Abstract
The effects of known K+-channel blockers on the electrical properties of bovine lymphatic smooth muscle were investigated using the double sucrose-gap technique. Constant current anodal pulses elicited hyperpolarizing electrotonic potentials (EP's) which were characterised by a “sag” in the potential record. Current/voltage relationship (I/V), which were examined by measuring EP amplitude at the end of 5 s anodal pulses (<30 μA), showed an apparent increase in conductance with increasing hyperpolarization. In the presence of caesium (10 mM), 4-aminopyridine (10 mM) or in the absence of external K+ the sag in the EP was lost and the inward rectification characteristic of the control I/V relationship was abolished. Barium (2.5 mM) also abolished in sag in the EP although TEA (10 mM) had no effect on either EP shape or I/V relationship. Thus it would appear that lymphatic smooth muscle shows inward rectification which is slowly activating and is blocked by some of the known K+-channel blockers or by the removal of external K+.
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Allen, J.M., McHale, N.G. The effect of known K+-channel blockers on the electrical activity of bovine lymphatic smooth muscle. Pflugers Arch. 411, 167–172 (1988). https://doi.org/10.1007/BF00582310
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DOI: https://doi.org/10.1007/BF00582310