Summary
During the first six months of formalin fixation LHA values decrease about 20% in both grey and white matter. RHA does not decrease in the white matter but increases in the grey matter. Determination of LHA and RHA after six months of fixation is accurate enough for quantitative biological and pathological studies.
During development there is an increase and subsequent fall in RHA in grey and in white matter. Maximum values are reached earlier in white than in grey matter.
LHA and RHA were normal in grey and white matter in demyelinating conditions of non-metabolic origin.
Increased LHA values were observed in white matter of metachromatic leucodystrophy, Tay-Sachs disease, and some other leucodystrophies. RHA increase invariably was co-occurring with LHA increase in the white matter but not so in the grey matter.
Zusammenfassung
Während der ersten 6 Monate der Formalinfixierung sinkt der Gehalt an Lipid-Hexosamin (LHA) in der grauen und weißen Hirnsubstanz etwa um 20%. Das Rest-Hexosamin (RHA) sinkt in der weißen Substanz nicht weiter ab, steigt aber in der grauen Substanz. Die Bestimmung von LHA und RHA nach sechsmonatiger Fixation reicht für quantitative biologische und pathologische Untersuchungen aus.
Während der Hirnentwicklung kommt es zu einem Anstieg und späteren Abfall des RHA in der grauen und weißen Substanz. Höchstwerte werden in der weißen Substanz früher als in der grauen erreicht.
LHA und RHA waren normal in grauer und weißer Substanz bei Entmarkungsprozessen nicht-metabolischer Genese.
Erhöhter LHA-Gehalt wurde in der weißen Substanz von metachromatischer Leukodystrophie, Tay-Sachsscher Erkrankung und einigen anderen Leukodystrophien gefunden. RHA-Anstieg ging konstant mit einem LHA-Anstieg in der weißen, nicht aber in der grauen Substanz, either.
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Edgar, G.W.F. Hexosamine in normal and pathological nerve tissue. Acta Neuropathol 13, 182–193 (1969). https://doi.org/10.1007/BF00687030
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DOI: https://doi.org/10.1007/BF00687030