Summary
Homogenates of the caudate nucleus of the pig were submitted to differential centrifugation. The 20 000 g and 80 000 g fractions were isolated and a part of them was osmotically shocked. The highest dopamine content per mg protein was found in the intact 80 000 g fraction. Incubation experiments with the intact and the osmotically shocked fractions at 25°C revealed that the particles of the intact 20 000 g fraction took up dopamine; the influx of the amine was not enhanced by addition of ATP and magnesium to the incubation medium. On the other hand after osmotic shock the uptake of dopamine into the particles of this fraction was greatly enhanced by addition of ATP and magnesium. The uptake of dopamine into the particles of both intact and osmotically shocked 80 000 g fractions was likewise enhanced by ATP and magnesium. The uptake in all fractions was not influenced by ouabain. The influx of dopamine into the particles of the intact 80 000 g fraction was competitively inhibited by reserpine (K i 0.96×10−8 M) and prenylamine (K i 1.74×10−8 M). It is concluded that the intact 20,000 g fraction contains intact synaptosomes; the uptake of dopamine is independent of the presence of ATP and magnesium. The shocked 20 000 g fraction and the 80 000 g fractions contain synaptic vesicles; the uptake of dopamine into these vesicles is enhanced by ATP and magnesium.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Carlsson, A., Hillarp, N. A., Waldeck, B.: A Mg++-ATP dependent storage mechanism in the amine granules of the adrenal medulla. Med. exp. (Basel) 6, 47–53 (1962)
Carlsson, A., Waldeck, B.: A fluorimetric method for the determination of dopamine (3-hydroxytyramine). Acta physiol. scand. 44, 293–298 (1958)
De Robertis, E., Pellegrino de Iraldi, A., Rodriquez de Lopez Arnaiz, G., Zieher, L. M.: Synaptic vesicles from the rat hypothalamus. Isolation and norepinephrine content. Life Sci. 4, 193–201 (1965)
Dixon, M., Webb, E. C.: Enzymes. 2nd edn., pp. 327–331. London: Longmans Ltd. 1967
Laverty, R., Michaelson, I. A., Sharman, D. F., Whittaker, V. P.: The subcellular localization of dopamine and acetylcholine in the dog caudate nucleus. Brit. J. Pharmacol. 21, 482–490 (1963)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. I.: Protein measurements with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)
Philippu, A., Beyer, J.: Dopamine and noradrenaline transport into subcellular vesicles of the striatum. Naunyn-Schmiedeberg's Arch. Pharmacol. 278, 387–402 (1973)
Philippu, A., Burkat, U., Becke, H.: Uptake of norepinephrine by the isolated hypothalamic vesicles. Life Sci. 7, 1009–1017 (1968)
Philippu, A., Heyd, W.: Release of dopamine from subcellular particles of the striatum. Life Sci. 9, 361–373 (1970)
Schwartz, A., Bachelard, H. S., McIlwain, H.: The sodium stimulated adenosine-triphosphatase activity and other properties of cerebral microsomal fractions and subfractions. Biochem. J. 84, 626–637 (1962)
Author information
Authors and Affiliations
Additional information
This work was supported by the Deutsche Forschungsgemeinschaft and the Stiftung Volkswagenwerk.
Rights and permissions
About this article
Cite this article
Philippu, A., Matthaei, H. & Lentzen, H. Uptake of dopamine into fractions of pig caudate nucleus homogenates. Naunyn-Schmiedeberg's Arch. Pharmacol. 287, 181–190 (1975). https://doi.org/10.1007/BF00510449
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00510449