Summary
Experimental brain tumours were produced in cats by stereotactic implantation of 4 million suspended cells of a rat glioma clone into the internal capsule. Three weeks after implantation a spherical tumour developed with a diameter of up to 10 mm which was surrounded by vasogenic white matter oedema. In untreated animals water content in the peritumoural white matter increased from 69.1 ± 0.9 to 80.0 ± 0.8 ml/100 g w. w., and regional blood flow reciprocally decreased from 32.2. ± 5.6 to 18.9 ± 0.05 ml/100 g/min. A single injection of a crystalline suspension of 10 mg/kg dexamethasone given intramuscularly one week before the animals were killed, led to a significant amelioration of brain oedema. Peritumoural white matter water content decreased to 73.0 ± 0.5 ml/100 g w. w. and blood flow rose to 35.7 ± 2.8 ml/100 g/min. These changes were accompanied by parallel shifts of electrolyte content but they did not correlate with EEG activity, as assessed by Fourier frequency analysis. Corticosteroids did not prevent extravasation of peroxidase or Evans blue across the tumour vessels. The beneficial effect, therefore, is attributed to either an acceleration of resorption or an inhibition of the spread of oedema from the tumour into the peritumoural brain tissue.
Zusammenfassung
Bei Katzen wurden experimentelle Hirntumoren durch stereotaktische Implantation von suspendierten Tumorzellen eines Rattengliomklon in die innere Kapsel hervorgerufen. Innerhalb von drei Wochen entwickelte sich am Implantationsort ein kugelförmiger Tumor mit einem Durchmesser von etwa 10 mm. Um den Tumor herum entstand ein ausgedehntes vasogenes Ödem, das sich in der weißen Substanz ausbreitete. Bei unbehandelten Tieren stieg der Wassergehalt im ödematösen Mark von 69.1 ± 0.9 auf 80.0 ± 0.8 ml/100 g Feuchtgewicht an, und die Durchblutung sank in diesem Gebiet von 32.2 ± 5.6 auf 18.9 ± 0.05 ml/100 g/min ab. Die einmalige intramuskuläre Injektion einer kristallinen Suspension von 10 mg/kg Dexamethason bewirkte innerhalb einer Woche eine signifikante Abnahme des Hirnödems. Der Wassergehalt sank auf 73.0 ± 0.5 ml/100 g Feuchtgewicht und die Durchblutung stieg über den Kontrollwert hinaus auf 35.7 ± 2.8 ml/100 g/min an. Die Veränderungen wurden von entsprechenden Elektrolytverschiebungen begleitet, korrelierten jedoch nicht mit EEG-Veränderungen, die mit der Fourier Frequenz-Analyse quantitativ ausgewertet wurden. Die Kortikosteroid-Behandlung verhinderte nicht die Extravasation von Peroxidase oder Evans Blau aus den Tumorgefäßen. Die therapeutische Wirkung wird deshalb auf eine Beschleunigung der Resorption oder eine Inhibition der Ausbreitung der Ödemflüssigkeit von dem Tumor in das peritumorale Hirngewebe zurückgeführt.
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Matsuoka, Y., Hossmann, K.A. Corticosteroid therapy of experimental tumour oedema. Neurosurg. Rev. 4, 185–190 (1981). https://doi.org/10.1007/BF01743708
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DOI: https://doi.org/10.1007/BF01743708