Summary
Hybrid strains ofEscherichia coli andSalmonella typhimurium were constructed in which intergeneric complementation was required for tryptophan biosynthesis. The functional aspects of in vivo tryptophan biosynthesis in these hybrid strains were compared with similar strains ofE. coli. There was little indication that the hybrid strains were deficient in tryptophan biosynthesis as a result of their hybrid nature, although the tryptophan operons of the two species differ by approximately one thousand mutations. It is tentatively concluded that this genetic divergence has not detectably altered the functional properties of the operon and is likely to be the result of accumulation of neutral mutations.
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Abbreviations
- PRA:
-
N-(5′ phophoribosyl)anthranilic acid
- CdRP:
-
1-(o-carboxyphenylamino)-1-deoxyribulose phosphate
- InGP:
-
indole-3-glycerol phosphate
- InG(P):
-
InGP or its dephosphorylated form
References
Allen, M.A., Yanofsky, C. (1963). Genet. 48, 1065
Arnheim, N., Taylor, C.E. (1969). Nature 223, 900
Balbinder, E. (1964). Biochem. Biophys. Res. Commun. 17, 770
Balbinder, E., Blume, A.J., Weber, A., Tamaki, H. (1968). J. Bact. 95, 2217
Balbinder, E., Callahan, R., McCann, P.P., Cordaro, J.C., Weber, A.R., Smith, A.M., Angelosanto, F. (1970). Genet. 66, 31
Baron, L.S., Gemski, P., Johnson, E.M., Wohlhieter, J.A. (1968). Bact.Revs. 32, 362
Bauerle, R.H., Margolin, P. (1966). Cold Spring Harbor Symp. Quant. Biol. 31, 203
Bauerle, R.H., Margolin, P. (1967). J.Mol.Biol. 26, 423
Blume, A.J., Balbinder, E. (1966). Genet. 53, 577
Breed, R.S., Murray, E.G.D., Smith, N.R. (1959). Bergey's manual of determinative bacteriology, 7th edition. Baltimore: Williams and Wilkins
Brenner, D.J., Falkow, S. (1966). Adv. Genet. 53, 577
Clarke, B. (1970). Sci. 168, 1009
Cordaro, J.C., Balbinder, E. (1971). Genet. 16, 81
Cox, E.C., Yanofsky, C. (1967). Proc. Nat. Acad. Sci. (Wash.) 58, 1895
Crawford, I.P., Johnson, L.M. (1964). Genet. 49, 267
Creighton, T.E. (1968). J. Biol. Chem. 243, 5605
Creighton, T.E. (1970a). Biochem. J. 120, 699
Creighton, T.E. (1970b). Eur. J. Biochem. 13, 1
Creighton, T.E., Helinski, D.R., Somerville, R.L., Yanofsky, C. (1966). J. Bact. 91, 1819
Creighton, T.E., Yanofsky, C. (1966). J. Biol. Chem. 241, 980
Creighton, T.E., Yanofsky, C. (1970). Methods Enzymol. 17A, 365
Denney, R.M., Yanofsky, C. (1972). J. Mol. Biol. 64, 319
Eisenstein, R.B., Yanofsky, C. (1962). J. Bact. 83, 193
Faeder, E.J., Hammes, G.G. (1970). Biochem. 9, 4043
Fredericq, P. (1963). Proc. 11th Intl. Congr. Genet. 1, 42
Gibson, T.C., Scheppe, M.L., Cox, E.C. (1970). Sci. 169, 686
Ino, I., Demerec, M. (1968). Genet. 59, 167
Ito, J. (1969). Nature 223, 57
Ito, J., Crawford, I.P. (1965). Genet. 52, 1303
Ito, J., Yanofsky, C. (1966). J. Biol. Chem. 241, 4112
Ito, J., Yanofsky, C. (1969). J. Bact. 97, 734 (1969)
Ito, K., Hiraga, H., Yura, T. (1969). Genet. 61, 521
Jacobs, E.E., Sanadi, D.R. (1960). J. Biol. Chem. 235, 531
Kimura, M. (1968). Nature 217, 624
King, J.L., Jukes, T.H. (1969). Sci. 164, 788
Li, S., Denney, R.M., Yanofsky, C. (1973). Proc. Nat. Acad. Sci. (Wash.) 70, 1112
Li, S., Yanofsky, C. (1972). J. Biol. Chem. 247, 1031
Li, S., Yanofsky, C. (1973a). J. Biol. Chem. 248, 1830
Li, S., Yanofsky, C. (1973b). J. Biol. Chem. 248, 1837
Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). J. Biol. Chem. 193, 265
McQuade, J.F., Creighton, T.E. (1970). Eur. J. Biochem. 16, 199
Margoliash, E. (1973). Harvey Lectures 66, 177
Mattoon, J.R., Sherman, F. (1966). J. Biol. Chem. 241, 4330
Middleton, R.B., Mojica-a, T. (1971). Adv. Genet. 16, 53
Miyake, T., Demerec, M. (1959). Nature 183, 1586
Mojica-a, T., Middleton, R.B. (1972). Genet. 71, 491
Morse, D.E., Yanofsky, C. (1968). J. Mol. Biol. 38, 447
Moyed, H.S. (1960). J. Biol. Chem. 235, 1098
Murphy, T.M., Mills, S.E. (1969). J. Bact. 97, 1310
Richmond, R. C. (1970). Nature 225, 1025
Roberts, R.B., Abelson, P.H., Cowie, D.B., Bolton, E.T., Britten, R.J. (1957). Studies of biosynthesis inEscherichia coli, p. 313. Washington, D.C.: Carnegie Institute of Washington
Sanderson, K.E. (1971). Adv. Genet. 16, 35
Sanderson, K.E., Hall, C.A. (1970). Genet. 64, 215
Signer, E.R. (1965). J. Mol. Biol. 12, 7
Smith, O.H. (1967). Genet. 57, 95
Somerville, R.L. (1966). Sci. 154, 1585
Somerville, R.L., Yanofsky, C. (1965). J. Mol. Biol. 11, 747
Stetson, H., Somerville, R.L. (1971). Mol.Gen. Genet. 111, 342
Sullivan, B. (1972). J. Mol. Evol. 1, 295
Vogel, H.J., Bonner, D.M. (1956). J. Biol. Chem. 218, 97
Wuesthof, G., Bauerle, R.H. (1970). J. Mol. Biol. 49, 171
Yanofsky, C. (1956). J. Biol. Chem. 273, 171
Yanofsky, C. (1971). J. Amer. med. Assoc. 218, 1026
Yanofsky, C., Crawford, I.P. (1959). Proc. Nat. Acad. Sci. (Wash.) 45, 1016
Yanofsky, C., Crawford, I.P. (1972). In: The enzymes, Vol. 7, P. Boyer, ed., p. 1. New York: Academic Press
Zamenhof, S., Eichhorn, H.H. (1967). Nature 216, 456
Zinder, N.D. (1965). Sci. 131, 813
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Creighton, T.E. The functional significance of the evolutionary divergence between the tryptophan operons ofEscherichia coli andSalmonella typhimurium . J Mol Evol 4, 121–137 (1974). https://doi.org/10.1007/BF01732018
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DOI: https://doi.org/10.1007/BF01732018