Abstract
The effect of octylguanidine on the Na+ stimulated oxygen uptake of rat liver mitochondria and bovine heart submitochondrial particles and on the Na+ induced efflux of K from the mitochondria has been examined. The results indicate that the action of Na+ is inhibited by octylguanidine, but that the degree of inhibition depends on the concentration of the cation. Apparently, a competition exists between Na+ and octylguanidine for a common site. Octylguanidine, but not oligomycin, at certain concentrations restores in mitochondria incubated with Na+ the capacity to respond to uncouplers. A competitive effect between monovalent cations and octylguanidine has been observed in submitochondrial particles oxidizing NADH.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
A. Gómez-Puyou, F. Sandoval, M. Tuena, A. Peña and E. Chávez,J. Biol. Chem.,244 (1969) 5339.
F. Sandoval, A. Gómez-Puyou, M. Tuena, E. Chávez and A. Peña,Biochemistry,9 (1970) 684.
B. C. Pressman,J. Biol. Chem.,238 (1963) 401.
B. Chance and G. Hollunger,J. Biol. Chem.,238 (1963) 432.
A. Gómez-Puyou, F. Sandoval, A. Peña, E. Chávez and M. Tuena,Biochem. Biophys. Res. Commun.,36 (1969) 316.
A. Gómez-Puyou, F. Sandoval, E. Chávez and M. Tuena,J. Biol. Chem.,245 (1970) 5239.
E. Pinto, A. Gómez-Puyou, F. Sandoval, E. Chávez and M. Tuena,Biochim. Biophys. Acta,223 (1970) 436.
G. Hollunger,Acta Pharmacol. Toxicol., Suppl.1 (1955) 11.
D. W. Haas,Biochim. Biophys. Acta,92 (1964) 433.
R. J. Guillory and E. C. Slater,Biochim. Biophys. Acta,105 (1965) 221.
W. C. Schneider and G. H. Hogeboom,J. Biol. Chem,183 (1950) 123.
C. P. Lee, G. F. Azzone and L. Ernster,Nature,201 (1964) 152.
H. Löw and I. Vallin,Biochim. Biophys. Acta,69 (1963) 361.
C. T. Setllemire, G. R. Hunter and G. P. Brierley,Biochim. Biophys. Acta,162 (1968) 437.
H. A. Lardy, D. Johnson and W. C. McMurray,Arch. Biochem. Biophys.,78 (1958) 587.
R. O. Christiansen, A. Loyter and E. Racker,Biochim. Biophys. Acta,180 (1969) 207.
J. B. Chappell,J. Biol. Chem.,238 (1963) 401.
P. Mitchell, in:Regulation of Metabolic Processes in Mitochondria, J. M. Tager, S. Papa, E. Quagliariello and E. C. Slater (eds.), Elsevier Publishing Co., Amsterdam, 1966, p. 65.
B. C. Pressman and J. K. Park,Biochim. Biophys. Res. Commun.,11 (1963) 182.
P. Mitchell,Chemiosmotic Coupling and Energy Transduction, Glynn Research, Bodmin, Cornwall, 1968.
Author information
Authors and Affiliations
Additional information
Fellow of the Academia Nacional de la Investigación Científica and the Consejo Nacional de Ciencias y Tecnología
Rights and permissions
About this article
Cite this article
Gómez-Puyou, A., Sandoval, F., De Gómez-Puyou, M.T. et al. On the locus of action of Na+ at site I of oxidative phosphorylation. J Bioenerg Biomembr 3, 221–233 (1972). https://doi.org/10.1007/BF01515970
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01515970