Abstract
Calorigenic actions of noradrenaline and glucagon on isolated epididymal fat cells from warmacclimated controls, cold-acclimated and heatacclimated rats were measured by the use of a twin-type conduction microcalorimeter. Both noradrenaline and glucagon stimulated heat production in isolated adipocytes maximally in doses of 1 μg/ml and 10μg/ml, respectively. Maximal responsiveness of adipocytes per unit cell to noradrenaline was not influenced by cold acclimation, while it was reduced by heat acclimation. Maximal response in total epididymal fat cells to noradrenaline was increased in cold acclimation and not changed in heat acclimation at increased numbers of adipocytes in both cold-acclimated and heatacclimated animals. Maximal response per unit cell as well as per total epididymal fat cells to glucagon was increased in cold acclimation and reduced in heat acclimation.
The present results indicate that the modified responses of target adipocytes to noradrenaline and glucagon are involved in the development of temperature acclimation.
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Behrens, W. A., Himms-Hagen, J.: Alterations in skeletal muscle mitochondria of cold-acclimated rats: association with enhanced metabolic response to noradrenaline. J. Bioenerg. Biomembr.9, 41–63 (1977)
Chaffee, R. R. J., Roberts, J. C.: Temperature acclimation in birds and mammals. Ann. Rev. Physiol.33, 155–202 (1971)
Ciaraldi, T. P., Marinetti, G. V.: Hormone action at the membrane level. VIII. Adrenergic receptors in rat heart and adipocytes and their modulation by thyroxine. Biochim. Biophys. Acta541, 334–346 (1978)
Collins, K. J., Weiner, J. S.: Endocrinological aspects of exposure to high environmental temperatures. Physiol. Rev.48, 785–839 (1968)
DiGirolamo, M., Mendlinger, S., Fertig, J. W.: A simple method to determine fat cell size and number in four mammalian species. Am. J. Physiol.221, 850–858 (1971)
Fujita, T., Nonomura, K., Kagami, I., Nishikawa, Y.: Design and testing of a calorimeter for microbiological uses. J. Gen. Appl. Microbiol.22, 43–50 (1976)
Himms-Hagen, J.: Effect of glucagon on the metabolism of adipose tissue. J. Biol. Chem.236, 1023–1027 (1961)
Himms-Hagen, J.: Sympathetic regulation of metabolism. Pharmacol. Rev.19, 367–461 (1967)
Himms-Hagen, J.: Lipid metabolism during cold-exposure and during cold-acclimation. Lipids7, 310–323 (1972)
Himms-Hagen, J., Hagen, P. B.: Action of adrenaline and noradrenaline on metabolic systems. In: Action of hormones on molecular processes (G. Litwack and D. Kritchevsky, eds.), pp. 268–319. New York-London-Sydney: John and Sons, Inc. 1964
Ikemoto, H., Hiroshige, T., Itoh, S.: On possible mechanisms in an increased heat production in adipose tissues of the rat. Jpn. J. Physiol.19, 293–305 (1969)
Joel, C. D.: Stimulation of metabolism of rat brown adipose tissue by addition of lipolytic hormones in vitro. J. Biol. Chem.241, 814–821 (1966)
Kuroshima, A., Doi, K.: Is glucagon involved in cold acclimatization. Experientia32, 473–474 (1976)
Kuroshima, A., Doi, K., Ohno, T.: Role of glucagon in metabolic acclimation to cold and heat. Life Sci.23, 1405–1410 (1978)
Livingston, J. N., Cuatrecasas, P., Lockwood, D. H.: Studies of glucagon resistance in large rat adipocytes:125I-labeled glucagon binding and lipolytic capacity. J. Lipid Res.15, 25–32 (1974)
Madsen, S. N., Sonne, O.: Increase of glucagon receptors in hyperthyroidism. Nature262, 793–795 (1976)
Malbon, C. C., Moreno, F. J., Cabelli, R. J., Fain, J. N.: Fat cell adenylate cyclase and β-adrenergic receptors in altered thyroid states. J. Biol. Chem.253, 671–678 (1978)
Masoro, E. J.: Effect of cold on metabolic use of lipids. Physiol. Rev46, 67–101 (1966)
Petrović, V. M., Marković-Giaja, L.: A comparative study of the calorigenic action of noradrenaline in the rat and ground squirrel adapted to different temperatures. Experientia29, 1295–1296 (1973)
Petrović, V. M., Dabidovic, V., Janć-Sibalić, V.: Catecholamine synthesis, release and excretion and adrenocortical activity in the heat-stressed rat. In: Catecholamines and stress (E. Usdin, R. Kvetnanský, and I. J. Kopin, eds.), pp. 437–443, Oxford-New York-Toronto-Sydney-Paris-Frankfurt: Pergamon Press 1976
Pettersson, B.: CO2-mediated control of fatty acid metabolism in isolated hamster brown-adipose fat cells during norepinephrine stimulation. Eur. J. Biochem.72, 235–240 (1977)
Rodbell, M.: Metabolism of isolated fat cells. I. Effects of hormones on glucose metabolism and lipolysis. J. Biol. Chem.239, 375–380 (1964)
Shum, A., Johnson, G. E., Flattery, K. V.: Influence of ambient temperature on excretion of catecholamines and metabolites. Am J. Physiol.216, 1164–1169 (1969)
Therriault, D. G., Hubbard, R. W., Mellin, D. B.: Endocrine control of fat mobilization in the isolated fat cells of coldexposed rats. Lipids4, 413–420 (1969)
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Kuroshima, A., Kurahashi, M. & Yahata, T. Calorigenic effects of noradrenaline and glucagon on white adipocytes in cold- and heat-acclimated rats. Pflugers Arch. 381, 113–117 (1979). https://doi.org/10.1007/BF00582341
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DOI: https://doi.org/10.1007/BF00582341