Abstract
A total of 2182 serum samples from 38 patients with surgically confirmed unilocular hydatidosis, 19 clinically assessed patients, 15 patients with parasitic infections other than hydatidosis, 104 hospital outpatients, and 2006 normal Jordanians were serodiagnosed for the presence of IgG antibodies against hydatid fluid, circulating immune complexes (CIC), and/or hydatid circulating antigen (CA). Anti-hydatid IgG antibodies were detected in the sera of 77.4% of patients with hydatid discase and persiste for very long periods postsurgery. As many as 54.1% of patients with hydatidosis had positive levels of CIC, and 16.1% had circulating antigen in their sera. The search for circulating antigen and CIC decreased the number of false-negative hydatid cases from seven to three, and the combined sensitivity of the assays thus increased from 77.4% to 90.3%. Using the immunoblot technique, 16- and <14.4-kDaEchinococcus granulosus-specific bands were detected in sera from 54.1% and 61.5% of patients with hydatid disease who were tested before and after surgery, respectively. The seropositivity rate for anti-hydatid IgG antibodies was 2.4% for the general Jordanian population and 5.8% for hospital outpatients.
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Abdel-Hafez SK, Al-Yaman FM, Said IM (1986) Further studies on the prevalence of hydatidosis in slaughtered animals from North Jordan. Z Parasitenkd 72:89–96
Ajlouni AQ, Saliba EK, Disi AM (1984) Intestinal cestodes of stray dogs in Jordan. Z Parasitenkd 70:203–210
Al-Yaman FM, Assaf LM, Hailat N, Abdel-Hafez SK (1985) Prevalence of hydatidosis in slaughtered animals from North Jordan. Ann Trop Med Parasitol 79:501–506
Al-Yaman FM, Abdel-Hafez SK, Assaf LM, Malkawi FM (1988) Evaluation of various serodiagnostic tests for human hydatidosis. Jpn J Parasitol 37:133–138
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Brooks RG, Sharma BD, Remington JS (1985) Detection ofToxoplasma gondii antigens by a dot immunobinding technique. J Clin Microbiol 21:113–117
Craig PS (1986) Detection of specific circulating antigen, immune complexes and antibodies in human hydatidosis from Turkana (Kenya) and Great Britain, by enzyme-immunoassay. Parasite Immunol 8:171–188
Craig PS, Nelson GS (1984) The detection of circulating antigen in human hydatid disease. Ann Trop Med Parasitol 78:219–227
Craig PS, Zehyle E, Romig T (1986) Hydatid disease: research and control in Turkana. II. The role of immunological techniques for the diagnosis of hydatid disease. Trans R Soc Trop Med Hyg 80:183–192
Dada B, Adegboye DS, Mohammed AN (1981) Experience in Northern Nigeria with counter-current immunoelectrophoresis, double diffusion and indirect haemagglutination tests for diagnosis of hydatid cysts in camels. J Helminthol 55:197–202
Dajani YF (1978) Prevalence of hydatid disease in Syria and Jordan: preliminary results. Trans R Soc Trop Med Hyg 72:320
Dajani YF, Khalaf FH (1981) Hydatidosis and tenuicolosis in sheep and goats of Jordan: comparative study. Ann Trop Med Parasitol 75:175–179
Dajani YF, Shihabi KN (1979) Hydatid disease in Jordan: a review of 50 patients. Dirasat 6:17–27
El-Muhtaseb HH (1984) Surgical management of hydatid cysts of the liver: retrospective study of 75 cases. Jordan Med J 18:35–46
El-Muhtaseb HH, Shihabi KN (1986) Echinococcal cysts in children and youths: retrospective study of 43 cases. Jordan Med J 21:191–204
Farag H, Bout D, Capron A (1975) Specific immunodiagnosis of human hydatidosis by the enzyme-linked immunoabsorbent assay (ELISA). Biomedicine 23:276–278
Gebreel AO, Gilles HM, Percott JE (1983) Studies on the seroepidemiology of endemic disease in Libya. I. Echinococcosis in Libya. Ann Trop Med Parasitol 77:391–397
Goldsmith R, Nahmias J, Schants P, Peleg H, Shtamler B, El-on J (1991) Resurgence of hydatid disease (echinococcosis) in communities in Northern Israel. Trans R Soc Trop Med Hyg 85:98–100
