Abstract
Bgl-II fragments of the genome of Herpes simplex virus type 2 (HSV-2) HG-52 were cloned into the vector p-Neo and were used to screen the complete HSV-2 genome for regions cross-hybridizing with the genome of HEL cells. Most extensive cross-hybridizing activity was observed with a 530 bpSstII subfragment of the viralBamHI G DNA-fragment (contained inBgl II F), which spans the joint and the viral a-sequence. From a λ-L47 library, a cellular 15 kbHindIII DNA fragment was subcloned in pBR 322 which contained a 1920 bpSstII subfragment having strong cross-hybridizing activity with the 530 bpSst II fragment of HSV-2BamHI G. Within this 1920 bpSst II fragment the cross-hybridizing activity was confined to a 230 bpBgl I/Hpa II subfragment. This 230 bp fragment (including the flanking sequences) was analyzed in comparison to the viral a-sequence. Sequence data revealed a (G+C) content of 66% in the cellular and 81% in the viral DNA fragment, which is mainly determined by an extremely (G+C) rich 16-fold direct repeat (DR2) at the 5′-end. The homology between both DNA-fragments varies between 56% and 79% within the L-S inversion region. Both sequences, furthermore, show homology to the human c-myc protooncogene.
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Kohler, E., Kühn, J., Munk, K. et al. Homology of the HSV-2 “a-sequence” to cellular sequences. Virus Genes 1, 175–189 (1988). https://doi.org/10.1007/BF00555936
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DOI: https://doi.org/10.1007/BF00555936