Summary
The complete sequence of the 5S rRNA from the bioluminescent bacterium,Beneckea harveyi has been determined to be p U G C U U G G C G\(\mathop A\limits^{10} \) C C A U A G C G A\(\mathop U\limits^{20} \) U U-G G A C C C A\(\mathop C\limits^{30} \) C U G A (U) C U U C\(\mathop C\limits^{40} \) A U U C C-G A A C\(\mathop U\limits^{50} \) C A G A A G U G A\(\mathop A\limits^{60} \) A C G A A U U A-G\(\mathop C\limits^{70} \) G C C G A U G G U\(\mathop A\limits^{80} \) G U G U G G G G C\(\mathop U\limits^{90} \) U-C C C C A U G U\(\mathop G\limits^{100} \) A G A G U A G G A\(\mathop C\limits^{110} \) A U C G-C C A G G\(\mathop C\limits^{120} \) U (U)OH.
Two sites of sensitivity to ribonuclease T2 cleavage were identified; at A41 and either A54 or A55. Comparison with existing sequence information fromEscherichia coli andPhotobacterium phosphoreum clarifies the amount of diversity among the bioluminescent bacteria and provides further insight into their phylogenetic position. Sequence heterogeneities were encountered and the importance of these in interpreting 5S rRNA data is discussed.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Baumann L, Baumann P (1976) Microbios Letters 3:11–20
Bellemare G, Vigne R, Jordan B (1973) Biochimie 55:29–35
Brownlee GG, Sanger F, Barrell BG (1968) J Mol Biol 34:379–412
Donis-Keller H (1980) Nucleic Acids 8:3133–3142
Donis-Keller H, Maxam AM, Gilbert W (1977) Nucleic Acids 4:2527–2538
Fox GE, Woese CR (1975) Nature 505–507
Hori H, Osawa S (1979) Proc Natl Acad Sci USA 76:381–385
Jordan BR (1971) J Mol Biol 55:423–439
Klotz LC, Komar N, Blanken RL, Mitchell RM (1979) Proc Natl Acad Sci USA 76:4516–4520
Krupp G, Gross HJ (1979) Nucleic Acids 6:3481–3490
Meyhack B, Pace B, Pace NR (1977) Biochemistry 16:5009–5015
Noller HF, Garrett RA (1979) J Mol Biol 132:621–636
Raué HA, Heerschap A, Planta RJ (1976) Eur J Biochem 68:169–176
Reichelt JL, Baumann P (1973) Arch Microbiol 94:283–330
Reichelt JL, Baumann P, Baumann L (1976) Arch Microbiol 110:101–120
Sanger F, Brownlee GG, Barrell BG (1965) J Mol Biol 13:373–398
Uchida T, Bonen L, Schaup H, Lewis BJ, Zablen L, Woese C (1974) J Mol Evol 3:63–77
Woese C, Sogin M, Stahl D, Lewis BJ, Bonen L (1976) J Mol Evol 7:197–213
Woese CR, Pribula CD, Fox GE, Zablen L (1975) J Mol Evol 5:35–46
Wrede P, Erdmann VA (1973) FEBS Letters 33:315–319
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Luehrsen, K.R., Fox, G.E. The nucleotide sequence ofBeneckea harveyi 5S rRNA. J Mol Evol 17, 52–55 (1981). https://doi.org/10.1007/BF01792425
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01792425