Gottstein B (1984) An immunoassay for the detection of circulating antigens in human echnococcosis. Am J Trop Med Hyg 33:1185–1191
Gottstein B, Eckert J, Woodtli W (1984) Determination of parasite-specific immunoglobulin using the ELISA in patients with echinococcosis treated with mebendazole. Z Parasitenkd 70:385–389
Hira PR, Sheweiki HM, Sibaa R, Behbehani K (1987) Counter-immunoelectrophoresis using an arc 5 antigen for the rapid diagnosis of hydatidosis and comparison with the indirect haemagglutination test. Am J Trop Med Hyg 36:292–297
Hira PR, Behbehani K, Sheweiki H, Abu-Nema T, Soni CR (1988) Hydatid liver disease: problems in diagnosis in the Middle East endemic area. Ann Trop Med Parasitol 82:357–361
Hossain A, Bolbol AS, Chowdhury MN (1985) Serodiagnosis of human hydatid disease in Riyadh, Saudi Arabia. Ann Trop Med Parasitol 79:439–442
Kassimi M, Ali M, Zimmo SK, Khan MA, Anees AM (1983) Pattern of liver disease in the western region of Saudi Arabia. Ann Trop Med Parasitol 77:179–186
Kune GA, Jone T, Sali A (1983) Hydatid disease in Australia. Prevention, clinical presentation and treatment. Med J Aust 15:385–388
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 68:4–8
Macpherson CN, Romig T, Zehle E (1987) Portable ultrasound scanner versus serology in screening of hydatid cysts in nomadic population. Lancet II:259–261
Matossian RM (1977) The immunological diagnosis of human hydatid disease. Trans R Soc Trop Med Hyg 71:101–104
Matossian RM (1979) Some aspects in the immunological diagnosis of human hydatid disease. In: Jidejian YD (ed) Hydatid disease. Dar el-Mashreq, Beirut, pp 27–32
Mlika N, Larouze B, Govedebout C, Braham B, Allegue M, Dazza MC, Dridi M, Gharbi S, Gaumer B, Bchir A, Rousset JJ, Delattre M, Jemalli M (1986) Echotomographic and serologic screening for hydatidosis in a Tunisian village. Am J Trop Med Hyg 35:815–817
Ohlsen S, Zetterstrand K (1985) Detection of circulating immune complexes by PEG precipitation combined with ELISA. J Immunol Methods 77:87–93
Piantelli M, Pozzduli R, Arru E, Musiani P (1977)Echinococcus granulosis: identification of the subunits of the major antigens. J Immunol 119:1382–1386
Richard-Lenolbe D, Smith MD, Lois M, Verroust PJ (1978) Human hydatidosis: evaluation of three serodiagnostic methods, the principal subclass of specific immunoglobulin and detection of circulating immune complexes. Ann Trop Med Parasitol 72:53–56
Rickard MD (1979) The immunological diagnosis of hydatid disease. Aust Vet J 55:99–104
Rickard MD, Lightowlers MW (1986) Immunodiagnosis of hydatid disease. In: Thompson RCA (ed) The biology ofEchinococcus and hydatid disease. George Allen and Unwin, London, pp 217–249
Shekarov AG, Ballad NE, Martynenko VB, Kharitonenko TV (1986) ELISA used to study the epidemiologic process in hydatidosis foci in Yakut, USSR. Med Parazitol Parazit Bolezni 3:6–10
Shennak MM, Tarawneh MS, Amr SS, Alsheik TM, Abu-Rajab MT, Grec SS (1985) Pattern of hepatomegaly in Jordanians: a prospective study of 806 cases. Ann Trop Med Parasitol 79:443–448
Shepherd JC, McManus DP (1987) Specific and cross-reactive antigens ofEchinococcus granulosus hydatid cyst fluid. Mol Biochem Parasitol 25:143–154
Sliman NA (1976) Observations on pulmonary hydatid disease in Jordan. Jordan Med J 11:33–40
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:3350–3354
Yarrow A, Slater PE, Gross EM, Costin C (1991) The epidemiology of echinococcosis in Israel. J Trop Med Hyg 94:261–267
Zodda DM, Abdel-Hafez SK, Philips SM (1983) Characterization of monoclonal antibodies againstSchistosoma mansoni. Am J Trop Med Hyg 32:69–77
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Moosa, R.A., Abdel-Hafez, S.K. Serodiagnosis and seroepidemiology of human unilocular hydatidosis in Jordan. Parasitol Res 80, 664–671 (1994). https://doi.org/10.1007/BF00932950
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DOI: https://doi.org/10.1007/BF00932950