Summary
Using different hormone-dependent (MCF-7, T-47D) and hormone-independent (MDA-MB-231, Hs-578S, MDA-MB-436) human breast cancer cells, the interconversion estrone (E1) ⇌ estradiol (E2) was explored. The data show very clearly that in the hormone-dependent cells the tendency is to form E2 after incubation with E1, whereas after incubation with E2 most of this estrogen remains unchanged. In the hormone-independent cells, in contrast most of E1 remains E1, while E2 is converted into E1. The tendency of the reductive ⇌ oxidative direction is supported by the analysis of estrogens in the culture medium. To explore the possible action of different drugs on the 17β-hydroxysteroid dehydrogenase (17β-HSD) activity, it was observed that the potent antiestrogen ICI 164,384 inhibits the conversion of E1 to E2, while a lesser effect is observed with Danazol and only weak inhibition is obtained with the progestagen Promegestone (R-5020). It is concluded that the orientation of 17β-HSD activity for the interconversion E1 ⇌ E2 in hormone-dependent and -independent cells is related to the hormonal status of the cells.
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Nguyen, BL., Chetrite, G. & Pasqualini, J.R. Transformation of estrone and estradiol in hormone-dependent and hormone-independent human breast cancer cells. Breast Cancer Res Tr 34, 139–146 (1995). https://doi.org/10.1007/BF00665786
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DOI: https://doi.org/10.1007/BF00665786