Abstract
The potential ecotoxicity of fluorescent dyes used in tracing, and their possible effects on human health, were evaluated by reviewing available toxicological information for 12 dyes — fluorescein, Lissamine Flavine FF, Rhodamine WT, Rhodamine B, Sulpho Rhodamine G, Sulpho Rhodamine B, eosin, pyranine, Phorwite BBH Pure, Tinopal 5BM GX, Tinopal CBS-X, and Diphenyl Brilliant Flavine 7GFF — and a dye-intermediate, amino G acid. This evaluation used available toxicological information, test data on analogous substances, and mathematical expressions for biological activity. Based on set criteria for human health and acute ecotoxicity, the evaluation indicated that these tracers have low to moderate levels of concern. The use of these tracers for the study of groundwater flow is appropriate if consideration is given to the overall human health and environmental effects. Their use in the environment requires tracer concentrations not exceeding 1–2 mg 1−1 persisting for a period in excess of 24 h in the groundwater at the point of groundwater withdrawal or discharge. A simple calculated potential dose was used in a comparison of the estimated acute toxicity of Rhodamine WT in rats to the known acute oral toxic dose in humans for several known acutely toxic chemicals. This comparison showed that none of the fluorescent dyes evaluated would present an acutely toxic threat at or substantially above the recommended 2 mg 1−1 concentration.
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References
Abrahart, E. N.: 1968, Dyes and their Intermediates, Pergamon Press, Oxford, 355 pp.
Anliker, R. and Müller, G. eds.: 1975, Fluorescent Whitening Agents. Thieme, Stuttgart. 319 p.
Auer, C. M.: 1989, ‘Structure-activity Evaluation of Florescent Dyes Used in Subsurface Tracing’, Official memorandum from Charles Auer to Ronald Wilhelm, U.S. Environmental Protection Agency.
Auer, C. M. and Gould, D. H.: 1987, ‘Carcinogenicity Assessment and the Role of Structure Activity Relationships (SAR) Analysis Under TSCA Section 5’, Environmental Carcinogenicity Reviews (Journal of Environmental Science Health). C5(1), 29–71.
Auer, C. M., Nabholz and Baetcke, K. P.: 1990, ‘Mode of Action and the Assessment of Chemical Hazards in the Presence of Limited Data: Use of Structure Activity Relationships (SAR) under TSCA Section 5’, Environmental Health perpectives, 87, 183–197.
Behrens, H.: 1986, ‘Water Tracer Chemistry-A Factor Determining Performance and Analytics of Tracers’, International Symposium on Underground Water Tracing Greece, Proceedings Athens, pp. 121–133.
Benoit-Guyod, J. L., Rochat, J., Alary, K., Andre, C. and Taillander, G.: 1979, ‘Corrélations entre propriétes physico chimiques et écotoxicité de traceurs fluorescents xanthéniques’, Toxicological European Research, II, 241–246.
Fetter, C. W.: 1988, Applied Hydrogeology, 2nd ed. Merrill, Columbus, Ohio, 592 pp.
Field, M. S., Quinlan, J. F. and Wilhelm, R. G.: 1994, ‘Selection, Toxicity, and Use of Fluorescent Dyes for Tracing Ground Water: How Much is Enough?’, Ground Water. (in press).
Ford, D. C. and Williams, P. W.: 1989, Karst Geomorphology and Hydrology, Unwin Hymans, Winchester, MA, 601 pp.
Gaspar, E.: 1987, ‘Flowthrough Hydrokarstic Structures’, in: Gaspar, E. (ed.), Modern Trends in Tracer Hydrology vol. II, CRC Press, Boca Raton, FL, 137 pp.
Green, F. J.: 1990, The Sigma-Aldrich Handbook of Stains, Dyes, and Indicators, Sigma-Aldrich Corp., Milwaukee, WI, 776 pp.
Gvirtzman, H. and Gorelich, S. M.: 1991, ‘Dispersion and Advection in Unsaturated Porous Media Enhanced by Anion Exclusion’, Nature, 352, 793–795.
Haskell: 1976, ‘Acute Oral Test of Rhodamine WT, Haskell Laboratory Report NO. 280-76 (unpublished study) 1 p.
Hofstad, L.: 1991, ‘A Method for Tracing On-site Effluent from Failing Septic Systems’, EPA News-Notes: Nonpoint Source no. 12, pp. 17–18.
Little, L. W. and Lamb, J. C.: 1973, ‘Acute Toxicity of 46 Selected Dyes to the Fathead Minnow, Pimephales promelas’, Dyes and the Environment, American Dye Manufacturing Institute, New York, 23 pp. plus appendices.
Marmion, D. M.: 1991, Handbook of U.S. Colorants for Foods, Drugs, and Cosmetics, 3rd ed., Wiley, New York, 576 pp.
Milanović, P. T.: 1981, Karst Hydrogeology, Water Resources Publications, Littleton, Co, 434 pp.
