Summary
The duration of increase of cerebrospinal vascular permeability in rabbits, in which signs of experimental brain concussion were produced by induction of a brief intracranial pressure pulse, was studied after intravascular injection of a fluorescent permeability indicator at various intervals of up to two days after the sudden loading of the brain.
A marked extravasation of the fluorescent indicator was observed within lateral parts of the brain stem and various parts of the upper cervical cord immediately after the induction of a concussive reaction. Vascular permeability was practicaly normal again as soon as one hour after the induction of the intracranial pressure pulse and 12–18 hours later no fluorescent indicator was observed extravascularly.
The initial increase of vascular permeability in this type of experimental brain concussion is thus of only short duration. This may be a factor of importance with respect to previous observations, that the extravasated indicator does not migrate substantially in the brain stem and cervical cord.
Zusammenfassung
Die Dauer der gesteigerten cerebrospinalen Gefäßpermeabilität bei Kaninchen, an denen Zeichen experimenteller Hirnerschütterung durch kurzen intrakraniellen Druckanstieg erzeugt worden waren, wurde nach intravasaler Injektion eines Fluorescenz-indicators in verschiedenen Zeitabständen bis zu 2 Tage nach der plötzlichen Belastung des Gehirns untersucht.
Eine deutliche Extravasation des fluorescierenden Indicators fand sich in den lateralen Anteilen des Hirnstammes und verschiedenen Abschnitten des rostralen Halsmarks unmittelbar nach Wer Einwirkung einer Concussionsreaktion. Die Gefäßpermeabilität war bereits 1 Std nach Wirkung des intrakraniellen Druckpulses praktisch wieder normal. 12–18 Std später war keine Tracersubstanz extravasal mehr nachweisbar.
Die initiale Steigerung der Gefäßpermeabilität bei dieser Art von experimenteller Gehirnerschütterung ist demnach nur von kurzer Dauer. Das mag von Bedeutung für die früheren Beobachtungen sein, daß der aus den Gefäßen ausgetretene Indicator keine wesentliche örtliche Ausbreitung im Hirnstamm und Halsmark zeigt.
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References
Aird, R. B., L. S. Strait, D. Zealear, andM. Hrenoff: Neurophysiological studies on cerebral concussion. J. Neurosurg.9, 331–347 (1952).
Allen, T. H., andP. D. Orahovats: Spectrophotometric measurement of traces of the dye T-1824 by extraction with cellophane from both blood serum and urine of normal dogs. Amer. J. Physiol.154, 27–38 (1948).
Bakay, L., andI. Haque: Morphological and chemical studies in cerebral edema. I. Cold induced edema. J. Neuropath. exp. Neurol.23, 393–418 (1964).
—, andJ. C. Lee: Cerebral edema. Springfield, Ill.: Ch. Thomas 1965.
Broman, T.: The permeability of the cerebrospinal vessels in normal and pathological conditions. Copenhagen: Munksgaard 1949.
—: On basic aspects of the blood-brain barrier. Acta psychiat. scand.30, 115–124 (1955).
—R. Edström, andO. Steinwall: Technical aspects on dyes and radiotracers in determinations of blood-brain barrier damage. Acta psychiat. scand.36, 69–75 (1961).
Brown, G. W., andM. L. Brown: Cardiovascular responses in experimental cerebral concussion in the rhesus monkey. Arch. Neurol. Psychiat. (Chic.)71, 707–713 (1954).
Cassen, B., andR. Neff: Blood-brain barrier behaviour during temporary concussion. Amer. J. Physiol.198, 1296–1298 (1960).
Chason, J. L., O. U. Fernando, V. R. Hodgson, L. M. Thomas, andE. S. Gurdjian: Experimental brain concussion: morphological findings and a new cytological hypothesis. J. Trauma6, 767–779 (1966).
—,B. F. Haddad, J. E. Webster, andE. S. Gurdjian: Alterations in cell structure following sudden increases in intracranial pressure. J. Neuropath. exp. Neurol.16, 102–107 (1957).
Courville, C. B.: Commotio cerebri, Los Angeles: San Lucas Press 1953.
David, H., K. Franke, andI. Marx: Elektronmikroskopische Befunde an der Großhirnrinde des Hundes nach Sog- und Schlagein wirkungen. Dtsch. Z. ges. gerichtl. Med.56, 177–199 (1965).
—,I. Marx, andH. David: Zur Feinstruktur des experimentell erzeugten subakuten und chronischen Hirnödem. Acta neuropath. (Berl.)9, 212–232 (1967).
