Abstract
In the rabbit, partial urinary bladder outlet obstruction rapidly increases bladder mass and alters several parameters of normal bladder function. Previous experiments on this animal model (Buttyanet al. Neurourol. Urodyn. 11: 225–238, 1992) had identified an early molecular response to partial outlet obstruction that involved the profound induction of mRNA encoding a heat-shock gene, hsp-70, as well as induced expression of mRNA for basic fibroblast growth factor and certain protooncogenes. Numerous physiological studies of the hypertrophied rabbit bladder indicate that the primary stimulus for bladder growth may be the initial overdistension that occurs as the bladder fills following partial outlet obstruction. The present study was undertaken to determine if the sequence and characteristics of gene activation during the recovery following a brief period of overdistension of the rabbit bladder are comparable with the gene activity previously described in association with partial outlet obstruction.
Rabbit bladders were overdistended to 20% above capacity for 1 hr and then relieved. Bladders were recovered from control (untreated) rabbits and from rabbits at 1 hr or at 1, 3 or 5 days following relief of overdistension. RNAs extracted from these tissues were examined by Northern blot assays for a number of different mRNA transcripts previously shown to be altered by partial outlet obstruction. By 1 hr following the relief from acute overdistension, there was already a greater than 10-fold increase in the expression of hsp-70 related transcripts as well as a marked increase in the expression of mRNA encoding bFGF and decreased expression of TGF-β1. Additionally, expression of the early response genes, c-fos and c-jun were induced at this time. By 24 hrs following overdistension, the expression of all genes returned to near control levels. These results were reproduced for individual specimens from two groups of treated rabbits. In summary, a brief interval of overdistension of the rabbit bladder in duces specific alterations in gene activity during the subsequent recovery period that mimics the activity associated with partial outlet obstruction. These results are consistent with the hypothesis that the changes observed following partial outlet obstruction are caused by an initial overdistension of the urinary bladder wall.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Levin RM, High J, Wein AJ: The effect of short-term obstruction on urinary bladder function in the rabbit. J Urol 132: 789–791, 1984
Levin RM, Malkowicz SB, Wein AJ: Basic research models and methods in neuromuscular studies of the lower urinary tract. In: SV Yalla, EJ McGuire, A Elbadawi, JG Blaivas (eds) Neurourology and Urodynamics. Macmillan Publishing Co., New York, 1988, pp 122–146
Longhurst PA, Wein AJ, Levin RM:In-vitro models of bladder contraction and function. Neurourol Urodynam 10: 97, 1991
Malkowicz SB, Wein AJ, Elbadawi A, Van Arsdalen K, Rggieri MR, Levin RM: Acute biochemical and functional alterations in partially obstructed rabbit urinary bladder. J Urol 136: 1324–1329, 1986
Kato K, Wein AJ, Kitada S, Haugaard N, Levin RM: The functional effect of mild outlet obstruction on the rabbit urinary bladder. J Urol 140: 880–884, 1988
Monson FC, McKenna BA, Wein AJ, Levin RM: Effect of outlet obstruction on3H-Thymidine uptake and metabolism: A radiographic and biochemical study. J Urol 148: 158–162, 1992
Buttyan R, Jacobs BZ, Blaivas JG, Levin RM: Early molecular response to rabbit bladder outlet obstruction. Neurourol Urodynam 11: 225–238, 1992
Kato K, Monson FC, Longhurst PA, Wein AJ, Haugaard N, Levin RM: The functional effects of long-term outlet obstruction on the rabbit urinary bladder. J Urol 143: 600–609, 1990
Levin RM, Longhurst PA, Monson FC, Kato K, Wein AJ: Effect of bladder outlet obstruction on the morphology, physiology and pharmacology of the bladder. Prostate supp. 3: 9–26, 1990
Kang J, Wein AJ, Levin RM: Bladder functional recovery following acute overdistension. Neurourol Urodynam 11: 253–261, 1992
Buttyan R, Zakeri Z, Lockshin R, Wolgemuth D: Cascade induction of c-fos, c-myc and heat shock 70K transcripts during regression of the rat ventral prostate gland. Mol Endocrinol 2: 650–657, 1988
Monson FC, Wein AJ, Eika B, Murphy M, Levin RM: Stimulation of the proliferation of rabbit bladder urothekium by partial outlet obstruction and acute overdistension. Neurourol Urodynam (in press)
Levin RM, LOnghurst PA, Monson FC, Haugaard N, Wein AJ: Experimental studies on bladder outlet obstruction. In: H Lepor, RK Lawson (eds) Prostate Diseases. W.B. Saunders Co., Philadelphia, 1993, pp 119–130
Levin RM, Staskin DR, Wein AJ: The effects of acute overdistension of the rabbit urinary bladder. Neurourol Urodynam 2: 63–67, 1983
Tammela T, Wein AJ, Levin RM: Urothelial permeability of the isolated whole bladder. Neurourol Urodynam 12: 39–47, 1993
Karim O, Pienta K, Seki N, Mostwin J: The effect of age on the response of the destrusor to mechanical stimulus: DNA replication and matrix alterations. J Urol 147: 474 (abstract), 1992
Gregory H, Preston BM: The primary structure of human urogastrone. Int J Pep Prot 9: 107–118, 1977
Mount CD, Lukas TJ, Orth DN: Characterization of a high molecular weight form of epidermal growth factor in an extract of human urine. Arch Biochem Biophys 255: 1–7, 1987
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chen, MW., Krasnapolsky, L., Levin, R.M. et al. An early molecular response induced by acute overdistension of the rabbit urinary bladder. Mol Cell Biochem 132, 39–44 (1994). https://doi.org/10.1007/BF00925673
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00925673