Abstract
The degradation of dichloromethane by the pure strainHyphomicrobium GJ21 and by an enrichment culture, isolated from a continuously operating biological trickling filter system, as well as the corresponding growth rates of these organisms were investigated in several batch experiments. By fitting the experimental data to generally accepted theoretical expressions for microbial growth, the maximum growth rates were determined. The effect of NaCl was investigated at salt concentrations varying from 0 to 1000 mM. Furthermore the dichloromethane degradation was investigated separately in experiments in which a high initial biomass concentration was applied. The results show that microbial growth is strongly inhibited by increased NaCl concentrations (50% reduction of μmax at 200–250 mM NaCl), while a certain degree of adaptation has taken place within an operational system eliminating dichloromethane. A critical NaCl concentration for growth of 600 mM was found for the microbial culture isolated from an operational trickling filter, while a value of 375 mM was found for the pure cultureHyphomicrobium GJ21. The substrate degradation appears to be much less susceptible to inhibition by NaCl. Even at 800 mM NaCl relatively high substrate degradation rates are still observed, although this process is again dependent on the NaCl concentration. Here the substrate elimination is due to the maintenance requirements of the microorganisms. The inhibition of the dichloromethane elimination was also investigated in a laboratory scale trickling filter. The results of these experiments confirmed those obtained in the batch experiments. At NaCl concentrations exceeding 600 mM a considerable elimination of dichloromethane was still observed for during several months of operation. These observations indicate that the inhibition of microbial growth offers a significant control parameter against excessive biomass growth in biological trickling filters for waste gas treatment.
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Abbe T (1989) Regulation of solute transport, internal pH and osmolarity inRhodobacter sphearoides. Thesis. Rijksuniversiteit Groningen, Groningen
Beeftink HH, Heijden RTJM & Heijnen JJ (1990) Maintenance requirements: energy supply from simultaneous endogenous respiration and substrate comsumption. FEMS Microbiol. Ecol. 73: 203–210
Bishop PL & Kinner NE (1981) Aerobic fixed-film processes. In: Rehm HJ & Reed G (Eds) Biotechnology, Vols 8, 3. VCH-Verlag, Weinheim
Cooney CL (1981) Growth of microorganisms. In: Rehm HJ & Reed G (Eds) Biotechnology, Vol 1. VCH-Verlag, Weinheim
Diks RMM & Ottengraf SPP (1991a) Verification studies of a simplified model for the removal of dichloromethane from waste gases using a biological trickling filter. Part I. Bioproc. Eng. 6: 93–99
—— (1991b) Verification studies of a simplified model for the removal of dichloromethane from waste gases using a biological trickling filter. Part II. Bioproc. Eng. 6: 131–140
Diks RMM (1992) The removal of dichloromethane from waste gases in a biological trickling filter. Thesis Eindhoven Univ. of Technol., Eindhoven, The Netherlands
Esener AA, Roels JA & Kossen NWF (1981) The bioenergetic correlation of COD to BOD. Biotechnol. Lett. 4: 193–198
Fiechter A (1981) Batch and continuous culture of microbial, plant and animal cells. In: Rehm HJ & Reed G (Eds) Biotechnology, Vols 1, 7. VCH-Verlag, Weinheim
Gälli R & Leisinger T (1985) Specialized bacterial strains for the removal of dichloromethane from industrial waste. Cons. Rec. 8: 91–100
Hartmans S & Tramper J (1991) Dichloromethane removal from waste gases with a trickle-bed bioreactor. Bioproc. Eng. 6: 83–92
Heijnen JJ (1984) Biological industrial waste water treatment minimizing biomass production and maximizing biomass concentration, Thesis Delft Univ. of Technol., Delft, The Netherlands
Herbert D (1985) Some principles of continuous cultures. In: Tunevall G (Ed) Recent Progress in Microbiology, Vol 7. (pp 381–396). Almqvist & Wiksell, Stockholm
Hirsch P (1989) GenusHyphomicrobium. In: Staley JT (Ed) & Murray E (Chairman) Bergey's Manual of Determinative Bacteriology, Vol 3. Williams & Wilkins Comp., Baltimore
Janssen DB, Scheper A & Witholt B (1984) Biodegradation of 2-chloroethanol and 1,2-dichoroethane by pure bacterial cultures. In: Houwink EH & Meer RR van der (Eds) Innovations in Biotechnology, Progr. Ind. Microbiol., Vol 20. Elseviers Science Publishers, Amsterdam
Janssen DB, Kuijk L & Witholt B (1987) Feasibility of specialized microbial cultures for the removal of xenobiotic compounds. Proc. Int. meet. on biol. treatment of ind. waste gases, DECHEMA, 24–26 March, Heidelberg
LaMotta E (1976) Kinetics of growth and substrate uptake in a biological film system. Appl. Environ. Microbiol. 31: 286–293
Levenspiel O (1980) The Monod equation: a revisit and a generalization to product inhibition situations. Biotechnol. Bioeng. 22: 1671–1687
Monod J (1949) The growth of bacterial cultures. Ann. Rev. Microbiol. 3: 371–394
Moore RL (1981) The biology ofHyphomicrobium and other prosthecate, budding bacteria. Ann. Rev. Microbiol. 35: 567–594
Moser A (1981) Kinetics of batch fermentations. In: Rehm HJ & Reed G (Eds) Biotechnology, Vol 2, 14. VCH-Verlag, Weinheim
Niemann D (1993) Biologische Abluftreinigung mit Biofilm-Wirbelschicht Reaktoren. Thesis ETH-Zürich, Zürich, Switserland
Oever AHC van den, Ottengraf SPP & Lith C van (1989) Eindhoven Univ. of Technol., Eindhoven, The Netherlands, Contract research for Clairtech b.v., Utrecht, The Netherlands
Ottengraf SPP, Meesters JJP, Oever AHC van den & Rozema HR (1986) Biological elimination of volatile xenobiotic compounds in biofilters. Bioproc. Eng. 1: 61–69
Pirt SJ (1965) The maintenance energy of bacteria in growing cultures. Proc. R. Soc. London, 163 B: 224–231
Stücki G, Gälli R, Ebershold H & Leisinger T (1981) Dehalogenation of dichloromethane by cell extracts ofHyphomicrobium DM2. Arch. Microbiol. 130: 366–371
Yano T & Koga S (1969) Dynamic behaviour of the chemostat subject to substrate inhibition. Biotechnol. Bioeng. 11: 139–153
Wijngaard AJ van den (1991) Groningen Biotechnol. Center, Univ. of Groningen, The Netherlands, pers. communications
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Diks, R.M.M., Ottengraf, S.P.P. & van den Oever, A.H.C. The influence of NaCl on the degradation rate of dichloromethane byHyphomicrobium sp.. Biodegradation 5, 129–141 (1994). https://doi.org/10.1007/BF00700638
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DOI: https://doi.org/10.1007/BF00700638