Summary
The presence of immunoreactive insulin in saliva and its relationship to plasma immunoreactive insulin was investigated in healthy subjects, newly diagnosed non-obese Type 2 (non-insulin-dependent) diabetic patients and obese non-diabetic subjects, basally and after an oral glucose tolerance test. The mean ± SEM fasting values of plasma and salivary immunoreactive insulin were significantly higher in diabetic patients and obese non-diabetic subjects than in normal volunteers (p<0.05). During the glucose challenge, the increase of salivary insulin was related with that of plasma in the three groups of subjects, with a time lag in normal and obese subjects. In normal volunteers, plasma and salivary peak values were respectively 49.5 ± 13.4 μU/ml (p<0.05 vs obese subjects) at 60 min and 12.0±3.3μU/min (p<0.05 vs obese subjects) at 120 min; in diabetic patients, the values were 51.7 ± 5.6 μU/ml (p<0.05 vs obese subjects) and 14.6±4.1 μU/min at 120 min; in obese subjects, the peak value for plasma insulin was 111.5±40.1 μU/ml at 90 min and for salivary insulin 15.6 ± 5.1 μU/min at 120 min. A positive linear relationship was shown between plasma and salivary insulin during the oral glucose tolerance test. The identity of salivary insulin was assessed by reversed-phase HPLC. We conclude that salivary immunoreactive insulin can be found in Type 2 diabetic patients and in obese non-diabetic subjects, as well as normal volunteers, that plasma and salivary insulin are related after a glucose load, and that differences exist in salivary insulin secretion patterns among the three groups of subjects.
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Sweeney EA, Antoniades HN (1967) The presence of immunologically reactive insulin in parotid saliva and its relation to changes in serum insulin concentration. Vox Sang 13: 54–56
Vallejo G, Mead PM, Gaynor DH, Devlin JT, Robbins DC (1984) Characterization of immunoreactive insulin in human saliva: evidence against production in situ. Diabetologia 27: 437–440
Sweeney EA, Juan CS, Avruskin TW (1979) Turner's syndrome and carbohydrate metabolism. Parotid salivary insulin concentration in normal subjects and in patients with gonadal dysgenesis. Am J Med Sci 277:153–162
Doi K, Kawara A, Fujii S, Mastsuura M, Yoshida Y, Kisara Y, Yoshida M, Kaneko S, Baba S (1978) Insulin like substance in saliva. Igakunoayumi 106: 477–478
National Diabetes Data Group (1979) Classification and diagnosis of diabetes mellitus and other categories of glucose tolerance. Diabetes 28: 1039–1057
Marchetti P, Benzi L, Cecchetti P, Navalesi R (1984) A rapid separation of A14-125I-insulin from heterogeneous iodination mixture by high-performance liquid chromatography (HPLC). J Nucl Med Allied Sci 28:1–4
Navalesi R, Pilo A, Ferrannini E (1978) Kinetic analysis of plasma insulin disappearance in non ketotic diabetic patients and in normal subjects. J Clin Invest 61:197–206
Benzi L, Pezzino V, Marchetti P, Gullo D, Cecchetti P, Masoni A, Vigneri R, Navalesi R (1986) A14-125I-monoiodoinsulin purified by different high-performance liquid chromatography procedures and by polyacrylamide gel electrophoresis: preparation, immunochemical properties and receptor binding affinity. J Chromatogr Biomed Appl 377: 339–344
Rosenzwerg JL, Havronkova J, Lesniak MA, Brownstein M, Roth J (1980) Insulin is ubiquitous in extrapancreatic tissues of rats and humans. Proc Natl Acad Sci USA 77: 572–576
Melani F, Rubenstein AM, Steiner DF (1970) Human serum proinsulin. J Clin Invest 49: 497–507
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Marchetti, P., Benzi, L., Masoni, A. et al. Salivary insulin concentrations in Type 2 (non-insulin-dependent) diabetic patients and obese non-diabetic subjects: Relationship to changes in plasma insulin levels after an oral glucose load. Diabetologia 29, 695–698 (1986). https://doi.org/10.1007/BF00870278
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DOI: https://doi.org/10.1007/BF00870278