Summary
Some of the relevant biological properties of a rhabdovirus isolated from penaeid shrimps (RPS) were examined. The virus replicated in an established fish cell line, epithelioma papulosum cyprini (EPC) which allowed for the development of a quantitative plaque assay protocol. Virus replication was not inhibited by the DNA antagonist, 5-bromo-2′-deoxyuridine (20 µg/ml). Virus infectivity was sensitive to 20% ethyl ether, low pH, and to 37 °C. The virus showed marked lability to repeated freezing and thawing and storage at −10°C, but was stable at −70°C for several weeks. The virus particle to infectious unit ratio in EPC was found to be 30.
Article PDF
Avoid common mistakes on your manuscript.
References
Lu Y, Nadala ECB Jr, Brock JA, Loh PC (1991) A new virus isolate from infectious hypodermal and hematopoietic necrosis virus (IHHNV)-infected penaeid shrimps. J Virol Methods 31: 189–196
Brown F, Crick J (1979) Natural history of the rhabdoviruses of vertebrates and invertebrate. In: Bishop DHL (ed) Rhabdovirus, vol I. CRC Press, Boca Raton, pp 1–22
Fujiola RS, Loh PC (1978) Recycling of water for irrigation: persistence of enteroviruses in sewage effluent and natural waters receiving the effluent. Water Air Soil Pollut 9: 213–226
Lu Y, Lannan CN, Rohovec JS, Fryer JL (1990) Fish cell lines: establishment and characterization of three new cell lines from grass carp (Ctenopharyngodon idella). In Vitro Cell Dev Biol 26: 275–279
Nadala ECB Jr, Loh PC (1992) Production of high efficiency of plating (EOP) hepatitis A virus in primary African green monkey kidney cells. Proc Soc Ex Biol Med (in press)
Bishop EHL (eds) (1979) Rhabdoviruses, vol I. CRC Press, Boca Raton
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lu, Y., Loh, P.C. Some biological properties of a rhabdovirus isolated from penaeid shrimps. Archives of Virology 127, 339–343 (1992). https://doi.org/10.1007/BF01309596
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309596