Abstract
Two-dimensional electrophoresis of proteins from a recombinant population of anther culture-derived doubled haploid lines identified 4 loci or linkage groups showing a deviation from an expected 1∶1 segregation. It was hypothesized that these markers are linked to genes involved in the process of haploid plant production and that the deviation was due to a selection for alleles conferring higher anther culture response. To check this hypothesis, the anther culturability of 50 of the doubled haploid lines and their two inbred parents was assessed. It was found that 2 of the loci which had a distortion of segregation showed a significant effect on anther culture response, the most efficient allele being the most frequent in both loci. In addition, 2 more markers associated with anther culturability were found. One of the first mentioned 2 loci and one of the latter 2 were found to be linked to genes involved in both embryoid production and subsequent green plant regeneration. The remaining two were linked to genes involved only in green plant regeneration. Of the 4 favorable alleles 3 were inherited from one parent.
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Afele JC, Kannenberg LW (1990) Genetic studies of corn (Zea mays L.) anther culture response. Theor Appl Genet 80:459–464
Agache S, De Buyser J, Henry Y, Snape JW (1988) Studies on the genetic relationship between anther culture and somatic tissue culture abilities in wheat. Plant Breed 100:26–33
Agache S, Bachelier B, De Buyser J, Henry Y, Snape JW (1989) Genetic analysis of anther culture response in wheat using aneuploid, chromosome substitution and translocation lines. Theor Appl Genet 77:7–11
Bentolila S, Hardy T, Guitton G, Freyssinet G (1992) Comparative genetic analyses of F2 plants and anther culture derived plants of maize. Genome 35:575–582
Charmet G, Bernard S (1984) Diallel analysis of androgenetic plant production in hexaploid triticale (xTriticosecale Wittmack). Theor Appl Genet 69:55–61
Cowen NM, Johnson CD, Armstrong K, Miller M, Woosley A, Pescitelli S, Skokut M, Belmar S, Petolino JF (1992) Mapping genes conditioning in vitro androgenesis in maize using RFLP analysis. Theor Appl Genet 84:720–724
Deaton WR, Metz SG, Armstrong TA, Mascia PN (1987) Genetic analysis of the antherculture response of three spring wheat crosses. Theor Appl Genet 74:334–338
Devaux P, Lonnet P, Jean R (1990) Significant higher proportions of 1BL-1RS wheatrye translocation lines among doubled haploid progenies derived from F1 hybrids between translocated and nontranslocated wheat cultivars. In: Nijkamp HJJ, Van der Plas LHW, Van Aartrijk J (eds) Progress in plant cellular and molecular biology. Proc 7th Int Cong Plant Tissue Cell Cult. Kluwer Amsterdam, pp 422–426
Dunwell JM, Francis RJ, Powell W (1987) Anther culture ofHordeurn vulgare L.: a genetic study of microspore callus production and differentiation. Theor Appl Genet 74:60–64
Foroughi-Wehr B, Friedt W (1984) Rapid production of recombinant barley yellow mosaic virus resistantHordeum vulgare lines by anther culture. Theor Appl Genet 67:377–382
Foroughi-Wehr B, Friedt W, Wenzel G (1982) On the genetic improvement of androgenetic haploid formation inHordeum vulgare L. Theor Appl Genet 62:233–239
Graner A, Jahoor A, Schondelmaier J, Siedler H, Pillen K, Fischbeck G, Wenzel G, Herrmann RG (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256
Henry Y, De Buyser J (1990) Wheat anther culture: agronomic performance of doubled haploid lines and the release of a new variety ‘Florin’. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 13. Springer, Berlin Heidelberg New York, pp 285–352
Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34: 437–447
Hou L (1992) Evaluation of barley (Hordeum, vulgare L.) anther culture for breeding applications. PhD thesis, Washington State University, Pullman, Wash., USA
Kleinhofs A, Kilian A, Kudrna D, Bollinger J, Hayes P, Chen FQ, Saghai Maroof MA, Lapitan N, Fenwick A, Blake TK, Kanazin V, Ananiev E, Dahleen L, Liu B, Sorrells M, Heun M, Franckowiak JD, Hoffman D, Skadsen R. Steffenson BJ, Knapp SJ (1993) A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome. Theor Appl Genet 86:705–712
Larsen ET, Tuvesson IKD, Andersen SB (1991) Nuclear genes affecting percentage of green plants in barley (Hordeum vulgare L.) anther culture. Theor Appl Genet 82:417–420
Lazar MD, Baenziger PS, Schaeffer GW (1984) Combining abilities and heritability of callus formation and plantlet regeneration in wheat (Triticum aestivum L.) anther cultures. Theor Appl Genet 68:131–134
Müller G, Borschel H, Vahl U, Wiberg A, Hartel H, Damisch W (1989) Die nutzung der antherenkulturmethode im zuchtprozessvon winterweizen. L Zur androgenesefahigkeit von 1B-1R weizen-roggen-translokationsformen. Plant Breed 102:196–207
Pelletier G, Ilami M (1972) Les facteurs de l'androgenèse in vitro chezNicotiana tabacum. Z Pflanzenphysiol 68:97–114
Petolino JF, Thompson SA (1987) Genetic analysis of anther culture response in maize. Theor Appl Genet 74:284–286
Petolino JF, Jones AM, Thompson SA (1988) Selection for increased anther culture response in maize. Theor Appl Genet 76:157–159
Pickering RA, Devaux P (1992) Haploid production: approaches and use in plant breeding. In: Shewry PR (ed) Barley: genetics, molecular biology and biotechnology. CAB, Wallingford, UK, pp 511–539
Powell W (1988) Diallel analysis of barley anther culture response. Genome 30:152–157
Quimio CA, Zapata FJ (1990) Diallel analysis of callus induction and green plant regeneration in rice anther culture. Crop Sci 30:188–192
SAS (1988) SAS/STAT user's guide, release 6.03 edn. SAS Institute Inc, Cary, N.C., USA
Steel RGD, Torrie JH (1980) Principles and procedures of statistics: a biometrical approach, 2nd edn. McGraw-Hill, New York
Sunderland N, Huang B (1985) Barley anther culture — the switch of programme and albinism. Hereditas [Suppl] 3:27–40
Thompson DM, Chalmers K, Waugh R, Forster BP, Thomas WTB, Caligari PDS, Powell W (1991) The inheritance of genetic markers in microspore-derived plants of barleyHordeum vulgare L. Theor Appl Genet 81:487–492
Wan Y, Rocheford TR, Widholm (1992) RFLP analysis to identifyputative chromosomal regions involved in the anther culture response and callus formation of maize. Theor Appl Genet 85: 360–365
Zivy M, Devaux P, Blaisonneau J, Jean R, Thiellement H (1992) Segregation distortion and linkage studies in microspore-derived double haploid lines ofHordeum vulgare L. Theor Appl Genet 83:919–924
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Communicated by J. W. Snape
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Devaux, P., Zivy, M. Protein markers for anther culturability in barley. Theoret. Appl. Genetics 88, 701–706 (1994). https://doi.org/10.1007/BF01253973
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DOI: https://doi.org/10.1007/BF01253973