Summary
Bacillus subtilis DB104, a double mutant which does not synthesize neutral or alkaline proteases, was shown to exhibit some residual proteolytic activity when grown in both batch and continuous cultures. A major protein component responsible for about 70% of extracellular residual protease activity was reversibly deactivated by removal of calcium.
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Longo, A., Snay, J.R., Zimmer, L. et al. Calcium requirements of residual protease in Bacillus subtilis DB104. Biotechnol Lett 10, 649–654 (1988). https://doi.org/10.1007/BF01024719
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DOI: https://doi.org/10.1007/BF01024719