Summary
Five patients with various brain tumors received bromodeoxyuridine (BrdU), 150–200 mg/m2 i.v.; at the time of craniotomy. Biopsied materials were fixed in 70% ethanol, sectioned, denatured with hydrochloric acid, and reacted with monoclonal antibodies against BrdU. Immunofluorescence and immunocytochemical methods were used to visualize BrdU-labeled nuclei. Our results showed that both methods demonstrated BrdU-labeled nuclei satisfactorily in tissue sections. Thus, BrdU can be used to measure the proliferative potential of human tumors in situ.
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Supported by grant PDT-159 from the American Cancer Society and by grant CA 13525 from the National Cancer Institute
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Nagashima, T., DeArmond, S.J., Murovic, J. et al. Immunocytochemical demonstration of S-phase cells by anti-bromodeoxyuridine monoclonal antibody in human brain tumor tissues. Acta Neuropathol 67, 155–159 (1985). https://doi.org/10.1007/BF00688136
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DOI: https://doi.org/10.1007/BF00688136