Denny-Brown, D.: Cerebral concussion. Physiol. Rev.25, 296–325 (1945).
—, andW. Russell: Experimental cerebral concussion. Brain64, 93–164 (1941).
Dobbing, J.: Blood-brain barrier. Physiol. Rev.41, 130–188 (1961).
Duret, H.: Etudes experimentales et clinique sur les traumatismes cérébraux. Thèse de Paris (1878).
Eichelberger, L., J. J. Kollros, andA. E. Walker: Water, nitrogen and electrolyte content of brain following cerebral concussion. Amer. J Physiol.156, 129–136 (1949).
Faas, F. H., andA. K. Ommaya: Brain tissue electrolytes and water content in experimental concussion in the monkey. J. Neurosurg.28, 137–144 (1968).
Friede, R. L.: Specific cord damage at the atlas level as a pathogenic mechanism in cerebral concussion. J. Neuropath. exp. Neurol.19, 266–279 (1960)
—: Experimental acceleration concussion. Arch. Neurol. (Chic.)4, 449–462 (1961).
Fujita, I.: Potentiality to detect the risk after head injury by measuring the ultrasonic attenuation of the brain. Wakyama med. Rep.11, 75–87 (1966).
Gerlach, I., andH. Becker: Störungen der Bluthirnschranke bei gedeckten stumpfen Schädelhirntraumen. Z. Naturforsch.8b, 578–581 (1953).
Gierke, H. E., von: On the dynamics of some head injury mechanisms. In: Head Injury Conf. Proc. pp. 383–396. Ed.:W. F. Caveness andA. E. Walker. Philadelphia-Toronto: J. B. Lippincott Co. 1966.
Groat, R. A., andI. A. Simmonds: Loss of nerve cells in experimental cerebral concussion. J. Neuropath. exp. Neurol.9, 150–163 (1950).
—,W. F. Windle, andH. W. Magoun: Functional and structural changes in the monkeys brain during and after concussion. J. neurosurg.2, 26–35 (1945).
Gross, A. G.: A new theory on the dynamics of brain concussion and brain injury. J. Neurosurg.15, 548–561 (1958).
Gurdjian, E. S., andH. R. Lissner: Photoelastic confirmation on the presence of shear strains at the craniospinal junction in closed head injury. J. Neurosurg.18, 58–60 (1961).
—— andL. M. Patrick: Concussion-mechanism and pathology. In: Proc. Seventh Stapp Car Crash Conf., pp. 470–482. Ed.:D. M. Severy: Springfield, Ill.: Ch. Thomas 1965.
——J. E. Webster, F. R. Latimer, andB. F. Haddad: Studies on experimental concussion. Relation of physiological effect to time duration of intracranial pressure at impact. Neurology (Minneap.)4, 674–681 (1954).
Güttinger, W.: Der Stoßeffekt auf eine Flüssigkeitskugel als Grundlage einer physikalischen Theorie der Entstehung von Gehirnverletzungen. Z. Naturforsch.5a, 622–628 (1950).
Hamberger, A., andB. Hamberger: Uptake of catecholamines and penetration of trypan blue after blood-brain barrier lesions. A histochemical study. Z. Zellforsch.70, 386–392 (1966).
—, andL. Rinder: Experimental brain concussion: the early effect of sudden increase in intracranial pressure on the succinoxidase activity of isolated neurons and glial cells from the lateral vestibular nucleus of the rabbit. J. Neuropath. exp. Neurol.25, 68–75 (1966).
Hollister, N. R., W. P. Jolley, R. G. Horney, andR. Friede: Biophysics of concussion. WADC Technical Report 58-193. ASTIA Document No. Ad 203385. Aero-Medical Laboratory, U.S. Air Force Wright-Patterson Air Base, Ohio (1958).
Ishii, S., R. Hayner, W. A. Kelly, andJ. P. Evans: Studies of cerebral swelling. II. Experimental cerebral swelling produced by supratentorial extradural compression. J. Neurosurg.16, 152–166 (1959).
Jakob, A.: Experimentelle Untersuchungen über die traumatischen Schädigungen des Zentralnervensystems (mit besonderer Berücksichtigung der Commotio cerebri und Kommotionsneurose). Histologische und Histopathologische Arbeiten (Nissl undAlzheimer).5, 182–358 (1913).
Klatzo, I.: Neuropathological aspects of brain edema. J. Neuropath. exp. Neurol.26, 1–14 (1967).
