Abstract
The phenol emulsion reassociation technique was used to isolate and clone a female specific, repetitive DNA sequence fromLarus fuscus. The repeat, designated P2000-17, is restricted to the W chromosome, although related sequences occur elsewhere in the genome ofL. fuscus. Similar sequences were detected in the genome of six other bird species from outside the genus Laridae, but the sequence occurs less frequently and to a similar extent in both sexes. The 298 bp DNA sequence of P2000-17 was determined and found to have extensive sequence identity to the rabbit dihydropyridine (DHP) receptor calcium channel. P2000-17 is represented once within a larger 8.6 kb tandem repeat (LfW-1), which has a complex internal DNA sequence. LfW-1 is highly conserved between repeat motifs and may comprise 3% of the female genome. The possible evolutionary origin of LfW-1 is discussed in relation to the repeat types found on the W and Y chromosomes of other species.
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Ansari HA, Takagi N, Sasaki M (1988) Morphological differentiation of sex chromosomes in three species of ratite birds. Cytogenet Cell Genet 47:185–188
Baumann A, Grupc A, Ackermann A, Pongs O (1988) Structure of the voltage-dependent potassium channel is highly conserved from Drosophila to vertebrate central nervous systems. EMBO J 7:2457–2463
Bishop CE, Boursot B, Bonhomme F, Hatat D (1985) Most classicalMus musuclus domesticus laboratory mouse strains carry aMus musculus musculus Y chromosome. Nature 315:70–72
Bull JJ (1983) Evolution of sex determining mechanisms. Benjamin/Cummings, Menlo Park, Calif
Campbell KP, Lcung AT, Sharp AII (1988) The biochemistry and molecular biology of the dihydropyridine-sensitive calcium channel. Trends Neurol Sci 11:425–430
Charlesworth B (1978) Model for the evolution of Y chromosomes and dosage compensation. Proc Natl Acad Sci USA 75:5618–5622
Cooke HJ (1976) Repeated sequence specific to human males. Nature 262:182–186
Cooke HJ, Schmidke J, Gosden JR (1982) Characterization of a human Y chromosome repeated sequence and related sequences in higher primates. Chromosoma 87:491–502
Cooke HJ, Brown WAR, Rappold GA (1984) Closely related sequences on human X and Y chromosomes outside the pairing region. Nature 311:259–261
Costanzi C, Gillespie D (1987) Fast blot immobilization of DNA and RNA from cells. Methods in Enzymol 152:582–588
Coulson JC, Thomas CS, Butterfield JEL, Duncan N, Monaghan P, Sledder C (1983) The use of head and bill length to sex live gulls, Laridae. Ibis 125:549–557
Deininger PL, Daniels GR (1986) The recent evolution of mammalian repetitive DNA elements. Trends Genet 2:76–80
Dootlittle WF, Sapienza C (1980) Selfish genes, the phenotype paradigm and genome evolution. Nature 284:601–603
Eicher EM, Hutchinson KW, Philips SJ, Tucker PK, Lee BK (1989) A repeated segment on the mouse Y chromosome is composed of retroviral-related, Y enriched and Y specific sequences. Genetics 122:181–192
Ellis SB, Williams ME, Ways NR, Brenner R, Sharp AH, Leung AT, Campbell KP, McKenna E, Koch WJ, Hui A, Schwartz A, Harpold MM (1988) Sequence and expression of mRNA's encoding the 1 and 2 subunits of a DHP-sensitive calcium channel. Science 241:1661–1664
Feinberg AP, Vogelstein B (1984) Addendum: a technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–227
Fickett JW (1982) Recognition of protein coding regions in a DNA sequence. Nucleic Acids Res 10:5303–5318
Finnegan DJ (1989) Eukaryotic transposable elements and genome evolution. Trends Genet 5:103–107
Hamilton WD (1967) Extraordinary sex ratios. Science 156:477–488
Hanahan D (1985) Techniques for transformation ofE. coli. In: Glover DM (ed) DNA cloning vol I: a practical approach. IRL press Oxford, p 109–135
John B, Miklos BLB (1979) Functional aspects of satellite DNA and heterochromatin. Int Rev Cytol 58:1–23
Kodama H, Saitoh H, Tone M, Kuhara S, Sasaki Y, Mizuno S (1987) Nucleotide sequences and unusual electrophoretic behavior of the W chromosome-specific repeating DNA units of the domestic fowl, Gallus gallus domesticus. Chromosoma 96:18–25
Kohne DF, Levinson SA, Byers MJ (1977) Room temperature method for increasing the rate of DNA reassociation by many thousand fold: the phenol emulsion reassociation technique. Biochemistry 16:5329–5341
Kunkel LM, Smith KD (1982) Evolution of human Y chromosome DNA. Chromosoma 86:209–228
Kunkel LM, Monaco AP, Middlesworth W, Ochs HD, Latts SA (1985) Specific cloning of DNA fragments absent from the DNA of a male patient with an X chromosome deletion. Proc Natl Acad Sci USA 82:4778–4782
Lamar EE, Palmer E (1984) Y-encoded, species specific DNA in mice: evidence that the Y chromosome exists in two polymorphic forms in inbred strains. Cell 37:171–177
