Summary
The receptors of peanut agglutinin (PNA),Dolichos biflorus agglutinin (DBA) andUlex europaeus agglutinin I (UEA-I) were localized in intrahepatic cholangiocellular carcinoma, hepatocellular carcinoma, intrahepatic bile ducts and normal, cirrhotic and pericarcinomatous liver using the avidin—biotin—peroxidase complex method. It was found that epithelial cells of normal bile ducts had many UEA-I receptors, fewer DBA receptors and no PNA receptors. The positive rates of PNA, UEA-I and DBA receptors in 18 cases of intrahepatic cholangiocellular carcinoma were 88.9%, 61.1% and 33.3% respectively, which were significantly higher than those in hepatocellular carcinoma (16.0%, 4.0% and 4.0% respectively). Hepatocytes in normal, cirrhotic and pericarcinomatous liver had no receptors for these three lectins. It is suggested that lectin receptor distribution in intrahepatic cholangiocellular carcinoma is obviously different from that in normal bile duct cells and in hepatocellular carcinoma, and might be used as an auxiliary index in its clinical diagnosis.
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ALLISON, R. T. (1986) Lectins in diagnostic histopathology: a review.Med. Lab. Sci. 43, 369–76.
BUAMAH, P. K., CORNELL, C., CASSELL-SMITH, A. J. & SKILLEN, A. W. (1987) Differential reactivity of α-fetoprotein with lectins and its usefulness in the diagnosis of various liver diseases.Clin. Chim. Acta 168, 69–73.
COOPER, H. S. (1984) Lectins as probes in histochemistry and immunohistochemistry. The peanut (Arachis hypogaea) lectin.Hum. Pathol. 15, 904–6.
DAMJANOV, I. (1987) Lectin cytochemistry and histochemistry.Lab. Invest. 57, 5–20.
DICIOCCIO, R. A., BARLOW, J. & MATTA, K. L. (1985) Evaluation of α-L-fucosidase as a marker for primary liver cancer.IRCS Med. Sci. 13, 849–50.
HAKAMORI, S. I. (1985) Aberrant glycosylation in cancer cell membranes as focused on glycolipids: overview and perspectives.Cancer Res. 45, 2405–14.
HSU, S. M., RAINE, L. & FANGER, H. (1981) Use of avidin—biotin—peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.J. Histochem. Cytochem. 29, 577–80.
IRIMURA, T., OTA, D. M. & CLEARY, K. R. (1987)Ulex europaeus agglutinin I-reactive high molecular weight glycoproteins of adenocarcinoma of distal colon and their possible relationship with metastatic potential.Cancer Res. 47, 881–9.
LIEBMAN, H. A., FURIE, B. C., TONG, M. J., BLANCHARD, R. A., LO, K-J., LEE, S-D., COLEMAN, M. S. & FURIE, B. (1984) Des-γ-carboxy(abnormal) prothrombin as a serum marker of primary hepatocellular carcinoma.N. Engl. J. Med. 310, 1427–31.
LIS, H. & SHARON, N. (1986) Lectins as molecules and as tools.Annu. Rev. Biochem. 55, 35–67.
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Zhang, S., Wu, M., Chen, H. et al. Characteristics of the distribution of lectin receptors in intrahepatic cholangiocellular carcinoma. Histochem J 21, 296–300 (1989). https://doi.org/10.1007/BF01757183
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DOI: https://doi.org/10.1007/BF01757183