Abstract
Four polylactides, P-L-LAs 100 KD, 240 KD, 500 KD and a P-DL-LA 400 KD, were testedin vitro by using five cell types. Middle ear, ear canal and nasal septum epithelial cells were used as well as fibroblasts and osteosarcoma cells. The proliferation of cells was studied by culturing on polylactide films and by culturing with media based on artificially aged PLA. The fibroblasts and the osteosarcoma cells were also cultured with media containingL- orD-monomers in different concentrations. Significant differences in cell numbers of polylactide cultures and/or controls were observed. These differences varied per cell type and experimental setting. In general the biocompatibility of the PLAs was satisfactory. Some inhibitory effect on the proliferation of high (10 mg ml−1) monomer concentrations in culture media was seen.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
H. ALEXANDER, A. B. WEISS and J. R. PARSONS,Bull. Hosp. Jt Dis. Orthop. Inst. 46 (1986) 155.
M. CHANAVAZ, F. CHABOT, M. DONAZZAN and M. VERT, in “Biological and biomechanical performance of biomaterials”, edited by P. Christel, A. Meunier and A. J. C. Lee (Elsevier, Amsterdam, 1986) p. 233.
P. ROKKANEN, S. VAINIONPÄÄ, P. TÖRMALA, J. KILPIKARI, O. BÖSTMAN, K. VIHTONEN, J. LAIHO and M. TAMMINMÄKI,Lancet 1 (1985) 1422.
F. R. ROZEMA, R. R. M. BOS, G. BOERING, J. W. LEENSLAG, A. J. PENNINGS and A. B. VERWEIJ, in “Implant materials in biofunction”, “Advances in biomaterials”, Vol. 8, edited by C. de Putter, G. L. de Lange, K. de Groot and A. J. C. Lee (Elsevier, Amsterdam, 1988) p. 251.
C. G. PITT, M. M. GRATZL, G. L. KIMMEL, J. SURTES and A. SCHINDLER,Biomaterials 2 (1981) 215.
D. C. TUNC, in Proceedings of the 9th Annual Meeting of the Society for Biomaterials/15th International Biomaterials Symposium, Birmingham, Alabama, April 1983, edited by R. G. Craig, p. 47.
D. F. WILLIAMS,Engng. Med. 10 (1981) 5.
J. M. SCHAKENRAAD, M. J. HARDONK, J. FEYEN, I. MOLENAAR and P. NIEUWENHUIS,J. Biomed, Mater. Res. 24 (1990) 529.
J. W. LEENSLAG, A. J. PENNINGS, R. R. M. BOS, F. R. ROZEMA and G. BOERING,Biomaterials,8 (1987) 311.
A. S. CHAWLA and T. M. S. CHANG,Biomater. Med. Dev. Artif. Org. 13 (1985/86) 153.
P. S. CHRISTEL, M. VERT, F. CHABOT, Y. ABOLS and J. L. LERAY, in “Biomaterials and biomechanics 1983”, edited by P. Ducheyne, G. Van der Perre and A. E. Aubert (Elsevier, Amsterdam, 1984) p. 1.
D. E. CUTRIGHT and E. E. HUNSUCK,J. Oral. Surg. 33 (1972) 28.
P. CHRISTEL, F. CHABOT and M. VERT, in Proceedings of the 10th Annual Meeting Society Biomaterials* Second World Congress on Biomaterials, Washington, D.C., April 1984, edited by J. M. Anderson, p. 279.
K. JAMSHIDI, S. H. HYON, T. NAKAMURA, Y. IKADA, S. SHIMIZU and T. TERAMATSU, in “Biological and biomechanical performances of biomaterials”, edited by P. Christel, A. Meunier and A. J. C. Lee (Elsevier, Amsterdam, 1986) p. 227.
T. NAKAMURA, S. HITOMI, S. WATANABE, Y. SHIMIZU, K. JAMSHIDI, S. H. HYON and Y. IKADA,J. Biomed. Mater. Res. 23 (1989) 1115.
D. E. CUTRIGHT and E. E. HUNSUCK,J. Oral. Surg. 31 (1971) 134.
D. E. CUTRIGHT, E. E. HUNSUCK and J. D. BEASLY, ibid.29 (1971) 393.
H. J. JOHNSON, S. J. NORTHUP, P. A. SEAGRAVES, M. ATALLAH, P. J. GARVIN, L. LIN and T. D. DARBY,J. Biomed. Mater. Res. 19 (1985) 489.
R. M. RICE, A. F. HEGYELI and S. J. GOURLAY, ibid.12 (1978) 43.
C. A. HOMSY, ibid.4 (1970) 341.
R. E. WILSNACK,Biomater. Med. Dev. Artif. Org. 4 (1976) 235.
W. T. KLÖTZER,Quintessenz 12 (1988) 2147.
S. B. RODAN, Y. IMAI, M. A. THIEDE, G. WESOLOWSKI, D. THOMPSON, Z. BAR-SHAVIT, S. SHULL, K. MANN and G. A. RODAN,Cancer Res. 47 (1987) 4961.
C. A. VAN BLITTERSWIJK, M. PONEC, G. N. P. VAN MUIJEN, M. C. WIJSMAN, H. K. KOERTEN and J. J. GROTE,Acta Otolaryngol. (Stockh.)101 (1986) 453.
D. BAKKER, C. A. VAN BLITTERSWIJK, W. TH. DAEMS and J. J. GROTE,J. Biomed. Mater. Res. 22 (1988) 423.
D. SGOURAS and R. DUNCAN,J. Mater. Sci.: Mater. Med. 1 (1990) 61.
J. B. ULREICH and M. A. CHVAPIL,J. Biomed. Mater. Res. 15 (1981) 913.
F. H. KASTEN, L. F. PINEDA, P. E. SCHNEIDER, H. R. RAWLS and T. A. FOSTER,In Vitro Cell Dev. Biol. 25 (1989) 57.
M. F. SIGOT-LUIZARD, J. L. DUVAL, M. LETORT and M. SIGOT, in “Implant materials in biofunction”, “Advances in biomaterials”, Vol. 8, edited by C. de Putter, G. L. de Lange, K. de Groot and A. J. C. Lee (Elsevier, Amsterdam, 1988) p. 55.
H. J. JOHNSON, S. J. NORTHUP, P. A. SEAGRAVES, P. J. GARVIN and R. F. WALLIN,J. Biomed. Mater. Res. 17 (1983) 571.
D. BAKKER, C. A. VAN BLITTERSWIJK, S. C. HESSELING, J. J. GROTE and W. TH. DAEMS,Biomaterials 9 (1988) 14.
C. WAYMOUTH,In Vitro 6 (1970) 109.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van Sliedregt, A., Radder, A.M., de Groot, K. et al. In vitro biocompatibility testing of polylactides Part I Proliferation of different cell types. J Mater Sci: Mater Med 3, 365–370 (1992). https://doi.org/10.1007/BF00705369
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00705369