Abstract
Treatments with copper sulphate (CuSO4), paraquat (PQ) and methidathion (MD) caused tissue damage and stress effects in carp, indicated by the increased lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT), and glutamate dehydrogenase (G1DH) enzyme activities and elevated blood-sugar levels.
Copper sulphate, administered together with PQ and MD, were synergistic in terms of tissue damage and stress effects.
The isoenzyme patterns showed organ-specific tissue damage. The administered chemical and isoenzymes indicating liver damage were detectable in the blood.
The combination of CuSO4 and MD caused focal cell necrosis, which was observable in the liver tissue by light microscopy. Electron microscopic studies revealed the presence of damaged parenchymal cells with electron transparent cytoplasms, myelin figures, and altered mitochondria ER and Golgi.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Annon L (1971) Photometric determination of LDH activity in blood serum. Z Klin Chem Klin Biochem 8:658
Appela E, Markert CL (1961) Dissociation of lactate dehydrogenase into subunits with guanidine hydrochloride. Biochem Biophys Commun 6:1–176
Asztalos B, Nemcsók J (1985) Effect of pesticides on the LDH activity and isoenzyme pattern of carp (Cyprinus carpio L.) sera. Comp Biochem Physiol 82C:217–219
Bell GR (1968) Distribution of transaminases (aminotransferases) in the tissues of pacific salmon (Oncorhynchus), with emphasis on the properties and diagnostic use of glutamic-oxalacetic transaminase. J Fish Res Board Canada 25:1247
Benedeczky I, Biró P, Schaff Zs (1984) The effect of 2,4-D-containing herbicide (Diconirt) on the ultrastructure of carp (Cyprinus carpio) liver cells. Acta Biol Szeged 30:107–125
Benedeczky I, Nemcsók J, Halasy K (1986) Electronmicroscopic analysis of the cytopathological effect of pesticides in the liver, kidney, and gill tissues of carp. Acta Biol Szeged 32:69–91
Chopade HM, Dauterman H (1981) Studies on thein vitro metabolism of methidathion by rat and mouse liver. Pest Biochem and Physiol 15:105
Conning DM, Fletcher K, Swan AAB (1969) Paraquat and related bipyridils. Br Med Bull 25:245
Ferri S, Macha N (1980) Lysosomal enhancement in hepatic cells of a teleost fish induced by cadmium. Cell Biol Internatl Rep 4:367–363
Fisher HK, Humpries M, Bails R (1971) Paraquat poisoning: Recovery from renal and pulmonary damage. Ann Int Med 75:731
Fogh-Andersen N (1982) Lactate dehydrogenase isoenzyme in the diagnosis of myocardial infarction. Clin Biochem 20:291–294
Goldberg E (1966) Lactate dehydrogenase in spermatozoa: Subunits interactionsin vitro. Arch Biochem Biophys 109:134–141
— (1966) Lactate dehydrogenase of trout: Hybridizationin vivo andin vitro. Science 151:1091–1093
Herbert HC, Barth ML, Dodson VN (1970) Isozyme profiles and protein patterns in specific organ damage. Toxicol Appl Pharmacol 16:411–423
Horváth I, Stammer A (1979) Electron microscopical structure of gill lamellas of the Leuciscus Idus with particular regard to the chloride cells and H2S pollution. Acta Biol Szeged 25:133–142
Kristoffersson R, Broberg S, Oikari, Pekkarinen M (1974) Effect of sublethal concentration of phenol on some plasma enzyme activities in the pike (Esox lucius L) in brakish water. Ann Zool Fennici 11:220–223
Markert CL, Faulhaber I (1965) Lactate dehydrogenase isoenzymes pattern of fishes. J Exp Zool 159:319–332
McKim JM, Christensen GM, Hunt EP (1970) Changes in the blood of brook trout (Savenilus fontinalis) after short-term and long-term exposure to copper. J Fish Res Board Canada 27:1883–1889
Nemcsók J, Benedeczky I, Boross L, Asztalos B, Orbán L (1981) Subcellular localisation of transaminase enzymes in fishes and their significance in the detection of water pollution. Acta Biol Szeged 27:9–15
Nemcsók J, Boross L (1982) Comparative studies on the sensitivity of different fish species to metal pollution. Acta Biol Hung 33:23–27
Nemcsók J, Németh Á, Buzás Zs, Boross L (1984) Effect of copper, zinc, and paraquat on acetylcholinesterase activity in carp (Cyprinus carpio L) Aquat Toxicol 5:23–31
Nemcsók J, Orbán L, Asztalos B, Vig É (1987) Accumulation of pesticides in the organs of carp (Cyprinus carpio L) at 4°C and 20°C. Bull Environ Contam Toxicol 39:370–378
Reichenbach-Klinke HH (1972) Histologische und Enzymatische Veränderungen nach Schadstoffeinwirkung beim Fish. Veröf Inst Küst -u Binnenfisch 53:113–120
Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminase. Amer J Clin Path 28:56
Rojik I, Nemcsók J, Boross L (1983) Morphological and biochemical studies on liver, kidney, and gill of fishes. Acta Biol Hung 34:81–92
Rose SM, Smith LL, Wyatt J (1976) The relevance of pentose phosphate stimulation in rat lung to the mechanism of paraquat toxicity. Biochem Pharmacol 25:1763–1767
Schaff Z, Lapis K (1979) Cholestasis. In: Johannessen JV (ed) Electron Microscopy in Human Medicine 8, The Liver. The gallbladder and biliary ducts. McGraw-Hill, New York, pp 89–123
Schmidt E, Schmidt FW (1976) Brief Guide to Practical Enzyme Diagnosis
— (1983) Glutamate dehydrogenase. In: Bergmeyer HU (ed) Methods of Enzymatic Analysis Vol III, Verlab Chemie, Weinheim pp 216–277
Shaklee JB, Kepes KL, Whitt GS (1973) Specialized LDH isoenzymes: The molecular and genetic basis for the unique eye and liver lactate dehydrogenase of teleost fishes. J Exp Zool 185:217–240
Vig É, Orbán L, Nemcsók J, Asztalos B (1987) Einige pathophysiologische Daten beim Karpfen nach der Einvirkung von ausgewählten Fungiziden und Herbiziden. Arch Exper Vet Med 41:491–505
Visi Gy (1984) Study of concentration decrease of pesticides in aquatic model system. Növényvédelem (Hungary) 358–361
Wedemeyer GA (1970) The role of stress in the disease resistance of fishes. In: Shiesko SF (ed) A symposium on disease of fishes and shellfishes. Am Fish Soc Spec 5:30–35
Wrobelski F, La Due JS (1955) Serum glutamate oxaloacetate transaminase as an index of liver cell injury. Proc Soc Exp Biol 90:210
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Asztalos, B., Nemcsók, J.G., Benedeczky, I. et al. The effects of pesticides on some biochemical parameters of carp (Cyprinus carpio L.). Arch. Environ. Contam. Toxicol. 19, 275–282 (1990). https://doi.org/10.1007/BF01056097
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01056097