Summary
Multivariate statistical analyses were applied to 16 physical and chemical properties of amino acids. Four of these properties; volume, polarity, isoelectric point (charge), and hydrophobicity were found to explain adequately 96% of the total variance of amino acid attributes. Using these four quantitative measures of amino acid properties, a structural discriminate function in the form of a weighted difference sum of squares equation was developed. The discriminate function is weighted by the location of each particular residue within a given tertiary structure and yields a numerical discriminate or difference value for the replacement of these residues by different amino acids. This resulting discriminate value represents an expression of the perturbation in the local positional environment of a protein when an amino acid substitution occurs. With the use of this structural discriminate function, a residue by residue comparison of the known mammalian myoglobin sequences was carried out in an attempt to elucidate the positions of possible deviations from the known tertiary structure of sperm whale myoglobin. Only 11 of the 153 residue positions in myoglobin demonstrated possible structural deviations. From this analysis, indices of difference were calculated for all amino acid exchanges between the various myoglobins. All comparisons yielded indices of difference that were considerably lower than would be expected if mutations had been fixed at random, even if the organization of the genetic code is taken into consideration. On the basis of these results, it is inferred that some form of selection has acted in the evolution of mammalian myoglobins to favor amino acid substitutions that are compatible with the retention of the original conformation of the protein.
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Air, G.M., Thompson, E.O.P., Richardson, B.J., Sharman, G.B. (1971). Nature229, 391–394
Arnheim, N. (1973). In: The antigens, M. Sela, ed., p. 377, New York: Academic Press
Austin, R.H., Beeson, K.W., Eisenstein, L., Frauenfelder, H., Gunsalus, I.C. (1975). Biochemistry14, 5355–5373
Ayala, F.J. (1976). Molecular evolution, Sunderland, MA: Sinauer
Blackith, R.E., Reyment, R.A. (1971). Multivariate morphometrics, New York: Academic Press
Bogardt, R.A. (1978). Ph.D. Thesis, Indiana University
Bogardt, R.A., Dwulet, F.E., Lehman, L.D., Jones, B.N., Garner, M.H., Garner, W.H. (1976a). Fed. Proc., Fed. Am. Soc. Exp. Biol.35, 1605
Bogardt, R.A., Dwulet, F.E., Lehman, L.D., Jones, B.N., Gurd, F.R.N. (1976b). Biochemistry15, 2497–2602
Botelho, L.H., Gurd, F.R.N. (1978). Biochemistry17, 5188–5196
Botelho, L.H., Friend, S.H., Matthew, J.B., Lehman, L.D., Hanania, G.I.H., Gurd, F.R.N. (1978). Biochemistry17, 5197–5205
Bradshaw, R.A., Gurd, F.R.N. (1969). J. Biol. Chem.244, 2167–2181
Bruce, E.J., Castillo, O., Lehmann, H. (1977). FEBS Lett.78, 113–118
Byles, R.H. (1976). Ann Rev. Anthropol.5, 69–90
Carter, C.W., Kraut, J., Freer, S.T., Alden, R.A., Sieker, L.C., Adam, E., Jensen, L.H. (1972). Proc. Nat. Acad. Sci. U.S.A.69, 3526–3529
Castillo, O., Jones, L.T., Lehmann, H. (1978a). Biochim. Biophys. Acta533, 289–292
Castillo, O., Lehmann, H., Diamond, R. (1978b) FEBS Lett.96, 12–14
Castillo, O., Lehmann, H. (1977). Biochim. Biophys. Acta492, 232–236
Castillo, O., Lehmann, H., Joysey, K.A., Friday, A.E. (1977). In: Myoglobin, A.G. Schneck, C. Vandescasserie, eds., pp. 130–141, Brussels: Editions de l'Université de Bruxelles
Chothia, C. (1974). Nature248, 338–339
Chou, P.Y., Fasman, G.D. (1974). Biochemistry13, 222–245
Clarke, B. (1970a). Nature228, 159–160
Clarke, B. (1970b). Science168, 1009–1011
Crow, J.F. (1972). In: Proceedings of the 6th Berkeley symposium on mathematical statistics and probability, L.M. Leran et al., eds. Vol. 5, Berkeley and Los Angeles: Univ. Calif. Press
Darbre, P.D., Lehmann, H. (1976). Biochim. Biophys. Acta453, 285–288
Darbre, P.D., Romero-Herrera, A.E., Lehmann, H. (1975). Biochim. Biophys. Acta393, 201–204
Dautrevaux, M., Boulanger, Y., Han, K., Biserte, G. (1969). Eur. J. Biochem.11, 267–277
Davies, D.R., Padlan, E.A., Segal, D.M. (1975). Ann. Rev. Biochem.44, 639–667
Dawson, D.M. (1972). In: The biochemical genetics of man, D.J.H. Brock, O. Mayo, eds., New York: Academic Press
Dayhoff, M.O. (1972). Atlas of protein sequence and structure, Silver Spring, MD: Nat. Biomed. Res. Foundation
DiMarchi, R.D., Wang, C.-C., Hemenway, J.B., Gurd, F.R.N. (1978). Biochemistry17, 1968–1970
Dixon, M. (1973). Bio-medical programs, Los Angeles: Health Services Computer Facility, UCLA
Dobzhansky, T. (1962). Mankind evolving: the evolution of the human species, pp. 154–156, Clinton, MA: The Colonial Press, Inc.
