Abstract
Values (%) for true digestibility of crude protein and individual amino acids in 20 selected foods were determined by the rat balance (fecal) method. The products were fed as the sole source of protein in diets containing 8% crude protein (N × 6.25). Lowest true protein digestibility values (79–84) were obtained for pinto beans, kidney beans and lentils; intermediate values (89–92) were obtained for chick peas, beef stew, skim milk (over heated), rolled oats, whole wheat cereal, and pea protein concentrate; and highest values (94–100) were obtained for sausage, macaroni-cheese, rice-wheat gluten cereal, skim milk, tuna, soy isolate, peanut butter, chicken frankfurters, beef salami, casein and casein + methionine. In animal foods, peanut butter and soy isolate, the differences between true digestibility of crude protein and most individual amino acids were less than 5%. However, the values for true digestibility of methionine and cystine were up to 44% lower than those of crude protein in pinto beans, kidney beans, lentils, chick peas and pea concentrate. In these legumes, digestibility of crude protein was not a good predictor of digestibility of the limiting amino acids.
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References
Codex Alimentarius Commission Document Alinorm 85/30 (1984) Working Group's Report to the Third Session of CCVP on Methods for Evaluating Protein Quality, Food and Agriculture Organization, Rome, Italy, pp. 32–33
Sarwar G, Peace RW, Botting HR, Brulé D (1989) Relationships between amino acid scores and protein quality indices based on rat growth. Plant Foods Hum Nutr 39: 33–44
Bodwell CE. (Personal communication)
Sarwar G (1984) Available amino acid score for evaluating protein quality of foods. J Assoc Off Anal Chem 67: 623–626
Mongeau R, Sarwar G, Peace RW, Brassard R (1989) Determination of dietary fiber in selected food products and fiber digestibility in rats. Plant Foods Hum Nutr 39: 45–51
Sarwar G, Blair R, Friedman M, Gumbmann R, Hackler LR, Pellett PL, Smith TK (1984) Inter- and intra-laboratory variability in rat growth assays for estimating protein quality of foods. J Assoc Off Anal Chem 67: 976–981
AOAC (1980) Official Methods of Analysis, 13th edn., 7.056 and 14.004. Arlington, VA: Association of Official Analytical Chemists
Sarwar G, Peace RW (1986) Comparisons between true digestibility of total nitrogen and limiting amino acids in vegetable proteins fed to rats. J Nutr 116: 1172–1184
Moore S (1963) On the determination of cystine as cysteic acid. J Biol Chem 238: 235–237
Hugli TE, Moore S (1972) Determination of tryptophan content of alkaline hydrolysates. J Biol Chem 267: 2828–2834
Henry KM (1965) A comparison of biological methods with rats for determining the nutritive value of proteins. Br J Nutr 19: 125–135
Burr HK (1975) Pulse proteins. In: Friedman M (ed) Protein Nutritional Quality of Foods and Feeds—Part 2. New York, NY: Marcel Dekker Inc., pp. 119–134
Sarwar G, Sosulski FW, Bell JM (1977) Availability of amino acids in legumes and legume-wheat blends. Can Inst Food Sci Technol J 10: 31–35
Eggum BO (1968) Nutritional evaluation of proteins by laboratory animals. In: Bender AE, Kihlberg R, Lofquist B, Munck L (eds) Evaluation of Novel Protein Products. New York, NY: Pergamon Press, pp. 117–125
Sarwar G, Bowland JP (1975) Availability of amino acids in wheat cultivars used in diets for weanling rats. Can J Anim Sci 55: 579–586
Taverner MR, Hume ID, Farrell DJ (1981) Availability to pigs of amino acids in cereal grains. 2. Apparent and true ileal digestibility. Br J Nutr 46: 159–171
Marquardt RR, McKirdy JA, Ward T, Campbell LD (1975) Amino acids, hemagglutinin and trypsin inhibitor levels, and proximate analyses of fababeans (vivia faba) and fababean fractions. Can J Anim Sci 55: 421–429
Paquet A, Thresher WC, Swaisgood HE, Catignani GL (1985) Synthesis and digestibility determination of some epimeric tripeptides occurring in dietary proteins. Nutr Res 5: 893–904
Sarwar G, Paquet A, Peace RW (1985) Bioavailability of methionine in some tripeptides occurring in dietary proteins as determined by rat growth. Nutr Res 5: 905–912
Slump P, Van Beek L (1975) Amino acids in feces related to digestibility of food proteins. In: Friedman M (ed) Protein Nutritional Quality of Foods and Feeds, Part 1. New York, NY: Marcel Dekker Inc., pp. 67–78
Bender AE, Mohammadiha H (1981) Low digestibility of legume nitrogen. Proc Nutr Soc 40: 66A
Aw TL, Swanson BG (1985) Influence of tannin onPhaseolus vulgaris protein digestibility and quality. J Food Sci 50: 67–71
Elwell D, Soares JH Jr (1975) Amino acid bioavailability: a comparative evaluation of several techniques. Poult Sci 54: 78–85
Salter DN (1973) The influence of gut microorganisms on utilization of dietery protein. Proc Nutr Soc 32: 65–71
Payne WL, Combs GF, Kifer RR (1968) Investigation of protein quality—ileal recovery of amino acids. Federation Proc 27: 1199–1203
Cho CY, Bayley HS (1972) Amino acid composition of digesta taken from swine receiving diets containing soybean or rapeseed meals as sole source of protein. Can J Physiol Pharmacol 50: 513–522
Taverner MR, Farrell DJ (1981) Availability to pigs of amino acids in cereal grains. 3. A comparison of ileal availability values with faecel, chemical and enzymatic estimates. Br J Nutr 46: 173–180
Sauer WC, Just A, Jorgensen HH, Fekadu M, Eggum BO (1980) The influence of diet composition on the apparent digestibility of crude protein and amino acids at the terminal ileum and overall in pigs. Acta Agric Scan 30: 449–459
Fairweather-Tait SJ, Gee JM, Johnson IT (1982) The influence of cooked kidney beans (Phaseolus vulgaris) on intestinal cell turnover and faecal nitrogen excretion in the rat. Br J Nutr 49: 303–312
Tanksley TD Jr, Knabe DA (1985) Direct measurement of amino acid digestibility in swine. In: Finley WF, Hopkins DT (eds) Digestibility and Amino Acid Availability in Cereals and Oilseeds. St. Paul, Minnesota: American Association of Cereal Chemists, Inc., pp. 259–273
Eggum BO (1985) Digestibility of plant proteins: animal studies. In: Finley WF, Hopkins DT (eds) Digestibility and Amino Acid Availability in Cereals and Oilseeds. St. Paul, Minnesota: American Association of Cereal Chemists Inc., pp. 259–273
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Sarwar, G., Peace, R.W., Botting, H.G. et al. Digestibility of protein and amino acids in selected foods as determined by a rat balance method. Plant Food Hum Nutr 39, 23–32 (1989). https://doi.org/10.1007/BF01092398
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DOI: https://doi.org/10.1007/BF01092398