Summary
Exponential growing Tetrahymena pyriformis organisms were labelled with (3H) uridine or (3H) adenosine. The labelled RNA was extracted and isolated by affinity chromatography on poly-uridylic-acid Sepharose and further analysed by means of sucrose gradient centrifugation and RNase digestion.
Experimental evidence proved the existence of RNase resistant poly adenylic-acid fragments in the RNA of Tetrahymena cells. This poly adenylic-acid segment has a sedimentation rate of 4-5 S and would be localised in the 10-12S region of the RNA which is probably the m-RNA.
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Supported by Stiftung Volkswagenwerk Research Grant No.112273.
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Ron, A., Horovitz, O. & Sarov, I. Fractionation of RNA from tetrahymena by affinity chromatography on poly-u-sepharose. J Mol Evol 8, 137–142 (1976). https://doi.org/10.1007/BF01739099
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DOI: https://doi.org/10.1007/BF01739099