Dumur, V., Dautrevaux, M., Han, K. (1976). Biochim. Biophys. Acta420, 376–386
Dwulet, F.E., Bogardt, R.A., Jones, B.N., Lehman, L.D., Gurd, F.R.N. (1975). Biochemistry14, 5336–5343
Dwulet, F.E., Jones, B.N., Lehman, L.D., Gurd, F.R.N. (1977). Biochemistry16, 873–877
Dwulet, J.A., Dwulet, F.E., Gurd, F.R.n. (1980). Biochim. Biophys. Acta (in press)
Epstein, C.J. (1967). Nature215, 355–359
Eventoff, W., Rossman, M.G. (1975). CRC Crit. Rev. Biochem.3, 111–140
Ewens, W.J. (1977). Adv. Hum. Genet.8, 67–101
Fisher, W.K., Thompson, E.O.P. (1976). Aust. J. Biol. Sci.29, 57–65
Fitch, W.M. (1973). Ann. Rev. Genetics7, 343–380
Frauenfelder, H., Petsko, G.A., Tsernoglou, D. (1979). Nature280, 558–563
Friend, S.H. (1979). Ph.D. Thesis, Indiana University
Friend, S.H., Gurd, F.R.N. (1979). Biochemistry18, 4620–4630
Friend, S.H., March, K.L., Hanania, G.I.H., Gurd, F.R.N. (1980) Biochemistry (in press)
Goldsack, D.E., Chalifoux, R.C. (1973). J. Theor. Biol.39, 645–651
Grantham, R. (1974). Science185, 862–864
Gurd, F.R.N., Rothgeb, T.M. (1979). Adv. Protein Chem.33, 73–165
Han, K., Dautrevaux, M., Chaila, X., Biserte, C. (1970). Eur. J. Biochem.16, 465–471
Han, K., Tetaert, D., Moschetta, Y., Dautrevaux, M., Kapeyan, C. (1972). Eur. J. Biochem.27, 585–592
Hutchens, J.O. (1970). In: Handbook of biochemistry. Selected data for molecular biology, 2nd edition, H.A. Sober, ed., p. B-61, Cleveland: The Chemical Rubber Co.