Levinson G, Marsh JL, Epplen JT, Gutman GA (1985) Cross hybridizing snake minisatellite,Drosophila and mouse DNA sequences may have arisen independently. Mol Biol Evol 2:494–504
Lipman DJ, Pearson WR (1985) Rapid and sensitive protein similarity searches. Science 227:1435–1441
Maniatis T, Fritsch EF, Sambrook KJ (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbour, NY
Muller HJ (1914) A gene for the fourth chromosome ofDrosophila. J Exp Zool 17:324–337
Muller HJ (1918) Genetic variability, twin hybrids and constant hybrids, in a case of balanced lethan factors. Genetics 3:422–499
Ohno S (1967) Sex chromosomes and sex linked genes. Springer, Berlin Heidelberg New York
Orgel LE, Crick FH (1980) Selfish DNA: the ultimate parasite. Nature 284:604–607
Page DC, Harper ME, Love J, Botstein D (1984) Occurrence of a transposition from the X-chromosome long arm to the Ychromosome short arm during human evolution. Nature 311:119–123
Ramaswami M, Tanouye MA (1989) Two sodium-channel genes inDrosophila: implications for channel diversity. Proc Natl Acad Sci USA 86:2079–2082
Ray-Chaudhuri SP, Singh L, Sharma T (1970) Sexual dimorphism in somatic interphase nuclei of snakes. Cytogenetics 91:140–423
Ray-Chaudhuri SP, Singh L, Sharma T (1971) Evolution of sex chromosomes and formation of W chromatin in snakes. Chromosoma 33:239–251
Rice WR (1987a) Genetic hitchhiking and the evolution of reduced genetic activity of the Y sex chromosome. Genetics 116:161–167
Rice WR (1987b) The accumulation of sexually antagonistic genes as a selective agent promoting the evolution of reduced recombination between primitive sex chromosomes. Evolution 41:911–914
Serghini MA, Ritzenthaler C, Pinck L (1989) A rapid and efficient ‘miniprep’ for the isolation of plasmid DNA. Nucleic Acids Res 17:3604
Shepherd JCW (1981) Method to determine the reading frame of a protein from the purine/pyrimidine genome sequence and its possible evolutionary justification. Proc Natl Acad Sci USA 78:1596–1600
Singh L, Purdom IF, Jones KW (1976) Satellite DNA and evolution of sex chromosomes. Chromosoma 59:43–62
Singh L, Purdom IF, Jones KW (1980) Sex chromosome associated satellite DNA: evolution and conservation. Chromosoma 79:137–157
Singh L, Purdom IF, Jones KW (1981) Conserved sex chromosome associated nucleotide sequences in eukaryotes. Cold Spring Harbor Symp Quant Biol 45:805–814
Smith GP (1976) Evolution of repeated DNA sequences by unequal crossover. Science 191:528–535
Staden R (1984a) Graphic methods to determine the function of nucleic acid sequences. Nucleic Acids Res 12:521–538
Staden R (1984b) Measurement of the effects that coding for a protein has on a DNA sequence and their use for finding genes. Nucleic Acids Res 12:551–567
Steele PE, Rabson AB, Bryan T, O'Brien SJ (1986) Amplification and chromosomal dispersion of human endogenous retroviral sequences. J Virol 59:545–550
Stefos K, Arrighi FE (1971) The heterochromatic nature of the W chromosome in birds. Exp Cell Res 68:228–231
Stefos K, Arrighi FE (1974) Repetitive DNA of Gallus domesticus and its cytological locations. Exp Cell Res 83:9–14
Su TS, Nussbaum RL, Airhart S, Ledbetter DH, Mohandas T, O'Brien WE, Beaudet AL (1984) Human chromosomal assignments for 14 argenininosuccinate synthetase pseudogenes: cloned DNA's as reagents for cytogenetic analysis. Am J Hum Genet 36:954–964
Tanabe T, Takeshima H, Mikami A, Flockerzi V, Takahashi H, Kangowa K, Kojima M, Matsuo H, Hirose T, Numa S (1987) Primary structure of the receptor for calcium channel blockers from skeletal muscle. Nature 328:313–328
Tinbergen N (1960) The herring gull's world. Harper and Row, Boston
Tone M, Nakano N, Takao E, Narisawa S, Mizuno S (1982) Demonstration of W chromosome-specific repetitive DNA sequences in the domestic fowlGallus g. domesticus. Chromosoma 86:551–569
Tone M, Sakaki Y, Hashiguchi T, Mizuno S (1984) Genus specificity and extensive methylation of the W chromosome-specific repetitive DNA sequences from the domestic fowl,Gallus gallus domesticus. Chromosoma 89:228–237
Vanin EF (1985) Processed pseudogenes: characteristics and evolution. Annu Rev Genet 19:253–272
Venturini G, Capanna E, Fontana B (1987) Size and structure of the bird genome II. Repetitive DNA and sequence organization. Comp Biochem Physiol 87B:975–979
Wagner M (1986) A consideration of the origin of processed pseudogenes. Trends Genet 2:134–137
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Griffiths, R., Holland, P.W.H. A novel avian W chromosome DNA repeat sequence in the lesser black-backed gull (Larus fuscus). Chromosoma 99, 243–250 (1990). https://doi.org/10.1007/BF01731699
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DOI: https://doi.org/10.1007/BF01731699