Summary
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1.
Vascular permeability in normal and edematous brain tissue was studied by application of the fluorescein labeled serum proteins (FLSP) as well as by the use of free fluorescein isothiocyanate (FITC) marker.
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2.
The electrophoretic studies demonstrated that the binding capacity of the FITC to albumin was of such degree that morphological observations made after injection of the free tracer can be considered depending primarily on the visualization of the fluorescent serum albumin in the brain tissue.
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3.
The normal brain, with the exception of the several regions described below, showed no evidence of FLSP passage beyond the vascular endothelial lining.
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4.
The choroid plexus, area postrema and the tuber cinereum exhibited green FLSP fluorescence in the connective tissue investitures of the blood vessels but no parenchymatous penetration of the FLSP could be detected in the last two mentioned structures.
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5.
Edema of the white matter, produced by the cortical cold injury in the cat, was associated with the extravascular passage of FLSP which was studied at various stages.
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6.
The appearance of discrete FLSP inclusions in glial cells is interpreted as resulting from pinocytotic uptake of extravasated serum proteins and the significance of pinocytosis in the nervous tissue is discussed.
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7.
The involvement of the microglia in the transport of FLSP from the area of edema towards the pial and ependymal surfaces suggests the importance of this glial element with regard to the transport of soluble substances in the brain tissue.
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8.
It is suggested that the area of edema in the white matter resulting from cold injury may be associated with the presence of an appreciable extracellular compartment.
Zusammenfassung
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1.
Unter Verwendung von fluorescein-markierten Serumproteinen (FLSP) und von freiem Fluorescein-Isothiocyanat (FITC)-Tracer wurde die vasculäre Permeabilität in normalem und ödematösem Hirngewebe untersucht.
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2.
Die elektrophoretischen Untersuchungen ergaben, daß die Bindungskapazität des FITC an Albumin so stark ist, daß man morphologische Beobachtungen nach Injektion mit der freien Tracersubstanz als hauptsächlich von der Sichtbarmachung des fluoreszierenden Serumalbumins im Hirngewebe abhängig betrachten kann.
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3.
Mit Ausnahme der unten angeführten Regionen konnte im normalen gehirn kein Nachweis für die FLSP außerhalb der Gefäßendothelschicht gefunden werden.
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4.
Das Adergeflecht, die Area postrema und das Tuber cinereum zeigten grüne FLSP-Fluorescenz in den bindegewebigen Hüllen der Blutgefäße; jedoch konnte in den beiden letztgenannten Strukturen kein Eindringen der FLSP in das Parenchym festgestellt werden.
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5.
Ein bei der Katze durch eine corticale Kälteschädigung verursachtes Ödem der weißen Substanz ging mit extravasalem Durchtritt von FLSP einher, der in verschiedenen Phasen beobachtet wurde.
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6.
Das Auftreten von geringfügigen FLSP-Einschlüssen in Gliazellen wird auf die pinocytotische Aufnahme von extravasalen Serumproteinen zurückgeführt; die Bedeutung der Pinocytose im Nervengewebe wird erörtert.
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7.
Die Beteiligung der Mikroglia am FLSP-Transport von der Ödemregion zu den pialen und ependymalen Oberflächen läßt auf die wichtige Rolle schließen, die dieses Gliaelement beim Transport löslicher Substanzen im Hirngewebe spielt.
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8.
Die Annahme wird vorgebracht, daß das durch Kälteschädigung entstandene Ödem der weißen Substanz mit dem Vorhandensein eines beträchtlichen extracellulären Raumes in Verbindung steht.
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With 19 Figures in the Text (4 in colour)
From the Section of Neuropathology, Surgical Neurology Branch, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Public Health Service, U.S. Department of Health, Education and Welfare, Bethesda 14, Maryland.
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Klatzo, I., Miquel, J. & Otenasek, R. The application of fluorescein labeled serum proteins (FLSP) to the study of vascular permeability in the brain. Acta Neuropathol 2, 144–160 (1962). https://doi.org/10.1007/BF00685172
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DOI: https://doi.org/10.1007/BF00685172