Abstract
The anterior segment of the human eye was screened for differences in the lectin-binding patterns of Con A, PNA, GS-I, WGA, SBA, DBA, and UEA-I to enable cell typing for cell-culture purposes. An immunohistochemical technique combining an indirect antibody-lectin method with the avidin-biotin system was used. Con A and WGA were bound by all cells except the conjunctival goblet cells. UEA-I was exclusively bound by both vascular endothelial cells and some corneal and conjunctival epithelial cells. The binding of GS-I, PNA, SBA, and DBA showed an uneven pattern and differed among the cases investigated. The reasons for these differences are not clear. Our results indicate that the usefulness of lectins for cell-typing purposes is restricted and must be determined for every case.
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Ahmed AI, Rahi AHS (1985) Physiological and pathobiological significance of ocular glycoproteins: I. Studies using fluorescein labelled glycine max. Br J Ophthalmol 69:162–170
Barondes SH (1981) Lectins: their multiple endogenous cellular functions. Ann Rev Biochem 50:207–231
Bog-Hansen TC, Freed DLJ (eds) (1988) Lectins — biology biochemistry, clinical biochemistry, vol 6. Proceedings of the 9th Lectin Meeting, Cambridge, 1987. Sigma, St Louis
Cooper NGF, Tarnowski BI, McLaughlin BJ (1987) Lectin affinity of microvillous membranes from the pigmented epithelium of rat retina. Curr Eye Res 6:969–979
Gipson IK, Anderson RA (1980) Effect of lectins on migration of the corneal epithelium. Invest Ophthalmol Vis Sci 19:341–349
Graham RC, Lundholm U, Karnovsky MJ (1965) Cytochemical demonstration of peroxidase activity with 3-amino-9-ethylcarbazole. J Histochem Cytochem 13:150–152
Hicks D, Molday RS (1985) Localization of lectin receptors on bovine photoreceptor cells using dextran-gold markers. Invest Ophthalmol Vis Sci 26:1002–1013
Kawano K, Uehara F, Ohba N (1988) Lectin-cytochemical study on epithelial mucus glycoprotein of conjunctiva and pterygium. Exp Eye Res 47:43–51
Koide H, Suganuma T, Murata F, Ohba N (1986) Ultrastructural localization of lectin receptors in the monkey retinal photoreceptors and pigment epithelium: application of lectin-gold complexes on thin sections. Exp Eye Res 43:343–354
Leathem A, Atkins N (1983) Lectin binding to formalin-fixed paraffin sections. J Clin Pathol 36:747–750
Liener IE, Sharon N, Goldstein IJ (1986) The lectins. Properties, functions, and applications in biology and medicine. Academic Press, Orlando
Lis H, Sharon N (1986) Lectins as molecules and as tools. Ann Rev Biochem 55:35–67
McLaughlin BJ, Boykins LG (1987) Examination of sialic acid binding on dystrophic and normal retinal pigment epithelium. Exp Eye Res 44:439–450
McLaughlin BJ, Wood JG (1980) The localization of lectin binding sites on photoreceptor outer segments and pigment epithelium of dystrophic retinas. Invest Ophthalmol Vis Sci 19:728–742
McLaughlin BJ, Boykins LG, Caldwell PB (1984) Lectin-ferritin binding on dystrophic and normal retinal pigment epithelial membranes. J Neurocytol 13:467–480
McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077–1083
Nemanic MK, Whitehead JS, Elias PM (1983) Alterations in membrane sugars during epidermal differentiation: visualization with lectins and role of glycosidases. J Histochem Cytochem 31:887–897
Ohno J, Fukuyama K, Epstein WL (1989) Dynamic changes of cell-surface glycoconjugates in human palmar epidermis following friction-blisters. Cell Tissue Res 258:403–408
Opas M, Kalnins VI (1985) Distribution of spectrin and lectinbinding materials in surface lamina of RPE cells. Invest Ophthalmol Vis Sci 26:621–627
Panjwani N, Moulton P, Alroy J, Baum J (1986) Localization of lectin binding sites in human, cat, and rabbit corneas. Invest Ophthalmol Vis Sci 27:1280–1284
Prause JU, Jensen OA, Paschides K, Stovhase A, Vangsted P (1989) Conjunctival cell glycoprotein pattern of healthy persons and of patients with primary Sjögren's syndrome — light microscopical investigation using lectin probes. J Autoimmun 2:495–500
Russell P, Sato S (1986) A study of lectin-binding to the waterinsoluble proteins of the lens. Exp Eye Res 42:95–106
Vanden Hoek TL, Goossens W, Knepper PA (1987) Fluorescence-labeled lectins, glycoconjugates, and the development of the mouse AOP. Invest Ophthalmol Vis Sci 28:451–458
Versura P, Maltarello MC, Caramazza R, Laschi R (1989) Mucus alteration and eye dryness. A possible relationship. Acta Ophthalmol 67:455–464
Wells PA, DeSiena-Shaw C, Rice B, Foster CS (1988) Detection of ocular mucus in normal human conjunctiva and conjunctiva from patients with cicatricial pemphigoid using lectin probes and histochemical techniques. Exp Eye Res 46:485–497
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Rittig, M., Brigel, C. & Lütjen-Drecoll, E. Lectin-binding sites in the anterior segment of the human eye. Graefe's Arch Clin Exp Ophthalmol 228, 528–532 (1990). https://doi.org/10.1007/BF00918485
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DOI: https://doi.org/10.1007/BF00918485