Jones, B.N. (1977). Ph.D. Thesis, Indiana University
Jones, D.D. (1975). J. Theor. Biol.50, 167–183
Jones, B.N., Dwulet, F.E., Lehman, L.D., DiMarchi, R.D., Wang, C.-C., Meuth, J.L., Bogardt, R.A. (1977). Fed. Proc., Fed. Am. Soc. Exp. Biol.36, 754
Jones, B.N., Dwulet, F.E., Lehman, L.D., Garner, M.H., Bogardt, R.A., Garner, W.H., and Gurd, F.R.N. (1978). Biochemistry17, 1971–1974
Jones, B.N., Rothgeb, T.M., England, R.D., Gurd, F.R.N. (1979a). Biochim. Biophys. Acta577, 464–474
Jones, B.N., Vigna, R.A., Dwulet, F.E., Bogardt, R.A., Lehman, L.D., Gurd, F.R.N. (1976). Biochemistry15, 4418–4422
Jones, B.N., Wang, C.-C., Dwulet, F.E., Lehman, L.D., Meuth, J.L., Bogardt, R.A., Gurd, F.R.N. (1979b). Biochim. Biophys. Acta577, 454–463
Jungck, J.R. (1978). J. Mol. Evol.11, 211–224
Kabat, E.A. (1978). Adv. Prot. Chem.32, 1–75
Kimura, M. (1968). Nature217, 624–626
Kimura, M. (1969). Proc. Nat. Acad. Sci. U.S.A.63, 1181–1188
Kimura, M., Ohta, T. (1971). J. Mol. Evol.1, 1–17
Kimura, M., Ohta, T. (1974). Proc. Nat. Acad. Sci. U.S.A.71, 2848–2852
King, J.L., Jukes, T.H. (1969). Science164, 788–798
Lee, B., Richards, F.M. (1971). J. Mol. Biol.55, 379–400
Lehman, L.D., Dwulet, F.E., Bogardt, R.A., Jones, B.N., Gurd, F.R.N. (1977). Biochemistry16, 706–709
Lehman, L.D., Dwulet, F.E., Jones, B.N., Bogardt, R.A., Krueckeburg, S.T., Visscher, R.B., Gurd, F.R.N.(1978). Biochemistry17, 3736–3739
Lehman, L.D., Jones, B.N., Dwulet, F.E., Bogardt, R.A., Gurd, F.R.N. (1980). Biochim. Biophys. Acta (in press)
Lenstra, J.A., Hofsteenge, J., Beintema, J.J. (1977). J. Mol. Biol.109, 185–193
Lewontin, R.C. (1974). The genetic basis of evolutionary change, New York: Columbia University Press
Liquori, A.M., Sadun, C. (1978). Gazz. Chim. Ital.108, 513–517
Love, W.E., Klock, P.A., Lattman, E.E., Padlan, E.A., Ward, K.B., Hendrickson, W.A. (1972). Cold Spring Harbor Symp. Quant. Biol.36, 349–360
McCammon, J.A., Karplus, M. (1980). Crit. Rev. Biochem (in press)
Matthew, J.B., Friend, S.H., Botelho, L.H., Lehman, L.D., Hanania, G.I.H., Gurd, F.R.N. (1978). Biochem. Biophys. Res. Commun.81, 416–421
Matthew, J.B., Hanania, G.I.H., Gurd, F.R.N. (1979) Biochemistry18, 1919–1927
Meuth, J.L., Jones, B.N., Garner, W.H., and Gurd, F.R.N. (1978). Biochemistry17, 3429–3431
Morrison, D.F. (1967). Multivariate statistical methods, New York: McGraw-Hill
Nei, M. (1975). Molecular population genetics and evolution, New York: Elsevier
Nolan, C., Margoliash, E., (1968). Ann. Rev. Biochem.37, 727–790
Nozaki, Y., Tanford, C. (1971). J. Biol. Chem.246, 2211–2217
Padlan, E.A., Love, W.E. (1974). J. Biol. Chem.249, 4067–4078
Perutz, M.F. (1970). Nature228, 726–739
Richards, F.M. (1979). Carlsberg Res. Commun.44, 47–63
Romero-Herrera, A.E., Darbre, P.D., Lehmann, H. (1976a). Biochim. Biophys. Acta420, 350–357
Romero-Herrera, A.E., Lehmann, H. (1971). Biochim. Biophys. Acta251, 482–488
Romero-Herrera, A.E., Lehmann, H. (1972a). Biochim. Biophys. Acta278, 62–67
Romero-Herrera, A.E., Lehmann, H. (1972b). Biochim. Biophys. Acta278, 465–481
Romero-Herrera, A.E., Lehmann, H. (1973a). Biochim. Biophys. Acta317, 65–84
Romero-Herrera, A.E., Lehmann, H. (1973b). Biochim. Biophys. Acta322, 10–22
