britten, R.J., Davidson, E.H. (1974). Proc. Nat. Acad. Sci. USA, 71, 1785
Kohne, D.E., Chiscon, J.A., and Hoyer, B.H. (1970). Carnegie Inst. Wash. Year Book 69, 488
Manning, J.E., Schmid, C.W., Davidson, N. (1975). Cell 4, 141
Noll, H. (1967). Nature 215, 360
Rice, N. (1972). In evolution of genetic systems, Brookhaven Symp. No. 23, H.H. Smith, ed., p. 44. New York: Gordon and Breach
Rice, N.R. (1974a). Carnegie Inst. Wash. Year Book 73, 1094
Rice, N.R. (1974b). Carnegie Inst. Wash. Year Book 73, 1098
Shenk, T.E., Rhodes, C., Rigby, P.W.J., Berg, P. (1975). Proc. Nat. Acad. Sci. USA 72, 989
Schmid, C.W., Deininger, P.L. (1975). Cell 6, 345
Smith, G. (1974). Cold Spring Harb. Symp. Quant. Biol. 38, 507
Sutton, W.D. (1971). Biochim. Biophys. Acta 240, 522
Vogt, V.M. (1973). Eur. J. Biochem. 33, 192
Wellauer, P.K., Reeder, R.H., Carroll, D., Brown, D.D., Deutch, A., Higashinakagawa, T., Dawid, I.B. (1974. Proc. Nat. Acad. Sci., USA 71, 2823
Wetmur, J.G. (1976). Ann. Rev. Biophys. Bioeng. 5, in press
Wiegand, R.C., Godson, G.N., Radding, C.M. (1975). J. Biol. Chem. 250, 8848
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Britten, R.J., Graham, D.E., Eden, F.C. et al. Evolutionary divergence and length of repetitive sequences in sea urchin DNA. J Mol Evol 9, 1–23 (1976). https://doi.org/10.1007/BF01796119
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DOI: https://doi.org/10.1007/BF01796119