Abstract
A series of new neutral hydrogen ion carriers suitable for application in H+-selective microelectrodes is presented. One carrier (ETH 1907) proves to be superior to tridodecylamine currently very much in use. Microelectrodes based on ETH 1907 in an optimized membrane composition exhibit a linear dynamic response function from pH 2 to 9 extended into the acidic range, a response time ≤ 5 s, and a resistance of about 35 GΩ for a tip diameter of about 1 μm. This makes the electrode suitable for measurements at normal physiological intracellular pH as well as in acid physiological media. Measurements using this microelectrode in proximal tubule cells of isolated perfused frog kidney are presented.
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References
Ammann D, Chao P, Simon W (1987) Valinomycin-based K+ selective microelectrodes with low electrical resistance. Neurosci Lett 74:221–226
Ammann D, Lanter F, Steiner RA, Schulthess P, Shijo Y, Simon W (1981) Neutral carrier based hydrogen ion selective microelectrode for extra- and intracellular studies. Anal Chem 53:2267–2269
Bertl A, Felle H, Bentrup F-W (1984) Amine transport in Riccia fluitans. Plant Physiol 76:75–78
Felle H, Bertl A (1986) The fabrication of H+-selective liquid-membrane micro-electrodes for use in plant cells. J Exp Bot 37:1416–1428
Kafoglis K, Hersey SJ, White JF (1984) Microlectrode measurements of K+ and pH in rabbit gastric glands: effect of histamine. Am J Physiol 246:G433-G444
Kraig RP, Pulsinelli WA, Plum F (1985) Heterogeneous distribution of hydrogen and bicarbonate ions during complete brain ischemia. In: Kogure K, Hossmann K-A, Siesjö BK, Welsh FA (eds) Progress in brain research, vol 63. Elsevier, Amsterdam, New York, pp 155–166
Kurkdjian AC, Barbier-Brygoo H (1983) A hydrogen ion-selective liquid-membrane microelectrode for measurement of the vacuolar pH of plant cells in suspension culture. Anal Biochem 132:96–104
Lang F, Messner G, Rehwald W (1986) Electrophysiology of sodium-coupled transport in proximal renal tubules. Am J Physiol 250:F953-F962
Mähr K, Grabner M, Hofer R, Moser H (1983) Histological and physiological development of the stomach inCoregonus sp. Arch Hydrobiol 98:344–353
Messner G, Wang W, Paulmichl M, Oberleithner H, Lang F (1985) Ouabain decreases apparent potassium-conductance in proximal tubules of the amphibian kidney. Pflügers Arch 404:131–137
Moser H (1985) Intracellular pH regulation in the sensory neurone of the stretch receptor of the crayfish (Astacus fluviatilis). J Physiol 362:23–38
Oesch U, Brzózka Z, Xu A, Rusterholz B, Suter G, Pham HV, Welti DH, Ammann D, Pretsch E, Simon W (1986) Design of neutral hydrogen ion carriers for solvent polymeric membrane electrodes of selected pH range. Anal Chem 58:2285–2289
Osuch C, Levine R (1956) The use of organolithium compounds to effect the alkylation of 2- and 4-picoline. J Am Chem Soc 78:1723–1725
Thomas RC (1984) Experimental displacement of intracellular pH and the mechanism of its subsequent recovery. J Physiol 354:3P-22P
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Chao, P., Ammann, D., Oesch, U. et al. Extra- and intracellular hydrogen ion-selective microelectrode based on neutral carriers with extended pH response range in acid media. Pflugers Arch. 411, 216–219 (1988). https://doi.org/10.1007/BF00582318
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DOI: https://doi.org/10.1007/BF00582318