Moltyaner, G., ed.: 1990, Transport and Mass Exchange Processes in Sand and Gravel Aquifers: Field and Modelling Studies. Atomic Energy of Canada Limited, Ottawa, AECL-10308. 2 vol., 866 pp. [Includes 46 papers presented at an international conference and workshop].
Mull, D. S., Liebermann, T. D. Smoot, J. L. and Woosley, Jr. L. H.: 1988, Application of Dye-Tracing Techniques for Determining Solute-Transport Characteristics of Ground Water in Karst Terranes, U.S. Environmental Protection Agency, Atlanta, GA, EPA 904/6-88-001, 103 pp.
Osman, R.: 1990, ‘Structure-Activity Relationships, in: Hood, R. C., ed., Developmental Toxicity: Risk Assessment and the Future, Van Nostrand Reinhold, New York, pp. 195–200.
Quinlan, J. F.: 1986, ‘Recommended Procedure for Evaluating the Effects of Spills of Hazardous Materials on Groundwater Quality in Karst Terranes. Environmental Problems in Karst Terranes and Their Solutions Conference (Bowling Green, KY), Proceedings. National Water Well Association, Dublin, OH, pp. 183–196.
Quinlan, J. F.: 1989, Ground Water Monitoring in Karst Terranes: Recommended protocols & Implicit Assumptions, U.S. Environmental Protection Agency, Environmental Monitoring Laboratory, Las Vegas, nevada, EPA/600/X-89/050. 89 p. [Draft; final version to be released 1995].
Quinlan, J. F., Ewers, R. O. and Field, M. S.: 1988, ‘How to Use Ground Water Tracing to “Prove” that Leakage of Harmful Materials from a Site in a Karst Terrane Will Not Occur’, Environmental Problems in Karst Terranes and Their Solutions Conference (Nashville, Tenn.), Proceedings, National Water Well Association, Dublin, OH, pp. 289–301.
Rand, G. M. and Petrocelli, S. R. eds.: 1985, Glossary in Fundamentals of Aquatic Toxicology: Methods and Applications, McGraw-Hill, Washington, D. C., 666 pp.
Sabatini, D. A. and Austin, T. A.: 1991, ‘Characteristics of Rhodamine WT and Fluorescein as Absorbing Groundwater Tracers, Ground Water 29, 341–349.
Samiullah, Y.: 1990, Prediction of the Environmental Fate of Chemicals, Elsevier, London, 285 pp.
Smart, P. L.: 1984, ‘A Review of the Toxicity of Twelve Fluorescent Dyes Used for Water Tracing’, National Speleological Society Bulletin, 46, 21–33.
Smart, P. L.: 1985, ‘Applications of Fluorescent Dye Tracers in the Planning and Hydrological Appraisal of Sanitary Landfills’, Quarterly Journal of Engineering Geology, 18, 275–286.
Smart, P. L. and Laidlaw, I. M. S.: 1977, ‘An Evaluation in Some Fluorescent Dyes Used For Water Tracing’, Water Resources Research, 13, 15–33.
Smart, P. L., Whitaker, F. and Quinlan, J. F.: 1988, ‘Water Tracing with Fluorescent Dyes, Part I: Field Tests in Granular Aquifer, Soils, and Other Sediments, an Annotated Bibliography’, Turner Designs, Tracer Notes 120, pp. [Available from Turner Designs, 920 W. Maude Ave., Sunnyvale, CA 94086].
Society of Dyers and Colourists and American Association of textile Chemists and Colorists [SDC & AATCC], 1971–88, Colour Index. Blandford, England, 8 vols.
Trudgill, S. T.: 1987, ‘Soil Water Dye Tracing With Special Reference to the Use of Rhodamine WT, Lissamine FF, and amino G acid’, Hydrological Processes, 1, 149–170.
USEPA: 1979, Office of Toxic Substances 8E Status Report. Report Number 8E-0179-0271.
USEPA: 1988, Chemical Profiles for Extremely Hazardous Substances, U.S. Environmental Protection Agency. Washington, D.C., 3 vols.
USEPA: 1989, Exposure Factors Handbook, U.S. Environmental Protection Agency, Office of Health and Environmental Assessment, EPA/600/8-89/043, Washington, D.C.
Whilhelm, R. G.: 1989, Evaluation of dyes used in subsurface tracing. Official memorandum from Ronald Wilhelm to Charles Auer requesting an evaluation of selected fluorescent dyes. U.S. Environmental protection Agency.
Wilson, J. F., Cobb, E. D. and Kilpatrick, F. A.: 1986, Fluorometric Procedures for Dye Tracing. U.S. Geological Survey, Techniques of Water-Resources Investigations, Book 3, Chap. A12, 34 pp. [Revision of 1968 end.].
Zollinger, H.: 1987, Color Chemistry, VCH, New York, N.Y., 367 pp.
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Field, M.S., Wilhelm, R.G., Quinlan, J.F. et al. An assessment of the potential adverse properties of fluorescent tracer dyes used for groundwater tracing. Environ Monit Assess 38, 75–96 (1995). https://doi.org/10.1007/BF00547128
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DOI: https://doi.org/10.1007/BF00547128