—J. Miquel, P. Ferris J. Prokop, andD. James: Observations on the passage of the fluorescein labelled serum proteins (FLSP) from the cerebrospinal fluid. J. Neuropath. exp. Neurol.23, 18–35 (1964).
——J. Miquel andR. Otenasek: The application of fluorescenin-labelled serum proteins (FLSP) to the study of vascular permeability in the brain. Acta neuropath. (Berl.)2, 144–160 (1962).
—A. Piraux, andE. J. Laskowski: The relationship between edema, blood-brain barrier and tissue elements in a local brain injury. J. Neuropath. exp. Neurol.17, 548–564 (1958).
—, andO. Steinwall: Observations on cerebrospinal fluid pathways and behaviour of the blood-brain barrier in sharks. Acta Neuropath.5, 161–175 (1965).
—H. Wisniewski, andD. E. Smith: Observations on penetration of serum proteins into the central nervous system. Progr. Brain Res.15, 73–88 (1965).
——,O. Steinwall, andE. Streicher: Dynamics of cold injury edema. In: Proc. Symp. on Brain Edema, Vienna Sept. 11–13, 1965, pp. 554–563. Ed.:I. Klatzo andF. Seitelberger. Wien-New York: Springer 1967.
Klüver, H., andE. Barrera: A method for the combined staining of cells and fibres in the nervous system. J. Neuropath. exp. Neurol.12, 400–403 (1953).
Langfitt, T. W., J. D. Weinstein, andN. F. Kassell: Vascular factors in head injury. Contributions to brain swelling and intracranial hypertension. In: Head Injury Conf. Proc., pp. 172–194. Ed.:W. F. Caveness andA. E. Walker. Philadelphia-Toronto: J. B. Lippincott Co. 1966.
Lee, J. C., andL. Bakay: Ultrastructural changes in the edematous central nervous system. Arch. Neurol. (Chic.)14, 36–47 (1966).
—, andJ. Olszewski: Penetration of radioactive bovine albumin from cerebrospinal fluid into brain tissue. Neurology (Minneap.)10, 814–822 (1960).
Lindgren, S.: Experimental studies of mechanical effects in head injury. Acta chir. scand. Suppl. 360 (1966).
—, andL. Rinder: Experimental studies in head injury. I. Some factors influencing results of model experiments. Biophysik.2, 320–329 (1965).
——: Experimental studies in head injury. II. Pressure propagation in “percussion-concussion”. Biophysik3, 174–180 (1966).
——: Decompression in percussion concussion: effects on “concussive response” in rabbits. J. Trauma7, 493–499 (1967).
Loew, F., andK. Schmalbach: Tierexperimentelle Untersuchungen zur Frage der traumatischen Schädigung der Bluthirnschranke. Dtsch. Z. Nervenheilk.178, 358–364 (1958).
Meessen, H., andJ. Olszewski: A cytoarchitectonic atlas of the rhombencephalon of the rabbit. Basel-New York: S. Karger 1949.
Meyer, J. S., andD. Denny-Brown: Studies of cerebral circulation in brain injury. II. Cerebral concussion. Electroenceph. clin. Neurophysiol.7, 529–544 (1955).
Miller, G.: Cerebral concussion. Arch. Surg.14, 891–916 (1927).
Ommaya, A. K.: Trauma of the nervous system. Ann. Roy. Coll. Surg. Engl.39, 317–347 (1966a).
—: Experimental head injury in the monkey. In: Head Injury Conf. Proc., pp. 260–275. Ed.:W. F. Caveness andA. E. Walker. Philadelphia-Toronto: J. B. Lippincott Co. 1966b.
—D. S. Rockoff, andM. Baldwin: Experimental concussion. J. Neurosurg.21, 249–265 (1964).
Peters, G.: Über gedeckte Gehirnverletzungen (Rindenkontusionen) im Tierversuch. Zbl. Neurochir.8, 172–208 (1943).
Pilcher C.: Experimental cerebral trauma. The fluid content of the brain after trauma to the head. Arch. Surg.35, 512–527 (1937).
—: Experimental cerebral trauma. II. Further observations on the fluid content of the brain following trauma to the head. Surg. Gynec. Obstet.72, 755–757 (1941).
Quadbeck, G., andK. Randerath: Einfluß der Gehirnerschütterung auf den Übertritt von Methylenblau in das Gehirn der Katze. Z. Naturforsch.10b. 168–173 (1955).
