Abstract
A mechanism for respiration and oxidative phosphorylation at Site I is proposed which involves reduction by NADH of a thioester of a fatty acid to aldehyde. Oxidation of the aldehyde by non-heme iron forms fatty acid which initially binds to a membrane base. As the non-heme iron reduces, entropy is lost through the stretching of a lipid bilayer attached to the non-heme iron and the membrane and to which the fatty acid chain binds. Simultaneously energy is expended in separating carboxyl ion from the protonated base. The charge separation induces movements of protons and reactants which result in the formation of ATP. Subsequent oxidation of non-heme iron relaxes the stretched lipid and the entropy gain in the fatty acid contributes to reformation of its thioester. The mechanism accounts in detail for many observations.
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References
D. E. Weiss,Aust. J. Biol. Sci 22 (1969) 1389.
D. E. Weiss,Search,2 (1971) 48.
D. E. Weiss, Mary L. Webb, F. L. Bygrave and R. N. Robertson, Unpublished data.
E. C. Slater,Nature,172 (1953) 975.
P. D. Boyer, in:Oxidases and Related Redox Systems, T. E. King, H. S. Mason and M. Morrison (eds.), John Wiley and Sons, Inc, New York, Vol. 2, 1964, p. 1944.
B. T. Storey,J. Theor. Biol. 28 (1970) 233.
R. A. Harris, J. T. Penniston, J. Asai and D. E. Green,Proc. Nat. Acad. Sci., U.S.A.,59 (1968) 830.
T. E. King, M. Kuboyama and S. Takemori, in:Oxidases and Related Redox Systems, T. E. King, H. S. Mason and M. Morrison (eds.) John Wiley and Sons, Inc, New York, Vol. 2, 1964, p. 707.
G. D. Greville, in:Current Topics in Bioenergetics, D. R. Sanadi (ed.),3 (1969) 1.
R. J. P. Williams,Current Topics in Bioenergetics,3 (1969) 79.
P. D. Boyer, in:Biological Oxidation, T. P. Singer (ed.), Interscience, 1967, p. 193.
D. E. Weiss,Aust. J. Biol. Sci.,22 (1969) 1337.
D. E. Weiss,Aust. J. Biol. Sci.,22 (1969) 1355.
D. E. Weiss,Aust. J. Biol. Sci.,22 (1969) 1373.
M. Klingenberg, R. Wulf, H. W. Heldt and E. Pfaff,FEBS Symposium,17 (1969) 59.
J. M. Lowenstein and B. Chance,J. Biol. Chem.,243 (1968) 3940.
K. van Dam and E. C. Slater,Proc. Nat. Acad. Sci., U.S.A.,58 (1967) 2015.
V. P. Skulachev, A. A. Jasaites, V. V. Navickaite, L. S. Yaguzhinsky, E. A. Liberman, V. P. Topali and L. M. Zogina,FEBS Symposium,17 (1969) 275.
M. J. Weidemann, H. Erdelt and M. Klingenberg,Eur. J. Biochem.,16 (1970) 313.
D. F. Wilson, H. P. Ting and M. S. Koppelman,Biochem.,10 (1971) 2897.
C. I. Ragan and P. B. Garland,Biochem. J.,124 (1971) 171.
B. Chance, C. P. Lee, I. Y. Lee, T. Ohnishi and J. Higgins, in:Electron Transport and Energy Conservation, J. M. Tager, S. Papa, E. Quagliariello and E. C. Slater (eds.), Adriatica Editrice, 1970, p. 29.
