Summary
The availability of sequential DNA phosphates to bind toluidine blue molecules after acid hydrolysis was studied in normally shaped and misshaped spermatozoa from subfertile and highly fertile bulls. The aim was to associate induced spermatozoal metachromasia with infertility. Some few normally and abnormally shaped cells from highly fertile bulls exhibited an induced metachromasia after being treated with 4N HCl for 10–30 min at 25°C prior to staining. Subfertile bulls contained 12 times as many metachromatic spermatozoa as highly fertile animals. The induced toluidine blue metachromasia is suggested as a rapid and simple method for detecting bull spermatozoa bearing an anomalous DNA-protein complex. This nucleoprotein complex was found to be more frequent in subfertile bulls.
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References
Andersson KA, Kjellstrand PTT (1975) A study of DNA depolymerisation during Feulgen acid hydrolysis. Histochemistry 43:123–130
Evenson DP, Darzynkiewicz Z, Melamed MR (1980) Relation of mammalian sperm chromatin heterogeneity to fertility. Science 210:1131–1133
Gledhill BL (1966) Studies on the DNA content dry mass and optical area of morphologically normal and abnormal bull spermatozoal heads. Acta Vet Scand 7:1–20
Gledhill BL (1970) Changes in nuclear stainability associated with spermateliosis, spermatozoal maturation, and male fertility. In: Wied GL (ed) Introduction to quantitative cytochemistry II. Academic Press, New York, pp 125–151
Gledhill BL, Gledhill MP, Rigler Jr R, Ringertz NR (1966) Changes in deoxyribonucleoprotein during spermiogenesis in the bull. Exp Cell Res 41:652–665
Kjellstrand PTT (1977) Temperature and acid concentration int he search for optimum Feulgen hydrolysis conditions. J Histochem Cytochem 25:129–134
Lison L (1955) Variation de la basophilie pendant la maturation de spermatozoide chez le rat et sa signification histochimique. Acta Histochem 2:47–67
Marushige Y, Marushige K (1974) Properties of chromatin isolated from bull spermatozoa. Biochim Biophys Acta 340:498–508
Mello MLS, Vidal BC (1973) Linear dichroism and anomalous dispersion of birefringence on sperm heads. Acta Histochem 45:109–114
Mello MLS, Vidal BC (1977) Changes in anisotropic properties and nuclear stainability during spermatogenesis in the grasshopper, Staurorhectus longicornis Giglio-Toss. Adv Invert Reprod 1:75–83
Miura A, Ohba Y (1967) Structure of nucleohistone. III. Interaction with toluidine blue. Biochim Biophys Acta 145:436–445
Sandritter W, Jobst K, Rakow L, Bosselmann K (1965) Zur Kinetik der Feulgenreaktion bei verlängerter Hydrolysezeit. Cytophotometrische Messungen im sichtbaren und ultravioletten Licht. Histochemie 4:420–437
Toepfer K (1970) Die Thiazinfarbstoffe. Prog Histochem Cytochem 1:277–354
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Mello, M.L.S. Induced metachromasia in bull spermatozoa. Histochemistry 74, 387–392 (1982). https://doi.org/10.1007/BF00493438
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DOI: https://doi.org/10.1007/BF00493438