Raimondi, A. I., J. P. Evans, andS. Mullan: Studies of cerebral edema. III. Alterations in the white matter. An electronmicroscopic study, using ferritin as labelling compound. Acta neuropath. (Berl.)2, 177–197 (1962).
Rawson, R. A.: The binding of T-1824 and structurally related diazo dyes by the plasma proteins. Amer. J. Physiol.138, 708–717 (1943).
Rengachery, S., D. A. Roth, N. W. Andrew, andV. H. Mark: Alterations of the bloodbrain barrier with hyperventilation. J. Neurosurg.26, 614–618 (1967).
Rinder, L.: Artefactitious extravasation of fluorescent indicators in the investigation of vascular permeability in brain and spinal cord. Acta path. microbiol. scandinav. (In press) (1968).
Rinder, L.: To be published.
Rowbotham, G. F.: Acute injuries of the head. Edinburgh and London: E. S. Livingstone Ltd. 1964.
Scheinker, I. M.: Cerebral swelling. Histopathology, classification and clinical significance of brain edema. J. Neurosurg.4, 255–275 (1947).
Schiller, A. A., R. W. Schayer, andE. L. Hess: Fluoresceinconjugated bovine albumin-physical and biological properties. J. gen. Physiol.36, 489–506 (1953).
Schmidt, H., W. Keck, andO. Grünbeck: Tierexperimentelle Untersuchungen zur formalen Pathogenese der Rindenkontusionsherde. Acta neuropath. (Berl.)4, 46–57 (1964).
Schröder, J. M., andW. Wechsler: Ödem und Nekrose in der grauen und weißen Substanz beim experimentellen Hirntraumen. Acta neuropath. (Berl.)5, 82–111 (1965).
Sellier, K., andF. Unterharnscheidt: Mechanik und Pathomorphologie der Hirnschäden nach stumpfer Gewalteinwirkung auf den Schädel. Hefte Unfallheilk. Heft 76 (1963).
Sjövall, H.: The genesis of skull and brain injuries. Acta path. microbiol. scand. Suppl. 48 (1943).
Steinwall, O., andI. Klatzo: Double tracer methods in studies on blood-brain barrier dysfunction and brain edema. Acta. neurol. scand.41, Suppl. 13, R 31/1–5 (1965).
——: Selective vulnerability of the blood-brain barrier in chemically induced lesions. J. Neuropath. exp. Neurol.25, 542–559 (1966).
Streicher, E., P. J. Ferris, J. D. Prokop, andI. Klatzo: Brain volume and thiocyanate space in local brain injury. Arch. Neurol. (Chic.)11, 444–449 (1964).
Tani, E., andJ. P. Evans: Electron microscope studies of cerebral swelling. I. Studies of the permeability of brain capillaries using ferritin molecules as tracers. Acta neuropath. (Berl.)4, 507–526 (1965).
Thomas, L. M., V. L. Roberts, andE. S. Gurdjian: Impact-induced pressure gradient along three orthogonal axes in the human skull. J. Neurosurg.23, 316–321 (1967).
Unterharnscheidt, F.: Die gedeckten Schäden des Gehirns. Experimentelle Untersuchungen mit einmaliger, wiederholter und gehäufter stumpfer Gewalteinwirkung auf den Schädel. Monographien aus den Gesamtgebieten der Neurologie und Psychiatrie. 103 (1963).
Ward, A. A.: The physiology of concussion. In: Head Injury Conf. Proc., pp. 203–208. Ed.:W. F. Caveness andA. E. Walker. Philadelphia-Toronto. J. B. Lippincott Co. 1966.
White, J. C., J. R. Brooks, J. S. Goldthwait, andD. Adams: Changes in brain volume and blood content after experimental concussion. Ann. Surg.118, 619–633 (1943).
Windle, W. F., R. A. Groat, andC. A. Fox: Experimental structural alterations in the brain during and after concussion. Surg. Gynec. Obstet.79, 561–572 (1944).
—W. A. Rambach, M. De Ramirez R. de Arellano, R. A. Groat, andR. A. Becker: Water content of the brain after concussion and its noncontributory relation to the histopathology of concussion. J. Neurosurg.3, 157–164 (1946).
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The investigation has been supported by grants from the Medical Faculty of the University of Göteborg, Svenska MS-fonden and Trygg-Fylgias Åttioårsfond.
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Rinder, L., Olsson, Y. Studies on vascular permeability changes in experimental brain concussion. Acta Neuropathol 11, 201–209 (1968). https://doi.org/10.1007/BF00692306
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DOI: https://doi.org/10.1007/BF00692306