T. P. Singer and M. Gutman,Advances in Enzymol.,34 (1971) 79.
L. Wojtczak, K. Bogucka, M. G. Sarzala and H. Zaluska,FEBS Symposium,17 (1969) 79.
S. G. Van den Bergh, C. P. Modder, J. H. M. Souverijn and H. C. J. M. Pierrot,FEBS Symposium,17 (1969) 137.
A. B. Falcone and R. L. Mao,Biochim. Biophys. Acta,105 (1965) 233.
A. B. Falcone and R. L. Mao,Biochim. Biophys. Acta,105 (1965) 246.
B. Mackler, M. L. Cowger and R. F. Igo,Biochim. Biophys. Acta,52 (1961) 203.
K. V. Rajagopalan and P. Handler,J. Biol. Chem. 239 (1964) 2022, 2027.
S. P. J. Albracht and E. C. Slater,Biochim. Biophys. Acta,223 (1970) 457.
M. Gutman, M. Mayr, R. Oltzik and T. P. Singer,Biochem. Biophys. Res. Commun.,41 (1970) 40.
C. S. Yang and F. M. Huennekens,Biochem.,9 (1970) 2127.
C. R. Hackenbrock,J. Cell. Biol.,37 (1968) 345.
T. E. King and F. C. Yong, in:Electron Transport and Energy Conservation, J. M. Tager, S. Papa, E. Quagliariello and E. C. Slater (eds.), Adriatica Editrice, Bari, 1970, p. 108.
S. Fleischer, A. Casu and B. Fleischer,Fed. Proc.,23, (1964) 486.
J. Machinist and T. P. Singer,J. Biol. Chem.,240 (1965) 3182.
M. Gutman, T. P. Singer, H. Beinert and J. E. Casida,Proc. Nat. Acad. Sci.,65 (1970) 763.
D. J. Horgan, M. Ohno, T. P. Singer and J. E. Casida,J. Biol. Chem.,243 (1968) 5967.
P. Björntorp, H. A. Ellis and R. H. Bradford,J. Biol. Chem.,239 (1964) 339.
H. W. Heldt and M. Klingenberg,Europ. J. Biochem.,4 (1968) 1.
J. M. Lyons and J. K. Raison,Plant Physiol.,45 (1970) 386.
J. K. Raison, J. M. Lyons, R. J. Mehlhorn and A. D. Keith,J. Biol. Chem.,246 (1971) 4036.
R. H. Aranow and L. Witten,J. Phys. Chem.,64 (1960) 1643;J. Chem. Phys.,43 (1965) 1436.
P. M. Seeman,Int. Rev. Neurobiology,9 (1966) 145.
R. A. Harris, J. Munroe, B. Farmer, K. C. Kim and P. Jenkins,Arch. Biochem. Biophys.,142 (1971) 435.
M. Poe, H. Gutfreund and R. W. Estabrook,Arch. Biochem. Biophys.,122 (1967) 204.
D. Devault,Biochim. Biophys. Acta,225 (1971) 193.
M. Gutman, H. Mersmann, J. Lutky and T. P. SingerBiochem.,9 (1970) 2678.
D. D. Tyler, R. A. Butow, J. Gouze and R. W. Estabrook,Biochem. Biophys. Res. Commun.,19 (1965) 551.
L. L. Ingraham, in:Biochemical Mechanisms, 1962, p. 89.
R. J. Van de Stadt, F. J. R. M. Nieuwenhius and K. Van Dam,Biochim. Biophys. Acta,234 (1971) 173.
S. Papa, A. Alifano, J. M. Tager and E. Quagliariello,Biochim. Biophys. Acta,153 (1968) 303.
M. J. Selwyn,Nature,219 (1968) 490.
J. H. M. Souverijn, P. J. Weijers, G. S. P. Groot and A. Kemp,Biochim. Biophys. Acta,223 (1970) 31.
F. L. Bygrave and A. L. Lehninger,Proc. Nat. Acad. Sci. U.S.A.,57 (1967) 1409.
L. L. Grinius, T. I. Guds and V. P. Skulachev,J. Bioenergetics,2 (1971) 101.
L. Packer, M. P. Donovan and J. M. Wrigglesworth,Biochem. Biophys. Res. Commun.,35 (1969) 832.
A. Azzi and H. Vainio, in:Electron Transport and Energy Conservation, J. M. Tager, S. Papa, E. Quagliariello and E. C. Slater (eds.), Adriatica Editrice, Bari, 1970, p. 540.
