Summary
Soluble lead salts and a number of lead-containing minerals catalyze the formation of oligonucleotides from nucleoside 5′-phosphorimidazolides. The effectiveness of lead compounds correlates strongly with their solubility. Under optimal conditions we were able to obtain 18% of pentamer and higher oligomers from ImpA. Reactions involving ImpU gave smaller yields.
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Abbreviations
- A:
-
adenosine
- U :
-
uridine
- Im :
-
imidazole
- MeIm :
-
1-methyl-imidazole
- EDTA :
-
ethylenediaminetetraacetic acid
- pA :
-
adenosine 5′-phosphate
- pU :
-
uridine 5′-phosphate
- Ap :
-
adenosine cyclic 2′:3′-phosphate
- ATP :
-
adenosine 5′-triphosphate
- AppA :
-
P1,P2-diadenosine 5′-diphosphate
- pNp (N = A,U) :
-
nucleotide 2′(3′), 5′-diphosphate
- ImpA :
-
adenosine 5′-phosphoreimidazolide
- ImpU :
-
uridine 5′-phosphorimidazolide
- A 2pA:
-
adenylyl-[2′→5′]-adenosine
- A 3pA:
-
adenylyl-[3′→5′]-adenosine
- pA 2pA:
-
5′-phospho-adenylyl-[2′→5′]-adenosine
- pA 3pA:
-
5′-phospho-adenylyl-[3′→5′]-adenosine
- pUpU :
-
5′-phospho-uridylyl-uridine
- pApU :
-
5′-phospho-adenylyl-uridine
- pUpA :
-
5′-phospho-uridylyladenine
- (pA)n (n, 2,3,4,⋯) :
-
oligoadenylates with 5′ terminal phosphate
- ImpApA :
-
5′-phosphorimidazolide of adenylyl adenosine
- (pA) 5+ :
-
pentamer and higher oligoadenylates with 5′ terminal phosphate
- (Ap)nA (n = 2,3,4⋯) :
-
oligoadenylates without terminal phosphates
References
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In the following we do not specify the nature of the internucleotide linkage
In the following we do not specify the nature of the internucleotide linkage
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Sleeper, H.L., Orgel, L.E. The catalysis of nucleotide polymerization by compounds of divalent lead. J Mol Evol 12, 357–364 (1979). https://doi.org/10.1007/BF01732030
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DOI: https://doi.org/10.1007/BF01732030