Romero-Herrera, A.E., Lehmann, H. (1974a). Biochim. Biophys. Acta359, 236–241
Romero-Herrera, A.E., Lehmann, H. (1974b). Proc. R. Soc. London, Ser. B.186, 249–279
Romero-Herrera, A.E., Lehmann, H. (1974c). Biochim. Biophys. Acta336, 318–323
Romero-Herrera, A.E., Lehmann, H. (1975a). Biochim. Biophys. Acta393, 205–214
Romero-Herrera, A.E., Lehmann, H. (1975b). Biochim. Biophys. Acta400, 387–398
Romero-Herrera, A.E., Lehmann, H., Castillo, O. (1976b). Biochim. Biophys. Acta420, 387–396
Romero-Herrera, A.E., Lehmann, H., Castillo, O. (1976c). Biochim. Biophys. Acta439, 51–54
Romero-Herrera, A.E., Lehman, H., Castillo, O., Joysey, K.A., Friday, A.E. (1976d). Nature261, 162–164
Romero-Herrera, A.E., Lehman, L.D., Fukes, W. (1975a). Biochim. Biophys. Acta379, 13–21
Romero-Herrera, A.E., Lehmann, H., Fossey, D. (1975b). Biochim. Biophys. Acta393, 383–388
Rossmann, M.G., Argos, P. (1978). Molec. Cellu. Biochem.21, 131–182
Rothgeb, T.M., and Gurd, F.R.N. (1978). In: Methods in enzymology. Fleischer, S., Packer, L., eds. Vol. 52, Part C, p. 473–486, New York: Academic Press
Rousseaux, J., Dautrevaux, M., Han, K. (1976). Biochim. Biophys. Acta439, 55–62
Salemme, F.R. (1977). Ann. Rev. Biochem.46, 299–329
Scouloudi, H. (1978). J. Mol. Biol.126, 661–671
Scouloudi, H., Baker, E.N. (1978). J. Mol. Biol.126, 637–660
Segal, I.H. (1968). Biochemical calculations, p. 3, New York: John Wiley
Shire, S.J., Hanania, G.I.H., Gurd, F.R.N. (1974). Biochemistry13, 2967–2974
Sneath, P.H.A. (1966). J. Theor. Biol.12, 157–195
Sokal, R.R., Rohlf, F.J. (1969). Biometry, the principles and practice of statistics in biological research, San Francisco: W.H. Freeman
Stamatoyannopoulos, G. (1972). Ann. Rev. Genetics6, 47–89
Takano, T. (1977). J. Mol. Biol.110, 537–568
Tetaert, D., Han, K., Planeot, M., Dautrevaux, M., Ducastaing, S., Hombrados, 1., Neuzil, E. (1974). Biochim. Biophys. Acta351, 317–324
Vainshtein, B.K., Arutyunyah, E.G., Kuranova, I.P., Barisov, V.V., Sosfenov, N.I., Pavlovskii, A.G., Grebenko, A.I., Konareva, N.V. (1974). Dokl. Akad. Nauk. S.S.S.R.216, 690–701
Vigna, R.A., Gurd, L.J., and Gurd, F.R.N. (1974). J. Biol. Chem.249, 4144–4148
Visscher, R.B., Gurd, F.R.N. (1975). J. Biol. Chem.250, 2238–2242
Vötsch, W., Anderer, F.A. (1975). J. Mol. Evol.5, 315–321
Wang, C.-C., Avila, R., Jones, B.N., Gurd, F.R.N. (1977). Biochemistry16, 4978–4981
Watson, H.C. (1969). Prog. Stereochem.4, 299–333
Waugh, D.F. (1954). Adv. Prot. Chem.9, 344–437
Weeds, A.G., McLachlan, A.D. (1974). Nature252, 646–649
Wilson, A.C., Carlson, S.S., White, T.J. (1977). Ann. Rev. Biochem.46, 573–640
Wittebort, R.J., Rothgeb, T.M., Szabo, A., Gurd, F.R.N. (1979) Proc. Natl. Acad. Sci. USA76, 1059–1063
Woese, C.R. (1973). Naturwissenschaften60, 447–459
Wu, T.T., Fitch, W.M., Margoliash, E. (1974). Ann. Rev. Biochem.43, 539–566
Zimmerman, J.M., Eliezer, N., Simha, R. (1968). J. Theor. Biol.21, 170–201
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Bogardt, R.A., Jones, B.N., Dwulet, F.E. et al. Evolution of the amino acid substitution in the mammalian myoglobin gene. J Mol Evol 15, 197–218 (1980). https://doi.org/10.1007/BF01732948
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DOI: https://doi.org/10.1007/BF01732948