Summary
We have combined nuclear magnetic resonance (NMR) imaging on the microscopic scale with chemical shift selection to demonstrate the application of magnetic resonance imaging (MRI) to plant histochemistry. As an example of the method we have obtained separate images of the distribution of reserve oil and anethole in dried fennel mericarps. The technique can be employed to separately image the distribution of aromatics, carbohydrates, oils, water and possibly fatty acids in suitable plant materials.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Abbreviations
- NMR:
-
nuclear magnetic resonance
- MRI:
-
magnetic resonance imaging
- COSY:
-
correlation spectroscopy
- TMS:
-
tetramethylsilane
References
Aguyao JB, Blackband SJ, Schoeniger J, Mattingly MA, Hintermann M (1986) Nuclear magnetic resonance imaging of a single cell. Nature 322: 190–191
Aue WP, Bartholdi E, Ernst RR (1976) Two dimensional spectroscopy: application to nuclear magnetic resonance. J Chem Phys 64: 2229–2246
Bottomley PA, Foster TH, Leue WM (1984) In vivo NMR chemical shift imaging by selective irradiation. Proc Natl Acad Sci USA 81: 6856–6860
Dixon WT (1984) Simple proton spectroscopic imaging. Radiology 153: 189–194
Dumoulin C (1985) A method for chemical shift selective imaging. Magn Reson Med 2: 583–585
Eccles CD, Callaghan PT (1986) High resolution imaging. The NMR microscope. J Magn Reson 68: 393–398
Gassner G, Hohmann B, Deutschmann E (1989) Mikroskopische Untersuchung pflanzlicher Lebensmittel, 5th edn. G Fischer, Stuttgart
Haase A, Frahm J, Hanicke W, Matthaei D (1985)1H NMR chemical shift selective imaging. Phys Med Biol 30: 341–344
Hall LD, Sukumar S, Talaga SL (1984) Chemical shift resolved tomography using frequency selective excitation and suppression of specific resonance. J Magn Reson 56: 275–278
Harrison LG, Luck SD, Munasinghe BDJP, Hall LD (1988) Magnetic resonance imaging approaching microscopic scale: maturation stages ofAcetebularia mediterranea reproductive caps. J Cell Sci 91: 379–388
Hennig J, Friedburg H (1986) Fat and water separation of 0.23 T using simultaneous shift selective imaging. Magn Reson Med 3: 844–848
Jenner CF, Xia Y, Eccles CD, Callaghan PT (1988) Circulation of water within wheat grain revealed by NMR micro-imaging. Nature 336: 399–402
Joseph PM (1985) A spin echo chemical shift MR imaging techniques. J Comput Assist Tomogr 9: 651–658
Kuhn W (1990) NMR microscopy, fundamentals, limits and possible applications. Angew Chem (english edition) 29: 1–19
Melchior H, Kastner H (1974) Gewürze. Paul Parey, Berlin
Morris PC (1986) NMR in medicine and biology. Clarendon Press, Oxford
O'Brien TP, McCully ME (1981) The study of plant structure: principles and selected methods. Termacarphi, Melbourne
Rosen BR, Wedeen VJ, Brady TJ (1984) Selective saturation NMR imaging. J Comput Assist Tomogr 8: 813–818
Sillerud LO, van Hulsteyn DB, Griffey RH (1988) [13C] polarization transfer proton NMR imaging of a sodium [13C] formate phantom at 4.7 Tesla. J Magn Reson 76: 380
Wagner H (1988) Drogen und ihre Inhaltsstoffe, 4th edn. G Fischer, Stuttgart [Stahl E, Deutschmann F, Hohmann B, Reinhard E, Sprecker E, Wagner H, Pharmazeutische Biologie, part 2]
Walter L, Balling A, Zimmermann U, Haase A, Kuhn W (1989) NMR imaging of leaves ofMesembryanthemum crystallinum L. plants grown at high salinity. Planta 178: 524–530
Yannoni CS (1982) High resolution NMR in solids: the CPMAS experiment. Accounts Chem Res 15: 201–208
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sarafis, V., Rumpel, H., Pope, J. et al. Non-invasive histochemistry of plant materials by magnetic resonance microscopy. Protoplasma 159, 70–73 (1990). https://doi.org/10.1007/BF01326636
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01326636