B. Chance, G. Radda and C. P. Lee, in:Electron Transport and Energy Conservation, J. M. Tager, S. Papa, E. Quagliariello and E. C. Slater (eds.), Adriatica Editrice, Bari, 1970 p. 551.
D. D. Tyler,Biochem. J.,111 (1969) 665.
M. Klingenberg and E. Pfaff, in:Metabolic Roles of Citrate., T. W. Goodwin (ed.), Academic Press, New York, 1968, p. 105.
C. P. Lee and L. Ernster,Eur. J. Biochem.,3 (1968) 301.
B. C. Pressman,J. Biol. Chem.,238 (1963) 401.
D. M. Blow, J. J. Birktoft and B. S. Hartley,Nature,221 (1969) 339.
W. N. Aldridge and M. S. Rose,FEBS Letters,4 (1969) 61.
T. E. Conover, J. Gouze and R. W. Estabrook,Biochim. Biophys. Acta,81 (1964) 587.
R. L. Cross, B. A. Cross and Jui H. Wang,Biochem. Biophys. Res. Commun.,40 (1970) 1155.
R. L. Cross and P. D. Boyer,Fed. Proc.,30(3), Part II, Abstract No. 1127 (1971).
P. D. Boyer,Science,141 (1963) 1147.
P. D. Boyer,Current Topics in Bioenergetics,2, (1967) 99.
R. D. Hill and P. D. Boyer,J. Biol. Chem.,242, (1967) 4320.
R. A. Mitchell, R. D. Hill and P. D. Boyer,J. Biol. Chem.,242 (1967) 1793.
D. M. Jones and P. D. Boyer,J. Biol. Chem.,244 (1969) 5767.
L. Packer and K. Utsumi,Arch. Biochem. Biophys.,131 (1969) 386.
R. A. Dilley,Arch. Biochem. Biophys.,137 (1970) 270.
H. C. Hemker,Biochim. Biophys. Acta,81 (1964) 1 and 9.
R. Amons, S. G. Van den Bergh and E. C. Slater,Biochim. Biophys. Acta,162 (1968) 452.
R. Berezney, Y. C. Awasthi, L. K. Funk and F. L. Crane,J. Bioenergetics,1 (1970) 445.
R. Berezney, Y. C. Awasthi and F. L. Crane,J. Bioenergetics,1 (1970) 457.
A. M. Robertson, C. T. Holloway, I. G. Knight and R. B. Beechey,Biochem. J.,108 (1968) 445.
R. B. Beechey, A. M. Robertson, C. T. Holloway and I. G. Knight,Biochem.,6 (1967) 3867.
K. J. Cattell, I. G. Knight, C. R. Lindop and R. B. Beechey,Biochem. J.,117 (1970) 1011.
A. Bruni, A. Pitotti, A. R. Contessa and P. PalatiniBiochem. Biophys. Res. Commun.,44 (1971) 268.
G. Schafer,Biochim. Biophys. Acta,172 (1969) 334.
R. C. Guillory and E. c. Slater,Biochim. Biophys. Acta,105 (1965) 221.
F. Davidoff,J. Biol. Chem.,246 (1971) 4017.
R. B. Beechey,Biochem. J.,116 (1970) 6P.
R. S. Cockrell, E. J. Harris and B. C. Pressman,Biochem.,5 (1966) 2326.
A. L. Lehninger,Biochem. J.,119 (1970) 129.
B. Chance,J. Biol. Chem.,240 (1965) 2729.
F. Palmieri and E. Quagliariello,Europ. J. Biochem.,8 (1969) 473.
P. B. Garland, B. A. Haddock and D. W. Yates,FEBS Symposium,17 (1969) 111.
S. M. Johnson and A. D. Bangham,Biochim. Biophys. Acta,193 (1969) 92.
A. Kappas and R. H. Palmer,Pharmacol. Rev.,15 (1963) 123.
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Weiss, D.E. Mitochondrial oxidative phosphorylation at Site I involving a fatty aldehyde/acid couple. J Bioenerg Biomembr 3, 305–337 (1972). https://doi.org/10.1007/BF01515976
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DOI: https://doi.org/10.1007/BF01515976