Abstract
Citrus pectin (CP) and pH-modified citrus pectin (MCP) are highly branched and non-branched complex polysaccharides, respectively, rich in galactoside residues, capable of combining with the carbohydrate-binding domain of galectin-3. We reported previously that intravenous injection of B16-F1 murine melanoma cells with CP or MCP into syngeneic mice resulted in a significant increase or decrease of lung colonization, respectively (Platt D, Raz A (1992)J Natl Cancer Inst 84:438–42). Here we studied the effects of these polysaccharides on cell-cell and cell-matrix interactions mediated by carbohydrate-recognition. MCP, but not CP, inhibited B16-F1 melanoma cells adhesion to laminin and asialofetuin-induced homotypic aggregation. Both polysaccharides inhibited anchorage-independent growth of B16-F1 cells in semisolid medium, i.e. agarose. These results indicate that carbohydrate-recognition by cell surface galectin-3 may be involved in cell-extracellular matrix interaction and play a role in anchorage-independent growth as well as thein vivo embolization of tumour cells.
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Abbreviations
- CP:
-
natural citrus pectin
- MCP:
-
pH-modified CP
- EHS:
-
Englebreth-Holm Swarm
- CMF-PBS:
-
Ca2+-and Mg2+-free phosphate-buffered saline, pH 7.2
- HRP:
-
horseradish peroxidase
- ABTS:
-
2,2′-azino-di(3-ethylbenzthiazoline sulfonic acid
- DMEM:
-
Dulbecco's modified Eagle's minimal essential medium
- BSA:
-
bovine serum albumin
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Inohara, H., Raz, A. Effects of natural complex carbohydrate (citrus pectin) on murine melanoma cell properties related to galectin-3 functions. Glycoconjugate J 11, 527–532 (1994). https://doi.org/10.1007/BF00731303
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DOI: https://doi.org/10.1007/BF00731303