Summary
Laser-Doppler flowmetry (LDF) and electromyography (EMG) were used simultaneously for measuring skeletal muscle blood perfusion in relation to static load and fatigue. Percutaneous single-fibre LDF and bipolar surface EMG of the trapezius muscle were performed continuously during a 10-min series of alternating periods of static contractions and rest, each of 1-min duration. The muscle was exposed to static load expressed as shoulder torque, by keeping the arms straight and elevated at 30, 60, 90 and 135°. On-line computer processing of the LDF and EMG signals made possible the interpretation of the relationship between the perfusion and the activity of the muscle. The LDF and root mean square (rms)-EMG were normalized by using the average value of the serial examinations of each individual as a reference value. Spectrum analyses of EMG showed the lowest variability for median frequency (MDF) in the frequency range 10–1000 Hz and mean power frequency (MPF) at 2–1000 Hz. The LDF power spectrum density during low (muscle rest) and high (high-force muscle contraction) perfusion indicated that disturbances were small when measurements were performed during sustained static contraction with as little movement as possible. Vasomotion, i.e. rhythmic variations in the blood flow, were present and showed a frequency of 5–6 cycles · min−1. Application of a tourniquet to the upper arm caused an arrest of the microcirculation in the distally situated brachioradial muscle which was followed by a postischaemic hyperaemia upon removal of the torniquet. In ten healthy men, regression analyses showed positive correlation between rms-EMG and shoulder torque (r=0.77), negative correlation between MPF and arm elevation angle (r= −0.89) indicating accumulated fatigue, and almost positive correlations between LDF and rms-EMG (r=0.65), and between LDF and shoulder angle (r=0.67) when the right trapezius muscle was examined.
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References
Andersen P, Saltin B (1985) Maximal perfusion of skeletal muscle in man. J Physiol (London) 366:233–249
Åstrand PO, Rodahl K (1986) Textbook of work physiology. McGraw Hill, New York
Basmajian JV, DeLuca CV (1985) Muscles alive. Their functions revealed by electromyography. Williams and Wilkins, Baltimore
Cerretelli P, Marconi C, Pendergast D, Meyer M, Heisler N, Piiper J (1984) Blood flow in exercising muscles by Xenon clearance and by microsphere trapping. J Appl Physiol 56:24–30
Chaffin DB, Andersson G (1984) Occupational biomechanics. Wiley, New York, pp 14–52
Hargreaves D, Egginton S, Hudlická O (1990) Changes in capillary perfusion induced by different patterns of activity in rat skeletal muscle. Microvasc Res 40:14–28
Järvholm U, Palmerud G, Karlsson D, Herberts P, Kadefors R (1991) Intramuscular pressure and electromyography in four shoulder muscles. J Orthop Res 9:609–619
Larsson S-E, Öberg PA (1990) Percutaneous recording of muscle blood flow using laser-Doppler single fiber technique. Sixth International Conference in Biomedical Engineering. Goth and Nather, Singapore, pp 549–551
Larsson S-E, Bodegård L, Henriksson KG, Oberg PA (1990) Chronic trapezius myalgia. Morphology and blood flow studied in 17 patients. Acta Orthop Scand 61:394–398
Lassen NA, Lindbjerg J, Munk O (1964) Measurement of blood-flow through skeletal muscle by intramuscular injection of Xenon-133. Lancet 28:686–689
LeVeau B (1977) Biomechanics of human motion. Saunders, Philadelphia
Menger MD, Barker JH, Messmer K (1992) Capillary blood perfusion during postischemic reperfusion in striated muscle. Plast Reconstr Surg 89:1104–1114
Neter J, Wasserman W, Kutner MH (1989) Applied Linear Regression Models, 2nd edn. Irwin, Boston, Mass., p 248ff
Nilsson GE, Tenland T, Öberg PA (1980a) A new instrument for continuous measurement of tissue blood flow by light beating spectroscopy. IEEE Trans Biomed Eng 27:12–19
Nilsson GE, Tenland T, Öberg PA (1980b) Evaluation of a laser-Doppler flowmeter for measurement of tissue blood flow. IEEE Trans Biomed Eng 27:597–604
Öberg PA, Nilsson GE, Tenland T, Holmström A, Lewis DH (1979) Use of a new laser Doppler flowmeter for measurement of capillary blood flow in skeletal muscle after bullet wounding. Acta Chir Scand [Suppl] 489:145–150
Salerud EG, Öberg PA (1987) Single fiber laser-Doppler flowmetry. A method of deep tissue perfusion measurements. Med Biol Eng Comput 25:329–334
Shephard AP, Öberg PÅ (1990) Laser-Doppler blood flowmetry. Kluwer, Boston, Mass
Snedecor GW, Cochran WG (1967) Statistical methods, 6th edn. The Iowa State University Press, Ames, Iowa, p 144ff
Stern MD (1975) In vivo evaluation of microcirculation by coherent light scattering. Nature 254:56–58
Tahmoush AJ, Bowen PD, Bonner RF, Mancini TJ, Engel WK (1983) Laser Doppler blood flow studies during open muscle biopsy in patients with neuromuscular diseases. Neurology 33:547–551
Tyml K, Roman RJ, Lombard JH (1990) Blood flow in skeletal muscle. In: Shephard AP, Öberg PÅ (eds) Laser-Doppler blood flowmetry. Kluwer, Boston, Mass., pp 215–226
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Larsson, SE., Cai, H. & Öberg, P.Å. Continuous percutaneous measurement by laser-Doppler flowmetry of skeletal muscle microcirculation at varying levels of contraction force determined electromyographically. Europ. J. Appl. Physiol. 66, 477–482 (1993). https://doi.org/10.1007/BF00634295
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DOI: https://doi.org/10.1007/BF00634295